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  4. ACTR1B Recombinant Monoclonal Antibody

ACTR1B Recombinant Monoclonal Antibody

  • 中文名稱：
    ACTR1B Recombinant Monoclonal Antibody
  • 貨號：
    CSB-RA115875A0HU
  • 規格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in: HEK293 whole cell lysate(20μg),MCF-7 whole cell lysate(20μg), PC-3 whole cell lysate(20μg), U-87MG whole cell lysate(20μg),U-251MG whole cell lysate(20μg),Jurkat whole cell lysate(20μg),Hela whole cell lysate(20μg), Mouse brain tissue lysate(20μg)
      All lanes: ACTR1B antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/40000 dilution
      Predicted band size: 42 kDa
      Observed band size: 42 kDa
      Exposure time：2min
    • IHC image of CSB-RA115875A0HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • IHC image of CSB-RA115875A0HU diluted at 1:100 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • Immunofluorescence staining of Hela cell with CSB-RA115875A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Overlay Peak curve showing Hela cells stained with CSB-RA115875A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

產品詳情

  • Uniprot No.：
  • 基因名：
    ACTR1B
  • 別名：
    Actin related protein 1B antibody; Actin-related protein 1B antibody; ACTR 1B antibody; ACTR1B antibody; ACTY_HUMAN antibody; ARP 1B antibody; ARP1 actin related protein 1 homolog B antibody; ARP1 actin related protein 1 homolog B centractin beta (yeast) antibody; ARP1 actin related protein 1 homolog B centractin beta antibody; ARP1 actin related protein 1 yeast homolog B centractin beta antibody; ARP1 yeast homolog B antibody; ARP1B antibody; Beta centractin antibody; Beta-centractin antibody; Centractin beta (Yeast) antibody; Centractin beta antibody; CTRN 2 antibody; CTRN2 antibody; PC 3 antibody; PC3 antibody
  • 反應種屬：
    Human, Mouse
  • 免疫原：
    A synthesized peptide derived from human ACTR1B
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    14D5
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB, IHC, IF, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:2000
    IHC 1:50-1:200
    IF 1:50-1:200
    FC 1:50-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    Component of a multi-subunit complex involved in microtubule based vesicle motility. It is associated with the centrosome.
  • 基因功能參考文獻：
    1. Data show that 4-oxo-4-HPR inhibited tubulin polymerization and modulated gene expression of spindle aberration associated genes Kif 1C, Kif 2A, Eg5, Tara, tankyrase-1, centractin, and TOGp. PMID: 19996280
    2. Down-regulation of beta-centractin is associated with hepatocellular carcinoma immune escape PMID: 17619203
  • 亞細胞定位：
    Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
  • 蛋白家族：
    Actin family, ARP1 subfamily
  • 數據庫鏈接：

    HGNC: 168

    OMIM: 605144

    KEGG: hsa:10120

    STRING: 9606.ENSP00000289228

    UniGene: Hs.98791



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