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SKA3 Antibody

  • 中文名稱：
    SKA3兔多克隆抗體
  • 貨號：
    CSB-PA816899LA01HU
  • 規(guī)格：
    ￥440
  • 圖片：
    • IHC image of CSB-PA816899LA01HU diluted at 1:300 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • IHC image of CSB-PA816899LA01HU diluted at 1:300 and staining in paraffin-embedded human melanoma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of Hela cells with CSB-PA816899LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

產品詳情

  • 產品名稱：
    Rabbit anti-Homo sapiens (Human) SKA3 Polyclonal antibody
  • Uniprot No.：
  • 基因名：
    SKA3
  • 別名：
    C13orf3 antibody; RAMA1 antibody; SKA3 antibody; SKA3_HUMAN antibody; Spindle and kinetochore associated complex subunit 3 antibody; Spindle and kinetochore-associated protein 3 antibody
  • 宿主：
    Rabbit
  • 反應種屬：
    Human
  • 免疫原：
    Recombinant Human Spindle and kinetochore-associated protein 3 protein (101-195AA)
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated

    本頁面中的產品，SKA3 Antibody (CSB-PA816899LA01HU)，的標記方式是Non-conjugated。對于SKA3 Antibody，我們還提供其他標記。見下表：

    可提供標記
    標記方式 貨號 產品名稱 應用
    HRP CSB-PA816899LB01HU SKA3 Antibody, HRP conjugated ELISA
    FITC CSB-PA816899LC01HU SKA3 Antibody, FITC conjugated
    Biotin CSB-PA816899LD01HU SKA3 Antibody, Biotin conjugated ELISA
  • 克隆類型：
    Polyclonal
  • 抗體亞型：
    IgG
  • 純化方式：
    >95%, Protein G purified
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, IHC, IF
  • 推薦稀釋比：
    Application Recommended Dilution
    IHC 1:200-1:500
    IF 1:50-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner. In the complex, it mediates the microtubule-stimulated oligomerization. Affinity for microtubules is synergistically enhanced in the presence of the ndc-80 complex and may allow the ndc-80 complex to track depolymerizing microtubules.
  • 基因功能參考文獻：
    1. decreased neuronal cell SKA complex genes' expression levels affect neuronal cell viability and neurite development PMID: 29268205
    2. Study shows that Cdk1 phosphorylates Ska3 to promote its direct binding to the Ndc80 complex (Ndc80C), a core outer kinetochore component, also show that this phosphorylation occurs specifically during mitosis and is required for the kinetochore localization of the Ska complex. PMID: 28479321
    3. this report provides clear evidence that overexpression of the AURKA, SKA3, and DSN1 genes strongly correlates with the progression of colorectal adenomas to colorectal cancer PMID: 27329586
    4. Kinetochores mature through Ska1/ska2/Ska3 complex recruitment and this is required for improved load-bearing capacity and silencing of the spindle assembly checkpoint. PMID: 28495837
    5. over-expression of GNL3 and SKA3 in the PC-3 human prostate cancer cell line decreased in vitro cell migration and invasion PMID: 26429724
    6. The structure of the Ska core complex is a W-shaped dimer of coiled coils, formed by intertwined interactions between Ska1, Ska2, and Ska3. PMID: 22483620
    7. Data suggest that Aurora B phosphorylation antagonizes the interaction between the Ska1-3 complex and the KMN network, thereby controlling Ska recruitment to KTs and stabilization of KT-MT attachments. PMID: 22371557
    8. Ska3 functions to coordinate checkpoint signaling from the microtubule binding sites within a kinetochore by laterally linking the individual binding sites. PMID: 19646878
    9. C13Orf3 (now termed Ska3) localizes to the spindle and kinetochore throughout mitosis and its depletion markedly delays anaphase transition. PMID: 19360002
    10. These data identify C13orf3 (Ska3) as an important factor for metaphase to anaphase progression and highlight the potential of combined RNAi screening and protein localisation analyses. PMID: 19387489
    11. RAMA1 is a novel kinetochore protein involved in kinetochore-microtubule attachment. PMID: 19549680

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  • 亞細胞定位：
    Cytoplasm, cytoskeleton, spindle. Chromosome, centromere, kinetochore.
  • 蛋白家族：
    SKA3 family
  • 數據庫鏈接：

    HGNC: 20262

    KEGG: hsa:221150

    STRING: 9606.ENSP00000319417

    UniGene: Hs.88523



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