在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

gag-pol Antibody

Rare Species
  • 中文名稱:
    gag-pol兔多克隆抗體
  • 貨號:
    CSB-PA361030XA01MHK
  • 規格:
    ¥440
  • 圖片:
    • Western Blot
      Positive WB detected in Recombinant protein
      All lanes: gag-pol antibody at 1:2000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 23.2 kDa
      Observed band size: 26 kDa
  • 其他:

產品詳情

  • Uniprot No.:
  • 基因名:
    gag-pol
  • 別名:
    Gag-Pol polyprotein (Pr180gag-pol) [Cleaved into: Matrix protein p15 (MA); RNA-binding phosphoprotein p12 (pp12); Capsid protein p30 (CA); Nucleocapsid protein p10 (NC-pol); Protease p14 (PR) (EC 3.4.23.-); Reverse transcriptase/ribonuclease H p80 (RT) (EC 2.7.7.49) (EC 2.7.7.7) (EC 3.1.26.4); Integrase p46 (IN) (EC 2.7.7.-) (EC 3.1.-.-)] gag-pol
  • 反應種屬:
    Moloney murine leukemia virus (isolate Shinnick) (MoMLV)
  • 免疫原:
    Recombinant Moloney murine leukemia virus (isolate Shinnick) (MoMLV) POL protein (560-631aa)
  • 免疫原種屬:
    Moloney murine leukemia virus (isolate Shinnick) (MoMLV)
  • 標記方式:
    Non-conjugated
  • 克隆類型:
    Polyclonal
  • 抗體亞型:
    IgG
  • 純化方式:
    Protein G
  • 濃度:
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液:
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 產品提供形式:
    Liquid
  • 應用范圍:
    ELISA, WB
  • Protocols:
  • 儲存條件:
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期:
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途:
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能:
    Plays a role in budding and is processed by the viral protease during virion maturation outside the cell. During budding, it recruits, in a PPXY-dependent or independent manner, Nedd4-like ubiquitin ligases that conjugate ubiquitin molecules to Gag-Pol, or to Gag-Pol binding host factors. Interaction with HECT ubiquitin ligases probably links the viral protein to the host ESCRT pathway and facilitates release.; Targets Gag and gag-pol polyproteins to the plasma membrane via a multipartite membrane binding signal, that includes its myristoylated N-terminus. Also mediates nuclear localization of the pre-integration complex.; Constituent of the pre-integration complex (PIC) which tethers the latter to mitotic chromosomes. This allows the integration of the viral genome into the host DNA.; Forms the spherical core of the virion that encapsulates the genomic RNA-nucleocapsid complex.; Involved in the packaging and encapsidation of two copies of the genome. Binds with high affinity to conserved UCUG elements within the packaging signal, located near the 5'-end of the genome. This binding is dependent on genome dimerization. Acts as a nucleic acid chaperone which is involved in rearrangement of nucleic acid secondary structures during gRNA retrotranscription.; The aspartyl protease mediates proteolytic cleavages of Gag and Gag-Pol polyproteins during or shortly after the release of the virion from the plasma membrane. Cleavages take place as an ordered, step-wise cascade to yield mature proteins. This process is called maturation. Displays maximal activity during the budding process just prior to particle release from the cell (Potential). Cleaves the translation initiation factor eIF4G leading to the inhibition of host cap-dependent translation.; RT is a multifunctional enzyme that converts the viral dimeric RNA genome into dsDNA in the cytoplasm, shortly after virus entry into the cell. This enzyme displays a DNA polymerase activity that can copy either DNA or RNA templates, and a ribonuclease H (RNase H) activity that cleaves the RNA strand of RNA-DNA heteroduplexes in a partially processive 3' to 5' endonucleasic mode. Conversion of viral genomic RNA into dsDNA requires many steps. A tRNA binds to the primer-binding site (PBS) situated at the 5' end of the viral RNA. RT uses the 3' end of the tRNA primer to perform a short round of RNA-dependent minus-strand DNA synthesis. The reading proceeds through the U5 region and ends after the repeated (R) region which is present at both ends of viral RNA. The portion of the RNA-DNA heteroduplex is digested by the RNase H, resulting in a ssDNA product attached to the tRNA primer. This ssDNA/tRNA hybridizes with the identical R region situated at the 3' end of viral RNA. This template exchange, known as minus-strand DNA strong stop transfer, can be either intra- or intermolecular. RT uses the 3' end of this newly synthesized short ssDNA to perform the RNA-dependent minus-strand DNA synthesis of the whole template. RNase H digests the RNA template except for a polypurine tract (PPT) situated at the 5' end of the genome. It is not clear if both polymerase and RNase H activities are simultaneous. RNase H probably can proceed both in a polymerase-dependent (RNA cut into small fragments by the same RT performing DNA synthesis) and a polymerase-independent mode (cleavage of remaining RNA fragments by free RTs). Secondly, RT performs DNA-directed plus-strand DNA synthesis using the PPT that has not been removed by RNase H as primers. PPT and tRNA primers are then removed by RNase H. The 3' and 5' ssDNA PBS regions hybridize to form a circular dsDNA intermediate. Strand displacement synthesis by RT to the PBS and PPT ends produces a blunt ended, linear dsDNA copy of the viral genome that includes long terminal repeats (LTRs) at both ends.; Catalyzes viral DNA integration into the host chromosome, by performing a series of DNA cutting and joining reactions. This enzyme activity takes place after virion entry into a cell and reverse transcription of the RNA genome in dsDNA. The first step in the integration process is 3' processing. This step requires a complex comprising the viral genome, matrix protein and integrase. This complex is called the pre-integration complex (PIC). The integrase protein removes 2 nucleotides from each 3' end of the viral DNA, leaving recessed CA OH's at the 3' ends. In the second step that requires cell division, the PIC enters cell nucleus. In the third step, termed strand transfer, the integrase protein joins the previously processed 3' ends to the 5' ends of strands of target cellular DNA at the site of integration. The last step is viral DNA integration into host chromosome.
  • 基因功能參考文獻:
    1. Findings indicate that reverse transcriptase is a relatively passive enzyme, able to polymerize on structured templates by exploiting their thermal breathing. PMID: 29165701
    2. X-ray crystal structure of the N-terminal region of Moloney murine leukemia virus integrase and its implications for viral DNA recognition has been reported. PMID: 28066922
    3. transmembrane protein associates with the assembling core particle through the R-peptide before budding and that this is the mechanism by which the budding virus acquires the envelope proteins. [TM R-peptide] PMID: 16690922
    4. analysis of how the murine leukaemia virus Env opens for membrane fusion PMID: 18800055
  • 亞細胞定位:
    [Gag-Pol polyprotein]: Virion. Host cell membrane; Lipid-anchor. Host late endosome membrane; Lipid-anchor. Host endosome, host multivesicular body.; [Matrix protein p15]: Virion.; [Capsid protein p30]: Virion.; [Nucleocapsid protein p10-Pol]: Virion.; [Protease]: Virion.; [RNA-binding phosphoprotein p12]: Host cytoplasm.
  • 數據庫鏈接:

