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  4. PIH1D1 Antibody

PIH1D1 Antibody

  • 中文名稱：
    PIH1D1兔多克隆抗體
  • 貨號：
    CSB-PA889130LA01HU
  • 規格：
    ￥440
  • 圖片：
    • Immunohistochemistry of paraffin-embedded human placenta tissue using CSB-PA889130LA01HU at dilution of 1:100
    • Immunofluorescence staining of Hela cells cells with CSB-PA889130LA01HU at 1: 200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized tissue using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) .
    • Immunoprecipitating PIH1D1 in Hela whole cell lysate
      Lane 1: Rabbit control IgG (1ug) instead of CSB-PA889130LA01HU in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
      Lane 2: CSB-PA889130LA01HU (6ug) + Hela whole cell lysate (500ug)
      Lane 3: Hela whole cell lysate (10ug)
    • Western Blot
      Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, U87 whole cell lysate, K562 whole cell lysate, 293 whole cell lysate
      All lanes: PIH1D1 antibody at 1:2000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 33, 20, 22 kDa
      Observed band size: 39 kDa
  • 其他：

產品詳情

  • 產品名稱：
    Rabbit anti-Homo sapiens (Human) PIH1D1 Polyclonal antibody
  • Uniprot No.：
  • 基因名：
    PIH1D1
  • 別名：
    NOP17 antibody; Nucleolar protein 17 homolog antibody; PIH1 domain containing 1 antibody; PIH1 domain containing protein 1 antibody; PIH1 domain-containing protein 1 antibody; PIH1D1 antibody; PIHD1_HUMAN antibody
  • 宿主：
    Rabbit
  • 反應種屬：
    Human
  • 免疫原：
    Recombinant Human PIH1 domain-containing protein 1 protein (1-290AA)
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated

    本頁面中的產品，PIH1D1 Antibody (CSB-PA889130LA01HU)，的標記方式是Non-conjugated。對于PIH1D1 Antibody，我們還提供其他標記。見下表：

    可提供標記
    標記方式 貨號 產品名稱 應用
    HRP CSB-PA889130LB01HU PIH1D1 Antibody, HRP conjugated ELISA
    FITC CSB-PA889130LC01HU PIH1D1 Antibody, FITC conjugated
    Biotin CSB-PA889130LD01HU PIH1D1 Antibody, Biotin conjugated ELISA
  • 克隆類型：
    Polyclonal
  • 抗體亞型：
    IgG
  • 純化方式：
    >95%, Protein G purified
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB, IHC, IF, IP
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:20-1:200
    IF 1:50-1:500
    IP 1:200-1:2000
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    Involved in the assembly of C/D box small nucleolar ribonucleoprotein (snoRNP) particles. Recruits the SWI/SNF complex to the core promoter of rRNA genes and enhances pre-rRNA transcription. Mediates interaction of TELO2 with the R2TP complex which is necessary for the stability of MTOR and SMG1. Positively regulates the assembly and activity of the mTORC1 complex.
  • 基因功能參考文獻：
    1. We propose that protein complexes assembled by the R2TP complex are defined by phosphorylation of a specific motif and recognition by the PIH1D1 subunit PMID: 24656813
    2. PIH1D1 interacts with mTOR complex 1 and enhances ribosome RNA transcription. PMID: 24036451
    3. Human PIH1 domain-containing protein 1 (PIH1) interacts directly with histone H4 and recruits the Brg1-SWI/SNF complex via SNF5 to human rRNA genes. PMID: 22368283
    4. this study investigated the interaction between RPAP3 and PIH1D1. PMID: 23159623
    5. PIH1D1 may stabilize SNF5 by attenuating its proteasome degradation pathway. PMID: 20078948
    6. These results suggest that PIH1D1 may also function as a novel modulator of apoptosis pathway. PMID: 21078300
    7. Is part of an RNA polymerase II-associated complex with possible chaperone activity. PMID: 19450687

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  • 亞細胞定位：
    Nucleus.
  • 蛋白家族：
    PIH1 family
  • 組織特異性：
    Expressed at low levels in normal mammary epithelial cells (at protein level). Highest expression in lung, leukocyte and placenta. Expressed at lower levels in brain, prostate, colon, heart, small intestine, liver, ovary, pancreas, skeletal muscle, spleen
  • 數據庫鏈接：

    HGNC: 26075

    OMIM: 611480

    KEGG: hsa:55011

    STRING: 9606.ENSP00000262265

    UniGene: Hs.5245



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