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β-hydroxybutyryl-HIST1H1D (K75) Antibody

  • 中文名稱:
    β-hydroxybutyryl-HIST1H1D (K75)兔多克隆抗體
  • 貨號:
    CSB-PA010379OA75bhbHU
  • 規(guī)格:
    ¥660
  • 圖片:
    • Western Blot
      Positive WB detected in: 293 whole cell lysate, A549 whole cell lysate, K562 whole cell lysate (all treated with 30mM sodium butyrate for 4h)
      All lanes: HIST1H1D antibody at 1.3µg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 23 kDa
      Observed band size: 23 kDa
    • Immunocytochemistry analysis of CSB-PA010379OA75bhbHU diluted at 1:10 and staining in Hela cells (treated with 50mM sodium 3-hydroxybutyrate for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品名稱:
    Rabbit anti-Homo sapiens (Human) HIST1H1D Polyclonal antibody
  • Uniprot No.:
  • 基因名:
    HIST1H1D
  • 別名:
    H1 histone family member 3 antibody; H1.3 antibody; H13_HUMAN antibody; H1F3 antibody; HIST1 H1D antibody; HIST1H1D antibody; Histone 1 H1d antibody; Histone cluster 1 H1d antibody; Histone H1.3 antibody; Histone H1c antibody; MGC138176 antibody
  • 宿主:
    Rabbit
  • 反應(yīng)種屬:
    Human
  • 免疫原:
    Peptide sequence around site of β-hydroxybutyryl-Lys (75) derived from Human Histone H1.3
  • 免疫原種屬:
    Homo sapiens (Human)
  • 標(biāo)記方式:
    Non-conjugated
  • 克隆類型:
    Polyclonal
  • 抗體亞型:
    IgG
  • 純化方式:
    Antigen Affinity Purified
  • 濃度:
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液:
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 產(chǎn)品提供形式:
    Liquid
  • 應(yīng)用范圍:
    ELISA, WB, ICC
  • 推薦稀釋比:
    Application Recommended Dilution
    WB 1:100-1:1000
    ICC 1:10-1:100
  • Protocols:
  • 儲存條件:
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期:
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途:
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能:
    Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation.
  • 基因功能參考文獻(xiàn):
    1. Results show that histone H1.3, was identified only in non-neoplastic MCF-10A breast cells but not in metastatic MDA-MB-231 breast cancer cells . PMID: 26209608
    2. Histone H1 organizes and maintains an extensive protein-protein interaction network in the nucleolus required for nucleolar structure and integrity. PMID: 25584861
    3. overexpression of histone cluster 1 is associated with recurrence in meningiomas. PMID: 20685720
  • 亞細(xì)胞定位:
    Nucleus. Chromosome. Note=According to PubMed:15911621 more commonly found in euchromatin. According to PubMed:10997781 is associated with inactive chromatin.
  • 蛋白家族:
    Histone H1/H5 family
  • 數(shù)據(jù)庫鏈接:

    HGNC: 4717

    OMIM: 142210

    KEGG: hsa:3007

    STRING: 9606.ENSP00000244534

    UniGene: Hs.136857



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