    KEGG: vg:2193424



主站蜘蛛池模板: 亚洲国产精品久久久久婷婷软件| 嫩草av久久伊人妇女超级a| av无码一区二区三区| 国产熟妇与子伦hd| 亚洲日韩片无码中文字幕| 在线|国产精品女主播阳台| 亚洲中文字幕无码av在线| 久久精品国产99国产精品澳门| 青草视频在线观看视频| 久久久久久国产精品mv| 国产性色av一区二区三区| 五月丁香六月综合av| 国产精品爽爽久久久久久竹菊| 18禁男女污污污午夜网站免费暖暖| 亚洲性无码av在线dvd| 麻麻张开腿让我爽了一夜| 最新69国产成人精品视频免费| 亚洲乱码在线卡一卡二卡新区豆瓣| 九色porny丨自拍视频| 极品少妇被啪到呻吟喷水| 精品久久久久久无码不卡| 无码人妻丝袜视频在线播免费| 无码国产色欲xxxxx视频| 欧美最猛性xxxxx免费| 中文精品一卡2卡3卡4卡国色| 欧美亚洲熟妇一区二区三区| 精品亚洲麻豆1区2区3区| 欧美巨波霸乳影院| 4480yy私人精品国产| 亚洲人成图片小说网站| 蜜芽久久人人超碰爱香蕉| 欧美va免费高清在线观看| 久久综合av色老头免费观看| 中文字幕日韩精品有码视频 | 国产精品亚洲二区在线播放| 亚洲色精品88色婷婷七月丁香| 欧美老熟妇欲乱高清视频 | 亚洲香蕉网久久综合影视| 亚洲第一狼人伊人av| 精品无码一区二区三区在线| 永久免费看啪啪的网站|