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  4. YWHAH Antibody

YWHAH Antibody

  • 中文名稱：
    YWHAH兔多克隆抗體
  • 貨號(hào)：
    CSB-PA02174A0Rb
  • 規(guī)格：
    ￥440
  • 圖片：
    • Western blot
      All lanes: YWHAH antibody at 2µg/ml + Mouse spleen tissue
      Secondary
      Goat polyclonal to rabbit IgG at 1/10000 dilution
      Predicted band size: 29 kDa
      Observed band size: 29 kDa
    • Western Blot
      Positive WB detected in: HepG2 whole cell lysate, Mouse kidney tissue, Mouse brain tissue, Mouse thymus tissue
      All lanes: YWHAH antibody at 4.5µg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 29 kDa
      Observed band size: 29 kDa
    • Immunohistochemistry of paraffin-embedded human prostate tissue using CSB-PA02174A0Rb at dilution of 1:20
    • IHC image of CSB-PA02174A0Rb diluted at 1:400 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • IHC image of CSB-PA02174A0Rb diluted at 1:400 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of Hela cells with CSB-PA02174A0Rb at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品名稱：
    Rabbit anti-Homo sapiens (Human) YWHAH Polyclonal antibody
  • Uniprot No.：
  • 基因名：
  • 別名：
    14 3 3 protein eta antibody; 14-3-3 eta antibody; 14-3-3 protein eta antibody; 1433F_HUMAN antibody; Brain protein 14-3-3; eta isoform antibody; HGNC:12853 antibody; Protein AS1 antibody; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein 1 antibody; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein eta polypeptide antibody; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein; eta isoform antibody; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein; eta antibody; Tyrosine 3/tryptophan 5 monooxygenase activation protein eta polypeptide antibody; YWHA 1 antibody; YWHA1 antibody; Ywhah antibody
  • 宿主：
    Rabbit
  • 反應(yīng)種屬：
    Human, Mouse
  • 免疫原：
    Recombinant Human 14-3-3 protein eta protein (4-246AA)
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated

    本頁(yè)面中的產(chǎn)品，YWHAH Antibody (CSB-PA02174A0Rb)，的標(biāo)記方式是Non-conjugated。對(duì)于YWHAH Antibody，我們還提供其他標(biāo)記。見(jiàn)下表：

    可提供標(biāo)記
    標(biāo)記方式 貨號(hào) 產(chǎn)品名稱 應(yīng)用
    HRP CSB-PA02174B0Rb YWHAH Antibody, HRP conjugated ELISA
    FITC CSB-PA02174C0Rb YWHAH Antibody, FITC conjugated
    Biotin CSB-PA02174D0Rb YWHAH Antibody, Biotin conjugated ELISA
  • 克隆類型：
    Polyclonal
  • 抗體亞型：
    IgG
  • 純化方式：
    >95%, Protein G purified
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, WB, IHC, IF
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:2000
    IHC 1:20-1:500
    IF 1:50-1:200
  • Protocols：
  • 儲(chǔ)存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1.
  • 基因功能參考文獻(xiàn)：
    1. Results show that YWHAZ amplification indicates a better survival trend in bladder cancer. Its knockdown promotes both in vitro and in vivo tumorigenesis in bladder cancer and may be a novel biomarker for bladder cancer. PMID: 29512774
    2. The impact of AKT1 on glucocorticoid receptor (GR)-induced transcriptional activity in cooperation with phospho-serine/threonine-binding protein 14-3-3, was examined. PMID: 27717743
    3. 14-3-3 eta isoform colocalizes TDP-43 on the coarse granules in the anterior horn cells of patients with sporadic amyotrophic lateral sclerosis. PMID: 27256400
    4. Extracellular 14-3-3eta activates key signalling cascades and induces factors associated with the pathogenesis of rheumatoid arthritis PMID: 24751211
    5. This study identified YWHAH significantly associated loci with a biologically plausible role in schizophrenia. PMID: 24507884
    6. 14-3-3eta may be required for mitotic progression and may be considered as a novel anti-cancer strategy in combination with microtubule inhibitors. PMID: 22562251
    7. This is the first genetic association study of YWHAH with sporadic Creutzfeldt-Jakob disease populations. PMID: 21739144
    8. relocation of p33ING1b from the nucleus to the cytoplasm, where the protein is tethered by 14-3-3eta, participates in tumorigenesis and progression in HNSCC PMID: 21432775
    9. The 14-3-3 eta, beta, gamma and sigma isoforms were negatively expressed in meningioma PMID: 20388496
    10. phosphorylation of 14-3-3 binding site of myeloid leukemia factor 1 by MADM PMID: 12176995
    11. Data demonstrate that 14-3-3 proteins are colocalized with Lewy bodies in Parkinson disease, but there was no specific staining for the 14-3-3 eta subunit. PMID: 12480176
    12. gremlin 1 is overexpressed in human cancers and interacts with YWHAH protein PMID: 16545136
    13. The association of YWHAH with bipolar disorder in a large sample, consisting of 1211 subjects from 318 nuclear families including 554 affected offspring, was investigated. PMID: 19160447

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  • 蛋白家族：
    14-3-3 family
  • 組織特異性：
    Expressed mainly in the brain and present in other tissues albeit at lower levels.
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 12853

    OMIM: 113508

    KEGG: hsa:7533

    STRING: 9606.ENSP00000248975

    UniGene: Hs.226755



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