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NDI1 Antibody

  • 中文名稱:
    NDI1 Antibody
  • 貨號:
    CSB-PA783368XA01SVG
  • 規格:
    ¥880
  • 圖片:
    • Western Blot
      Positive WB detected in: recombinant protein
      All lanes:NDI1 Antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/20000 dilution
      Predicted band size: 33.5 kDa
      Observed band size: 34 kDa
  • 其他:

產品詳情

  • 產品名稱:
    Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) NDI1 Polyclonal antibody
  • Uniprot No.:
  • 基因名:
    NDI1
  • 別名:
    Rotenone-insensitive NADH-ubiquinone oxidoreductase, mitochondrial (EC 1.6.5.9) (Internal NADH dehydrogenase) (NADH:ubiquinone reductase (non-electrogenic)) NDI1 YML120C YM7056.06C
  • 宿主:
    Rabbit
  • 反應種屬:
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 免疫原:
    Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) NDI1 protein (27-270aa)
  • 免疫原種屬:
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 標記方式:
    Non-conjugated
  • 克隆類型:
    Polyclonal
  • 抗體亞型:
    IgG
  • 純化方式:
    Antigen Affinity Purified
  • 濃度:
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液:
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 產品提供形式:
    Liquid
  • 應用范圍:
    ELISA, WB
  • 推薦稀釋比:
    Application Recommended Dilution
    WB 1:500-1:5000
  • Protocols:
  • 儲存條件:
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期:
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途:
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能:
    Catalyzes the oxidation of NADH generated inside the Mitochondrion.
  • 基因功能參考文獻:
    1. Ubiquinone binding site of yeast NADH dehydrogenase Ndi1 has been described. PMID: 29402945
    2. Ndi1 associations took place with complexes III and IV suggest the formation of respirasome-like structures. PMID: 26173916
    3. Ndi1 assimilates electrons to the electron transport chain and initiates the respiration process to make ATP, whereas under stresses, it cleaves the toxicity-sequestering N-terminal cap, is released from the mitochondria, and becomes a cell killer. PMID: 22993213
    4. The structure of the yeast NADH dehydrogenase (Ndi1) reveals overlapping binding sites for water- and lipid-soluble substrates PMID: 22949654
    5. Our data indicate a central role for Complex I dysfunction in pink1-associated defects, and our genetic analyses with heterologous ETC enzymes PMID: 22242018
    6. Perfusion of TAT-Ndi1 before 30 min no-flow ischemia and up to 2 hr reperfusion suppressed ROS production and preserved ATP stores. Importantly, TAT-Ndi1 infused before ischemia reduced infarct size by 62%. PMID: 21339825
    7. the Ndi1 reaction proceeds through a ternary complex (not a ping-pong) mechanism. The bound UQ keeps oxygen away from the reduced flavin. PMID: 21220430
    8. Yeast NDI1 expression in Drosophila mitigated the aging associated decline in respiratory capacity and the accompanying increase in mitochondrial reactive oxygen species production, and resulted in decreased accumulation of markers of oxidative damage. PMID: 20435911
    9. report that expression of Ndi1 in fly mitochondria leads to an increase in NADH-ubiquinone oxidoreductase activity, oxygen consumption, and ATP levels. PMID: 20089120
    10. identify the ubiquinone binding site in Ndi1 PMID: 20192260
    11. Disruption of NDI1 decreases ROS production and elongates the chronological life span of yeast, accompanied by the loss of survival fitness. PMID: 16436509
    12. Oxidative stress was significantly decreased when the cells were transduced with NDI1. Furthermore, mitochondria from the NDI1-transduced cells showed a suppressed rate of ROS formation by the complex I inhibitors PMID: 17055488
    13. data suggest that Ndi1 bears at least two distinct Q sites: one for bound Q and the other for catalytic Q. PMID: 17200125
    14. NDI1 enzyme can improve the oxidative phosphorylation capacity in cells carrying the G11778A mutation and protect the cells from oxidative stress and cell death. PMID: 17320357

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  • 亞細胞定位:
    Mitochondrion inner membrane; Peripheral membrane protein; Matrix side. Note=Bound to the mitochondrial inner membrane facing the matrix site.
  • 蛋白家族:
    NADH dehydrogenase family
  • 數據庫鏈接:

    KEGG: sce:YML120C

    STRING: 4932.YML120C



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