在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

  1. Home
  2. 抗體
  3. 多克隆抗體
  4. PAM Antibody

PAM Antibody

  • 中文名稱：
    PAM兔多克隆抗體
  • 貨號：
    CSB-PA017417LA01HU
  • 規格：
    ￥440
  • 圖片：
    • Western Blot
      Positive WB detected in: Hela whole cell lysate
      All lanes: PAM antibody at 5.6µg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 109, 97, 101, 99, 107 kDa
      Observed band size: 109 kDa
    • IHC image of CSB-PA017417LA01HU diluted at 1:200 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • IHC image of CSB-PA017417LA01HU diluted at 1:200 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of Hela cells with CSB-PA017417LA01HU at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

產品詳情

  • 產品名稱：
    Rabbit anti-Homo sapiens (Human) PAM Polyclonal antibody
  • Uniprot No.：
  • 基因名：
    PAM
  • 別名：
    AMD_HUMAN antibody; PAL antibody; PAM antibody; Pancreatic peptidylglycine alpha amidating monooxygenase antibody; Peptidyl alpha amidating enzyme antibody; Peptidyl alpha hydroxyglycine alpha amidating lyase antibody; Peptidyl-alpha-hydroxyglycine alpha-amidating lyase antibody; Peptidylamidoglycolate lyase antibody; Peptidylglycine 2 hydroxylase antibody; Peptidylglycine alpha amidating monooxygenase antibody; Peptidylglycine alpha hydroxylating monooxygenase antibody; PHM antibody
  • 宿主：
    Rabbit
  • 反應種屬：
    Human
  • 免疫原：
    Recombinant Human Peptidyl-glycine alpha-amidating monooxygenase protein (338-497AA)
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated

    本頁面中的產品，PAM Antibody (CSB-PA017417LA01HU)，的標記方式是Non-conjugated。對于PAM Antibody，我們還提供其他標記。見下表：

    可提供標記
    標記方式 貨號 產品名稱 應用
    HRP CSB-PA017417LB01HU PAM Antibody, HRP conjugated ELISA
    FITC CSB-PA017417LC01HU PAM Antibody, FITC conjugated
    Biotin CSB-PA017417LD01HU PAM Antibody, Biotin conjugated ELISA
  • 克隆類型：
    Polyclonal
  • 抗體亞型：
    IgG
  • 純化方式：
    >95%, Protein G purified
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB, IHC, IF
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:200-1:500
    IF 1:50-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    Bifunctional enzyme that catalyzes the post-translational modification of inactive peptidylglycine precursors to the corresponding bioactive alpha-amidated peptides, a terminal modification in biosynthesis of many neural and endocrine peptides. Alpha-amidation involves two sequential reactions, both of which are catalyzed by separate catalytic domains of the enzyme. The first step, catalyzed by peptidyl alpha-hydroxylating monoxygenase (PHM) domain, is the copper-, ascorbate-, and O2- dependent stereospecific hydroxylation (with S stereochemistry) at the alpha-carbon (C-alpha) of the C-terminal glycine of the peptidylglycine substrate. The second step, catalyzed by the peptidylglycine amidoglycolate lyase (PAL) domain, is the zinc-dependent cleavage of the N-C-alpha bond, producing the alpha-amidated peptide and glyoxylate. Similarly, catalyzes the two-step conversion of an N-fatty acylglycine to a primary fatty acid amide and glyoxylate.
  • 基因功能參考文獻：
    1. PAM single nucleotide polymorphism rs13175330 is associated with hypertension and insulin resistance in a Korean population. PMID: 29162152
    2. The ancient ability of PAM to localize to ciliary membranes, which release bioactive ectosomes, may be related to its ability to accumulate in intralumenal vesicles and exosomes PMID: 28377049
    3. Data suggest that His108 and a substrate molecule are involved in the reductive pathway while His172 and Tyr79 are important in the catalytic pathway in the copper-centered electron transfer catalyzed by peptidylglycine monooxygenase. PMID: 26982589
    4. PAM expression is increased in the secretory pathway of differentiated neurons. PMID: 26879543
    5. Oxygen Sensitivity of the Peptidylglycine alpha-Amidating Monooxygenase (PAM) in Neuroendocrine Cells PMID: 26296884
    6. Two missense variants in PAM, encoding p.Asp563Gly (frequency of 4.98%) and p.Ser539Trp (frequency of 0.65%), confer moderately higher risk of type 2 diabetes (OR = 1.23, P = 3.9 x 10(-10) and OR = 1.47, P = 1.7 x 10(-5), respectively) PMID: 24464100
    7. Detail the production of the catalytic core of human peptidylglycine alpha-hydroxylating monooxygenase (hPHMcc) in Escherichia coli possessing a N-terminal fusion to thioredoxin (Trx). PMID: 22554821
    8. Data indicate that catalytic inactivation of PHM caused by pH changes is accompanied by structural change between two states of the protein involving strong Cu-S interaction that does not involve M314. PMID: 22080626
    9. nuclear retention of PAM mRNA is lost upon expressing the La proteins that lack a conserved nuclear retention element, suggesting a direct association between PAM mRNA and La protein in vivo PMID: 16107699

    顯示更多

    收起更多

  • 亞細胞定位：
    Cytoplasmic vesicle, secretory vesicle membrane; Single-pass membrane protein.; [Isoform 1]: Membrane; Single-pass type I membrane protein.; [Isoform 2]: Membrane; Single-pass type I membrane protein.; [Isoform 3]: Secreted. Note=Secreted from secretory granules.; [Isoform 4]: Secreted. Note=Secreted from secretory granules.
  • 蛋白家族：
    Peptidyl-alpha-hydroxyglycine alpha-amidating lyase family; Copper type II ascorbate-dependent monooxygenase family
  • 數據庫鏈接：

    HGNC: 8596

    OMIM: 170270

    KEGG: hsa:5066

    STRING: 9606.ENSP00000306100

    UniGene: Hs.369430



主站蜘蛛池模板： 亚洲2020天天堂在线观看 | 九色porny丨自拍视频| 中文字幕亚韩| 男人av无码天堂| 亚洲精品国产精品国产自2022| 国产老太睡小伙子视频| 中文字幕一精品亚洲无线一区 | 亚洲中文无码a∨在线观看| 亚洲成在线aⅴ免费视频| 国语精品自产拍在线观看网站| 香港aa三级久久三级| 韩国精品一区二区三区四区| 丰满少妇大叫太大太粗| 亚洲人成日韩中文字幕无卡| 污污内射久久一区二区欧美日韩| 激情五月色综合国产精品| 97国产超碰一区二区三区| 久久精品成人亚洲另类欧美| 国产精品无码免费播放| 在线亚洲精品国产一区麻豆| 精品国产中文字幕在线视频| 免费观看美女裸体网站| 国精产品999国精产| 久久久久精品无码一区二区三区| 亚洲全部无码中文字幕| 國产一二三内射在线看片| 永久免费的av片在线电影网| 国产精品无码一区二区牛牛| 久久婷婷成人综合色综合 | 高中生粉嫩无套第一次| 国产精品人人妻人人爽人人牛| 国产精品1区2区3区在线观看| 国产在线看片免费观看| 欧美另类精品xxxx| 久久精品国产大片免费观看| 免费国产在线一区二区| 亚洲s久久久久一区二区| 污污污污污污www网站免费| 青青青国产精品国产精品美女| 亚洲五月综合缴情在线观看| 免费国偷自产拍精品视频|