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ZDHHC1 Antibody

  • 中文名稱:
    ZDHHC1兔多克隆抗體
  • 貨號:
    CSB-PA837848LA01HU
  • 規格:
    ¥440
  • 圖片:
    • Western Blot
      Positive WB detected in: A549 whole cell lysate(20μg), Hela whole cell lysate(20μg), U251 whole cell lysate(20μg), 293 whole cell lysate(20μg), Mouse Brain whole cell lysate(20μg),Mouse Spleen tissue lysate(20μg)
      All lanes: ZDHHC1 antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 48, 55 kDa
      Observed band size: 55 kDa
      Exposure time: 10s
    • IHC image of CSB-PA837848LA01HU diluted at 1:100 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody
    • Immunofluorescence staining of U251 cell with CSB-PA837848LA01HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Immunofluorescence staining of U251 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Immunofluorescence staining of A549 cell with CSB-PA837848LA01HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Immunofluorescence staining of A549 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他:

產品詳情

  • 產品名稱:
    Rabbit anti-Homo sapiens (Human) ZDHHC1 Polyclonal antibody
  • Uniprot No.:
  • 基因名:
    ZDHHC1
  • 別名:
    ZDHHC1; C16orf1; ZNF377; Palmitoyltransferase ZDHHC1; DHHC domain-containing cysteine-rich protein 1; Zinc finger DHHC domain-containing protein 1; DHHC-1; Zinc finger protein 377
  • 宿主:
    Rabbit
  • 反應種屬:
    Human, Mouse
  • 免疫原:
    Synthesized peptide derived from human ZDHC1 AA range: 81-131
  • 免疫原種屬:
    Homo sapiens (Human)
  • 克隆類型:
    Polyclonal
  • 抗體亞型:
    IgG
  • 純化方式:
    Antigen affinity purification
  • 濃度:
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液:
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  • 產品提供形式:
    Liquid
  • 應用范圍:
    ELISA, WB, IHC, IF
  • 推薦稀釋比:
    Application Recommended Dilution
    WB 1:1000-1:3000
    IHC 1:50-1:200
    IF 1:20-1:100
  • Protocols:
  • 儲存條件:
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期:
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途:
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能:
    Palmitoyltransferase that could catalyze the addition of palmitate onto various protein substrates. Has a palmitoyltransferase activity toward NCDN and regulates NCDN association with endosome membranes through this palmitoylation.; Has also a palmitoyltransferase activity-independent function in DNA virus-triggered and CGAS-mediated innate immune response. Functions as an activator of STING1 by promoting its cGAMP-induced oligomerization and the recruitment of downstream signaling components.
  • 基因功能參考文獻:
    1. In an expression screen for proteins that can activate the IFNB1 promoter, authors identified the ER-associated protein ZDHHC1 as a positive regulator of virus-triggered, MITA/STING-dependent immune signaling. PMID: 25299331
  • 亞細胞定位:
    Endosome membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus.
  • 蛋白家族:
    DHHC palmitoyltransferase family
  • 組織特異性:
    Widely expressed with significant expression in heart, brain, placenta, lung, liver, kidney, testis, thymus and small intestine. Expressed at lower levels in adult pancreas and lung.
  • 數據庫鏈接:

    HGNC: 17916

    KEGG: hsa:29800

    STRING: 9606.ENSP00000340299

    UniGene: Hs.624541



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