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lexA Antibody

Rare Species
  • 中文名稱：
    lexA兔多克隆抗體
  • 貨號：
    CSB-PA363985HA01ENV
  • 規格：
    ￥440
  • 圖片：
    • Western Blot
      Positive WB detected in Recombinant protein
      All lanes: lexA antibody at 3.4μg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      predicted band size: 39 kDa
      observed band size: 39 kDa
  • 其他：

產品詳情

  • 產品描述：

    The LexA antibody is prepared through the immunization of rabbits with the peptide corresponding to amino acids 1-202 of the recombinant E.coli LexA repressor protein and further purification from rabbit antiserum by protein G. It is a polyclonal antibody and exists as an unconjugated IgG. Its purity reaches up to 95%. It only reacts with the LexA protein of E.coli-origin. And this anti-LexA antibody has been validated for use in ELISA and WB analyses. In E.coli, the transcriptional repressor LexA controls the SOS response, which is a coordinated cellular response to DNA damage. The LexA protein inhibits gene expression by binding as a dimer to a 16-mer consensus sequence CTG-N10-CAG named SOS boxes.

  • 產品名稱：
    Rabbit anti-Escherichia coli lexA Polyclonal antibody
  • Uniprot No.：
  • 基因名：
    lexA
  • 別名：
    lexA antibody; exrA antibody; spr antibody; tsl antibody; umuA antibody; b4043 antibody; JW4003LexA repressor antibody; EC 3.4.21.88 antibody
  • 宿主：
    Rabbit
  • 反應種屬：
    Escherichia coli
  • 免疫原：
    Recombinant Escherichia coli LexA repressor protein (1-202AA)
  • 免疫原種屬：
    Escherichia coli
  • 標記方式：
    Non-conjugated

    本頁面中的產品，lexA Antibody (CSB-PA363985HA01ENV)，的標記方式是Non-conjugated。對于lexA Antibody，我們還提供其他標記。見下表：

    可提供標記
    標記方式 貨號 產品名稱 應用
    HRP CSB-PA363985HB01ENV lexA Antibody, HRP conjugated ELISA
    FITC CSB-PA363985HC01ENV lexA Antibody, FITC conjugated
    Biotin CSB-PA363985HD01ENV lexA Antibody, Biotin conjugated ELISA
  • 克隆類型：
    Polyclonal
  • 抗體亞型：
    IgG
  • 純化方式：
    >95%, Protein G purified
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:5000
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. Binds to the 16 bp palindromic sequence 5'-CTGTATATATATACAG-3'. In the presence of single-stranded DNA, RecA interacts with LexA causing an autocatalytic cleavage which disrupts the DNA-binding part of LexA, leading to derepression of the SOS regulon and eventually DNA repair. Implicated in hydroxy radical-mediated cell death induced by hydroxyurea treatment.The SOS response controls an apoptotic-like death (ALD) induced (in the absence of the mazE-mazF toxin-antitoxin module) in response to DNA damaging agents that is mediated by RecA and LexA.
  • 基因功能參考文獻：
    1. The conformational flexibility of unbound LexA is the key element in establishing a co-ordinated SOS response. PMID: 21576225
    2. LexA regulated genes exhibit phenotypic heterogeneity as high level expression is observed in only a small subpopulation. PMID: 21070632
    3. NMR analysis of LexA catalytic domain in its active conformation PMID: 15929009
    4. oligomerization of LexA is proposed to be a possible regulation mechanism of the Escherichia coli SOS regulon in response to environmental conditions resulting in acidic intracellular pH. Structural changes on LexA repressor promoted by acidic pH. PMID: 16701697
    5. LexA binds to and represses the promoter of antC of coliphage N15. PMID: 17586637

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  • 蛋白家族：
    Peptidase S24 family
  • 數據庫鏈接：


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