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fbpA Antibody

Rare Species
  • 中文名稱：
    fbpA兔多克隆抗體
  • 貨號：
    CSB-PA18279A0Rb
  • 規格：
    ￥440
  • 圖片：
    • Western Blot
      Positive WB detected in Recombinant Mycobacterium tuberculosis Antigen 85-A protein
      All lanes: Antigen 85-A antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 34 kDa
      Observed band size: 34 kDa
  • 其他：

產品詳情

  • 產品描述：

    The polyclonal antibody against fbpA was developed by CUSABIO through immunizing rabbits with a synthetic peptide that corresponds to amino acids 53-331 of the recombinant Ag85A protein, a Diacylglycerol acyltransferase/mycolyltransferase in Mycobacterium tuberculosis. After immunization, the antiserum from the rabbits was subjected to protein G affinity chromatography to purify the anti-fbpA antibody, which exhibits a purity level of 95%. The antibody is present as an unconjugated IgG.

    This fbpA polyclonal antibody only reacts with Mycobacterium tuberculosis fbpA, which mediates triacylglycerol (TAG) formation with long-chain acyl-CoA as the acyl donor and 1,2-dipalmitoyl-sn-glycerol (1,2-dipalmitin) as the acyl acceptor and has a preference for C26:0-CoA over C18:1-CoA. This fbpA antibody is suitable for the detection of fbpA protein in ELISA and WB applications.

  • 產品名稱：
    Rabbit anti-Mycobacterium tuberculosis fbpA Polyclonal antibody
  • Uniprot No.：
  • 基因名：
    fbpA
  • 別名：
    fbpA antibody; mpt44 antibody; Rv3804c antibody; MTV026.09cDiacylglycerol acyltransferase/mycolyltransferase Ag85A antibody; DGAT antibody; EC 2.3.1.122 antibody; EC 2.3.1.20 antibody; Acyl-CoA:diacylglycerol acyltransferase antibody; Antigen 85 complex A antibody; 85A antibody; Ag85A antibody; Fibronectin-binding protein A antibody; Fbps A antibody
  • 宿主：
    Rabbit
  • 反應種屬：
    Mycobacterium tuberculosis
  • 免疫原：
    Recombinant Mycobacterium tuberculosis Diacylglycerol acyltransferase/mycolyltransferase Ag85A protein (53-331AA)
  • 免疫原種屬：
    Mycobacterium tuberculosis
  • 標記方式：
    Non-conjugated

    本頁面中的產品，fbpA Antibody (CSB-PA18279A0Rb)，的標記方式是Non-conjugated。對于fbpA Antibody，我們還提供其他標記。見下表：

    可提供標記
    標記方式 貨號 產品名稱 應用
    HRP CSB-PA18279B0Rb fbpA Antibody, HRP conjugated ELISA
    FITC CSB-PA18279C0Rb fbpA Antibody, FITC conjugated
    Biotin CSB-PA18279D0Rb fbpA Antibody, Biotin conjugated ELISA
  • 克隆類型：
    Polyclonal
  • 抗體亞型：
    IgG
  • 純化方式：
    >95%, Protein G purified
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:1000-1:5000
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    The antigen 85 proteins (FbpA, FbpB, FbpC) are responsible for the high affinity of mycobacteria for fibronectin, a large adhesive glycoprotein, which facilitates the attachment of M.tuberculosis to murine alveolar macrophages (AMs). They also help to maintain the integrity of the cell wall by catalyzing the transfer of mycolic acids to cell wall arabinogalactan, and through the synthesis of alpha,alpha-trehalose dimycolate (TDM, cord factor). They catalyze the transfer of a mycoloyl residue from one molecule of alpha,alpha-trehalose monomycolate (TMM) to another TMM, leading to the formation of TDM. FbpA mediates triacylglycerol (TAG) formation with long-chain acyl-CoA as the acyl donor and 1,2-dipalmitoyl-sn-glycerol (1,2-dipalmitin) as the acyl acceptor. It has a preference for C26:0-CoA over C18:1-CoA.
  • 基因功能參考文獻：
    1. Ag85A protein can induce strong specific humoral and Th1 type cellular immune responses. PMID: 29727142
    2. A screen for protein-protein interactions reveals a functional link between the virulence-associated lipid transporter LprG and the mycolyltransferase antigen 85A. PMID: 28276676
    3. Using a prime and pull approach, lentivector vaccines expressing Ag85A induce immunogenicity but fail to induce protection against Mycobacterium bovis bacillus Calmette-Guerin challenge in mice. PMID: 26095282
    4. The authors show that the enzyme possesses acyl-CoA:diacylglycerol acyltransferase (DGAT) activity in addition to its well-known function as mycolyltransferase. PMID: 21819455
    5. findings revealed 6 proteins with high-affinity for the fbpA promoter, and 7 with specificity the fbpC promoter PMID: 19959397
    6. analysis of enzymatic hydrolysis of trehalose dimycolate releases free mycolic acids during mycobacterial growth in biofilms PMID: 20375425
    7. In addition to immune recognition of Ag85A whole protein, peptide-mapping studies identified four immunogenic Ag85A peptides, which induced Th-1 immune responses in individual sarcoidosis subjects, suggesting a role in sarcoidosis immunopathogenesis. PMID: 17357846

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  • 亞細胞定位：
    Secreted, cell wall. Cytoplasm.
  • 蛋白家族：
    Mycobacterial A85 antigen family
  • 數據庫鏈接：

    KEGG: mtu:Rv3804c

    STRING: 83332.Rv3804c



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