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PAM Antibody

  • 中文名稱：
    PAM兔多克隆抗體
  • 貨號：
    CSB-PA017417LA01HU
  • 規格：
    ￥440
  • 圖片：
    • Western Blot
      Positive WB detected in: Hela whole cell lysate
      All lanes: PAM antibody at 5.6µg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 109, 97, 101, 99, 107 kDa
      Observed band size: 109 kDa
    • IHC image of CSB-PA017417LA01HU diluted at 1:200 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • IHC image of CSB-PA017417LA01HU diluted at 1:200 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of Hela cells with CSB-PA017417LA01HU at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

產品詳情

  • 產品名稱：
    Rabbit anti-Homo sapiens (Human) PAM Polyclonal antibody
  • Uniprot No.：
  • 基因名：
    PAM
  • 別名：
    AMD_HUMAN antibody; PAL antibody; PAM antibody; Pancreatic peptidylglycine alpha amidating monooxygenase antibody; Peptidyl alpha amidating enzyme antibody; Peptidyl alpha hydroxyglycine alpha amidating lyase antibody; Peptidyl-alpha-hydroxyglycine alpha-amidating lyase antibody; Peptidylamidoglycolate lyase antibody; Peptidylglycine 2 hydroxylase antibody; Peptidylglycine alpha amidating monooxygenase antibody; Peptidylglycine alpha hydroxylating monooxygenase antibody; PHM antibody
  • 宿主：
    Rabbit
  • 反應種屬：
    Human
  • 免疫原：
    Recombinant Human Peptidyl-glycine alpha-amidating monooxygenase protein (338-497AA)
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated

    本頁面中的產品，PAM Antibody (CSB-PA017417LA01HU)，的標記方式是Non-conjugated。對于PAM Antibody，我們還提供其他標記。見下表：

    可提供標記
    標記方式 貨號 產品名稱 應用
    HRP CSB-PA017417LB01HU PAM Antibody, HRP conjugated ELISA
    FITC CSB-PA017417LC01HU PAM Antibody, FITC conjugated
    Biotin CSB-PA017417LD01HU PAM Antibody, Biotin conjugated ELISA
  • 克隆類型：
    Polyclonal
  • 抗體亞型：
    IgG
  • 純化方式：
    >95%, Protein G purified
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB, IHC, IF
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:200-1:500
    IF 1:50-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    Bifunctional enzyme that catalyzes the post-translational modification of inactive peptidylglycine precursors to the corresponding bioactive alpha-amidated peptides, a terminal modification in biosynthesis of many neural and endocrine peptides. Alpha-amidation involves two sequential reactions, both of which are catalyzed by separate catalytic domains of the enzyme. The first step, catalyzed by peptidyl alpha-hydroxylating monoxygenase (PHM) domain, is the copper-, ascorbate-, and O2- dependent stereospecific hydroxylation (with S stereochemistry) at the alpha-carbon (C-alpha) of the C-terminal glycine of the peptidylglycine substrate. The second step, catalyzed by the peptidylglycine amidoglycolate lyase (PAL) domain, is the zinc-dependent cleavage of the N-C-alpha bond, producing the alpha-amidated peptide and glyoxylate. Similarly, catalyzes the two-step conversion of an N-fatty acylglycine to a primary fatty acid amide and glyoxylate.
  • 基因功能參考文獻：
    1. PAM single nucleotide polymorphism rs13175330 is associated with hypertension and insulin resistance in a Korean population. PMID: 29162152
    2. The ancient ability of PAM to localize to ciliary membranes, which release bioactive ectosomes, may be related to its ability to accumulate in intralumenal vesicles and exosomes PMID: 28377049
    3. Data suggest that His108 and a substrate molecule are involved in the reductive pathway while His172 and Tyr79 are important in the catalytic pathway in the copper-centered electron transfer catalyzed by peptidylglycine monooxygenase. PMID: 26982589
    4. PAM expression is increased in the secretory pathway of differentiated neurons. PMID: 26879543
    5. Oxygen Sensitivity of the Peptidylglycine alpha-Amidating Monooxygenase (PAM) in Neuroendocrine Cells PMID: 26296884
    6. Two missense variants in PAM, encoding p.Asp563Gly (frequency of 4.98%) and p.Ser539Trp (frequency of 0.65%), confer moderately higher risk of type 2 diabetes (OR = 1.23, P = 3.9 x 10(-10) and OR = 1.47, P = 1.7 x 10(-5), respectively) PMID: 24464100
    7. Detail the production of the catalytic core of human peptidylglycine alpha-hydroxylating monooxygenase (hPHMcc) in Escherichia coli possessing a N-terminal fusion to thioredoxin (Trx). PMID: 22554821
    8. Data indicate that catalytic inactivation of PHM caused by pH changes is accompanied by structural change between two states of the protein involving strong Cu-S interaction that does not involve M314. PMID: 22080626
    9. nuclear retention of PAM mRNA is lost upon expressing the La proteins that lack a conserved nuclear retention element, suggesting a direct association between PAM mRNA and La protein in vivo PMID: 16107699

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  • 亞細胞定位：
    Cytoplasmic vesicle, secretory vesicle membrane; Single-pass membrane protein.; [Isoform 1]: Membrane; Single-pass type I membrane protein.; [Isoform 2]: Membrane; Single-pass type I membrane protein.; [Isoform 3]: Secreted. Note=Secreted from secretory granules.; [Isoform 4]: Secreted. Note=Secreted from secretory granules.
  • 蛋白家族：
    Peptidyl-alpha-hydroxyglycine alpha-amidating lyase family; Copper type II ascorbate-dependent monooxygenase family
  • 數據庫鏈接：

    HGNC: 8596

    OMIM: 170270

    KEGG: hsa:5066

    STRING: 9606.ENSP00000306100

    UniGene: Hs.369430



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