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NANS Antibody

  • 中文名稱：
    NANS兔多克隆抗體
  • 貨號：
    CSB-PA885699LA01HU
  • 規(guī)格：
    ￥440
  • 圖片：
    • Western Blot
      Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate
      All lanes: NANS antibody at 5.5μg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 41 kDa
      Observed band size: 41 kDa
    • Immunofluorescence staining of HepG2 cells with CSB-PA885699LA01HU at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • IHC image of CSB-PA885699LA01HU diluted at 1:200 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • IHC image of CSB-PA885699LA01HU diluted at 1:200 and staining in paraffin-embedded human gastric cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品名稱：
    Rabbit anti-Homo sapiens (Human) NANS Polyclonal antibody
  • Uniprot No.：
  • 基因名：
    NANS
  • 別名：
    EC 2.5.1.56 antibody; EC 2.5.1.57 antibody; N acetylneuraminate 9 phosphate synthase antibody; N acetylneuraminate synthase antibody; N acetylneuraminic acid phosphate synthase antibody; N acetylneuraminic acid synthase antibody; N-acetylneuraminate synthase antibody; N-acetylneuraminate-9-phosphate synthase antibody; N-acetylneuraminic acid phosphate synthase antibody; N-acetylneuraminic acid synthase antibody; NANS antibody; OTTHUMP00000021769 antibody; SAS antibody; Sialic acid phosphate synthase antibody; Sialic acid synthase antibody; SIAS_HUMAN antibody
  • 宿主：
    Rabbit
  • 反應種屬：
    Human
  • 免疫原：
    Recombinant Human Sialic acid synthase protein (132-259AA)
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated

    本頁面中的產(chǎn)品，NANS Antibody (CSB-PA885699LA01HU)，的標記方式是Non-conjugated。對于NANS Antibody，我們還提供其他標記。見下表：

    可提供標記
    標記方式 貨號 產(chǎn)品名稱 應用
    HRP CSB-PA885699LB01HU NANS Antibody, HRP conjugated ELISA
    FITC CSB-PA885699LC01HU NANS Antibody, FITC conjugated
    Biotin CSB-PA885699LD01HU NANS Antibody, Biotin conjugated ELISA
  • 克隆類型：
    Polyclonal
  • 抗體亞型：
    IgG
  • 純化方式：
    >95%, Protein G purified
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 產(chǎn)品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB, IHC, IF
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:200-1:500
    IF 1:50-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

靶點詳情

  • 功能：
    Produces N-acetylneuraminic acid (Neu5Ac) and 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN). Can also use N-acetylmannosamine 6-phosphate and mannose 6-phosphate as substrates to generate phosphorylated forms of Neu5Ac and KDN, respectively.
  • 基因功能參考文獻：
    1. NANS deficiency is associated with defect in brain and skeletal development. PMID: 27213289
    2. sialic acid synthases kinetics and preliminary mutagenesis experiments reveal the importance of C-terminal antifreeze protein domain PMID: 16274664
    3. The structure of the C-terminal antifreeze-like domain of human sialic acid synthase was determined. The structure comprises one alpha- and two single-turn 3(10)-helices and two beta-strands, and is similar to those of the type III antifreeze proteins. PMID: 16597820
  • 相關(guān)疾病：
    Spondyloepimetaphyseal dysplasia, Genevieve type (SEMDG)
  • 組織特異性：
    Ubiquitous.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 19237

    OMIM: 605202

    KEGG: hsa:54187

    STRING: 9606.ENSP00000210444

    UniGene: Hs.522310



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