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  4. lepA Antibody, HRP conjugated

lepA Antibody, HRP conjugated

Rare Species
  • 中文名稱：
    lepA兔多克隆抗體, HRP偶聯
  • 貨號：
    CSB-PA350834LB01ENV
  • 規格：
    ￥880
  • 其他：

產品詳情

  • 產品名稱：
    Rabbit anti-Escherichia coli lepA Polyclonal antibody
  • Uniprot No.：
  • 基因名：
    lepA
  • 別名：
    lepA antibody; b2569 antibody; JW2553Elongation factor 4 antibody; EF-4 antibody; EC 3.6.5.n1 antibody; Ribosomal back-translocase LepA antibody
  • 宿主：
    Rabbit
  • 反應種屬：
    Escherichia coli
  • 免疫原：
    Recombinant Escherichia coli Elongation factor 4 protein (1-599AA)
  • 免疫原種屬：
    Escherichia coli
  • 標記方式：
    HRP
  • 克隆類型：
    Polyclonal
  • 抗體亞型：
    IgG
  • 純化方式：
    >95%, Protein G purified
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back-translocation proceeds from a post-translocation (POST) complex to a pre-translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP-dependent manner.
  • 基因功能參考文獻：
    1. The last 44 C-terminal amino acids of EF4 form a sub-domain within the C-terminal domain that is important for GTP-dependent function on the ribosome. PMID: 25712150
    2. Thus, EF4 has two opposing functions-at low-to-moderate levels of stress, the protein is protective by allowing stress-paused translation to resume; at high-levels of stress, EF4 helps bacteria self-destruct. PMID: 25491353
    3. (review) EF4 triggers back-translocation of the elongating ribosome, causing the translation machinery to move one codon backwards along the mRNA. PMID: 23662805
    4. Elevated intracellular Mg(2+) concentrations or low temperature inhibit protein synthesis, probably because of formation of aberrant elongating ribosomal states. We suggest that EF4 binds to these stuck ribosomes and remobilizes them PMID: 21300907
    5. EF4-dependent back-translocation proceeds from a post-translocation (POST) complex to a pre-translocation (PRE) complex via a four-step kinetic scheme (i.e., POST-->I(1)-->I(2)-->I(3)-->PRE PMID: 20045415
    6. LepA protein, present in all bacteria and mitochondria, is a third elongation factor required for accurate and efficient protein synthesis. PMID: 17110332
    7. The crystal structure of LepA from Escherichia coli at 2.8-A resolution, was determined. PMID: 18362332

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  • 亞細胞定位：
    Cell inner membrane; Peripheral membrane protein; Cytoplasmic side.
  • 蛋白家族：
    TRAFAC class translation factor GTPase superfamily, Classic translation factor GTPase family, LepA subfamily
  • 數據庫鏈接：


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