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NELFB Antibody

  • 中文名稱：
    NELFB兔多克隆抗體
  • 貨號：
    CSB-PA03765A0Rb
  • 規格：
    ￥440
  • 圖片：
    • Western Blot
      All lanes: NELFB antibody at 2µg/ml
      Lane 1: NIH/3T3 whole cell lysate
      Lane 2: HL60 whole cell lysate
      Secondary
      Goat polyclonal to rabbit IgG at 1/15000 dilution
      Predicted band size: 66 kDa
      Observed band size: 66 kDa
    • Immunohistochemistry of paraffin-embedded human endometrial cancer using CSB-PA03765A0Rb at dilution of 1:100
    • Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA03765A0Rb at dilution of 1:100
    • Chromatin Immunoprecipitation Hela (1.1*106) were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4µg anti-NELFB or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers (CSB-PP03765HU) against the TFF1 promoter.
  • 其他：

產品詳情

  • 產品描述：

    The NELFB antibody (CSB-PA03765A0Rb) is produced in the rabbit with a peptide corresponding to amino acids 8-199 of the human NELFB immunized. This polyclonal antibody occurs as an unconjugated IgG. Its purity is 95%+ using protein G purified. It is reactive with human and mouse samples. The NELFB protein is a subunit of NELF, which is known to enforce promoter-proximal pausing of RNA polymerase II. The functional cardiac deterioration in NELFB knockout mice strongly indicates an important role of NELFB in the maintenance of normal cardiac contractility under stressed conditions. This anti-NELFB antibody has been quality-verified in ELISA, WB, IHC, and ChIP applications.

  • 產品名稱：
    Rabbit anti-Homo sapiens (Human) NELFB Polyclonal antibody
  • Uniprot No.：
  • 基因名：
    NELFB
  • 別名：
    NELFB antibody; COBRA1 antibody; KIAA1182 antibody; Negative elongation factor B antibody; NELF-B antibody; Cofactor of BRCA1 antibody
  • 宿主：
    Rabbit
  • 反應種屬：
    Human, Mouse
  • 免疫原：
    Recombinant Human Negative elongation factor B protein (8-199AA)
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated

    本頁面中的產品，NELFB Antibody (CSB-PA03765A0Rb)，的標記方式是Non-conjugated。對于NELFB Antibody，我們還提供其他標記。見下表：

    可提供標記
    標記方式 貨號 產品名稱 應用
    HRP CSB-PA03765B0Rb NELFB Antibody, HRP conjugated ELISA
    FITC CSB-PA03765C0Rb NELFB Antibody, FITC conjugated
    Biotin CSB-PA03765D0Rb NELFB Antibody, Biotin conjugated ELISA
  • 克隆類型：
    Polyclonal
  • 抗體亞型：
    IgG
  • 純化方式：
    >95%, Protein G purified
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB, IHC, ChIP
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:1000-1:5000
    IHC 1:20-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. May be able to induce chromatin unfolding. Essential for early embryogenesis; plays an important role in maintaining the undifferentiated state of embryonic stem cells (ESCs) by preventing unscheduled expression of developmental genes. Plays a key role in establishing the responsiveness of stem cells to developmental cues; facilitates plasticity and cell fate commitment in ESCs by establishing the appropriate expression level of signaling molecules. Supports the transcription of genes involved in energy metabolism in cardiomyocytes; facilitates the association of transcription initiation factors with the promoters of the metabolism-related genes.; (Microbial infection) The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II. In vitro, binds weakly to the HIV-1 TAR RNA which is located in the long terminal repeat (LTR) of HIV-1.
  • 基因功能參考文獻：
    1. BRCA1 Interacting Protein COBRA1 Facilitates Adaptation to Castrate-Resistant Growth Conditions. PMID: 30036938
    2. A positively charged face of NELF-AC is involved in RNA binding, whereas the opposite face of the NELF-AC subcomplex binds NELF-B. NELF-B is predicted to form a HEAT repeat fold, also binds RNA in vivo, and anchors the subunit NELF-E, which is confirmed to bind RNA in vivo. PMID: 27282391
    3. High COBRA1 expression is associated with hepatocellular carcinoma. PMID: 28112367
    4. both human and mouse NELF-B proteins are translated from a non-AUG codon upstream of the annotated AUG. PMID: 26010750
    5. These studies allow us to position the actions of two new modulators of GR-regulated transactivation, NELF-A and NELF-B, relative to other factors in the overall gene induction sequence. PMID: 24097989
    6. data show that NELF subunits exhibit highly specific subcellular localizations, such as in NELF bodies or in midbodies, and some shuttle actively between the nucleus and cytoplasm; loss of NELF from cells can lead to enlarged and/or multiple nuclei PMID: 19245807
    7. NELF-C and NELF-D are highly related or identical to the protein called TH1, of unknown function. NELF-B and NELF-C or NELF-D are integral subunits that bring NELF-A and NELF-E together. [NELF-B] [NELF-C] PMID: 12612062
    8. differentially expressed in breast cancer cell lines, antibody to it may be a useful tool to investigate its functions PMID: 15185750
    9. COBRA1 may directly modulate AP-1 pathway and, therefore, may play important roles in cell proliferation, differentiation, apoptosis, and oncogenesis. PMID: 15530430
    10. COBRA1 can negatively regulate the activator protein-1 (AP-1) complex at the TFF1 promoter and thus down-regulate TFF1 expression in gastric cancer cell lines. PMID: 16452188
    11. Decreasing NELF also correlated with displacement of a positioned nucleosome and increased acetylation of histone H4, suggesting coupling of transcription elongation and chromatin remodeling PMID: 17442680
    12. Negative elongation factor (NELF) is a four subunit transcription factor. Our results point to a surprising role of NELF in the 3' end processing of histone mRNAs and suggest that NELF is a new factor that coordinates mRNA processing in transcription. PMID: 17499042
    13. COBRA1 may coordinate multiple steps in ligand-dependent gene expression, which in turn ensures both the quantity and quality of hormone-stimulated gene products PMID: 17659869
    14. a lack of COBRA1 expression in breast carcinoma may serve as a useful indicator for poor prognosis. PMID: 17910036
    15. COBRA1 and BRCA1 may engage in common gene regulatory pathways to suppress breast cancer progression. PMID: 18071589

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  • 亞細胞定位：
    Nucleus.
  • 蛋白家族：
    NELF-B family
  • 組織特異性：
    Widely expressed. Expressed in heart, brain, lung, placenta, liver, skeletal muscle, kidney and pancreas.
  • 數據庫鏈接：

    HGNC: 24324

    OMIM: 611180

    KEGG: hsa:25920

    STRING: 9606.ENSP00000339495

    UniGene: Hs.521809



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