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NIT1 Antibody

  • 中文名稱：
    NIT1兔多克隆抗體
  • 貨號(hào)：
    CSB-PA768228LA01HU
  • 規(guī)格：
    ￥440
  • 圖片：
    • Western Blot
      Positive WB detected in: Mouse brain tissue, Rat brain tissue
      All lanes: NIT1 antibody at 3µg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 36, 32, 41, 35, 27 kDa
      Observed band size: 36 kDa
    • IHC image of CSB-PA768228LA01HU diluted at 1:1200 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of HepG2 cells with CSB-PA768228LA01HU at 1:400, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品名稱：
    Rabbit anti-Homo sapiens (Human) NIT1 Polyclonal antibody
  • Uniprot No.：
  • 基因名：
    NIT1
  • 別名：
    MGC57670 antibody; NIT1 antibody; NIT1_HUMAN antibody; Nitrilase 1 antibody; Nitrilase homolog 1 antibody
  • 宿主：
    Rabbit
  • 反應(yīng)種屬：
    Human, Mouse, Rat
  • 免疫原：
    Recombinant Human Deaminated glutathione amidase protein (1-243AA)
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated

    本頁(yè)面中的產(chǎn)品，NIT1 Antibody (CSB-PA768228LA01HU)，的標(biāo)記方式是Non-conjugated。對(duì)于NIT1 Antibody，我們還提供其他標(biāo)記。見(jiàn)下表：

    可提供標(biāo)記
    標(biāo)記方式 貨號(hào) 產(chǎn)品名稱 應(yīng)用
    HRP CSB-PA768228LB01HU NIT1 Antibody, HRP conjugated ELISA
    FITC CSB-PA768228LC01HU NIT1 Antibody, FITC conjugated
    Biotin CSB-PA768228LD01HU NIT1 Antibody, Biotin conjugated ELISA
  • 克隆類型：
    Polyclonal
  • 抗體亞型：
    IgG
  • 純化方式：
    >95%, Protein G purified
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, WB, IHC, IF
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:1000-1:2000
    IF 1:200-1:500
  • Protocols：
  • 儲(chǔ)存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Catalyzes the hydrolysis of the amide bond in N-(4-oxoglutarate)-L-cysteinylglycine (deaminated glutathione), a metabolite repair reaction to dispose of the harmful deaminated glutathione. Plays a role in cell growth and apoptosis: loss of expression promotes cell growth, resistance to DNA damage stress and increased incidence to NMBA-induced tumors. Has tumor suppressor properties that enhances the apoptotic responsiveness in cancer cells; this effect is additive to the tumor suppressor activity of FHIT. It is also a negative regulator of primary T-cells.
  • 基因功能參考文獻(xiàn)：
    1. Data show that cisplatin response was enhanced in human lung cancer cells when Nit1 was knocked down and mice Nit1-/-:KrasG12D/+ tumors showed increased sensitivity to cisplatin in vivo. PMID: 26967383
    2. Human NIT1/2 are not involved in the metabolism of vildagliptin. PMID: 25008847
    3. Nit1 and Fhit share tumor suppressor signaling pathways, while localization of the NIT1 gene at a stable chromosome site explains the paucity of gene alterations and in frequent loss of expression of the NIT1 gene in human malignancies. PMID: 16864578
  • 亞細(xì)胞定位：
    [Isoform 2]: Mitochondrion.; [Isoform 1]: Cytoplasm.
  • 蛋白家族：
    Carbon-nitrogen hydrolase superfamily, NIT1/NIT2 family
  • 組織特異性：
    Detected in heart, brain, placenta, liver, skeletal muscle, kidney and pancreas.
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 7828

    OMIM: 604618

    KEGG: hsa:4817

    STRING: 9606.ENSP00000356988

    UniGene: Hs.146406



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