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  4. PAFAH2 Antibody

PAFAH2 Antibody

  • 中文名稱：
    PAFAH2兔多克隆抗體
  • 貨號：
    CSB-PA857448LA01HU
  • 規格：
    ￥440
  • 圖片：
    • IHC image of CSB-PA857448LA01HU diluted at 1:300 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of HepG2 cells with CSB-PA857448LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

產品詳情

  • 產品名稱：
    Rabbit anti-Homo sapiens (Human) PAFAH2 Polyclonal antibody
  • Uniprot No.：
  • 基因名：
    PAFAH2
  • 別名：
    PAFAH2Platelet-activating factor acetylhydrolase 2 antibody; cytoplasmic antibody; EC 3.1.1.47 antibody; Serine-dependent phospholipase A2 antibody; SD-PLA2 antibody; hSD-PLA2 antibody
  • 宿主：
    Rabbit
  • 反應種屬：
    Human
  • 免疫原：
    Recombinant Human Platelet-activating factor acetylhydrolase 2, cytoplasmic protein (1-206AA)
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated

    本頁面中的產品，PAFAH2 Antibody (CSB-PA857448LA01HU)，的標記方式是Non-conjugated。對于PAFAH2 Antibody，我們還提供其他標記。見下表：

    可提供標記
    標記方式 貨號 產品名稱 應用
    HRP CSB-PA857448LB01HU PAFAH2 Antibody, HRP conjugated ELISA
    FITC CSB-PA857448LC01HU PAFAH2 Antibody, FITC conjugated
    Biotin CSB-PA857448LD01HU PAFAH2 Antibody, Biotin conjugated ELISA
  • 克隆類型：
    Polyclonal
  • 抗體亞型：
    IgG
  • 純化方式：
    >95%, Protein G purified
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, IHC, IF
  • 推薦稀釋比：
    Application Recommended Dilution
    IHC 1:200-1:500
    IF 1:50-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    Catalyzes the hydrolyze of the acetyl group at the sn-2 position of platelet-activating factor (PAF) and its analogs, leading to their inactivation. Hydrolyzes propionyl and butyroyl moieties approximately half as effectively as PAF. Also catalyzes transacetylation of the acetyl group from platelet-activating factor (PAF) to lysoplasmalogen and to sphingosine, producing plasmalogen analogs of PAF and N-acetylsphingosine (C2-ceramide) respectively. Has a marked selectivity for phospholipids with short acyl chains at the sn-2 position.
  • 基因功能參考文獻：
    1. Data show that the activity of plasma platelet activating factor acetylhydrolase (PAF-AH) increases in parallel with diabetic retinopathy (DR) severity. PMID: 26791393
    2. The oligomeric state of PAFAH-II drives functional protein trafficking. PAFAH-II localization to the membrane is critical for substrate acquisition and effective oxidative stress protection. PMID: 25707358
    3. presence of platelet-activating factor acetylhydrolase II in a restricted pattern in human skin; it is involved in protecting this organ against oxidative stress through the degradation of oxidatively modified bioactive phospholipids. PMID: 12406338
    4. Lp-PLA2 was higher in those with more extensive Coronary artery disease PMID: 17157859
    5. PAF-AH II exerts strong neuroprotective effects against ischemic injury: the neurological deficit scores, cerebral edema index, and relative infarction volume are all significantly lower in PAFAH2 transgenic mice than in wild-type mice. PMID: 17272759
    6. examines substrate specificity for intracellular and plasma PAF-AHs PMID: 11294621

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  • 亞細胞定位：
    Cytoplasm. Membrane; Lipid-anchor. Endoplasmic reticulum membrane; Lipid-anchor.
  • 蛋白家族：
    Serine esterase family
  • 組織特異性：
    Broadly expressed in different tissues, but high in B- and T-lymphocytes. In brain, expression is restricted to amygdala and frontal cortex.
  • 數據庫鏈接：

    HGNC: 8579

    OMIM: 602344

    KEGG: hsa:5051

    STRING: 9606.ENSP00000363400

    UniGene: Hs.477083



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