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NADK2 Antibody

  • 中文名稱：
    NADK2兔多克隆抗體
  • 貨號：
    CSB-PA676825LA01HU
  • 規(guī)格：
    ￥440
  • 圖片：
    • Western Blot
      Positive WB detected in: 293 whole cell lysate, HL60 whole cell lysate, Rat liver tissue, Mouse kidney tissue, Mouse liver tissue
      All lanes: NADK2 antibody at 4µg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 50, 46, 32 kDa
      Observed band size: 50, 46 kDa
    • IHC image of CSB-PA676825LA01HU diluted at 1:500 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • IHC image of CSB-PA676825LA01HU diluted at 1:500 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品名稱：
    Rabbit anti-Homo sapiens (Human) NADK2 Polyclonal antibody
  • Uniprot No.：
  • 基因名：
    NADK2
  • 別名：
    C5orf33 antibody; CE033_HUMAN antibody; Chromosome 5 open reading frame 33 antibody; FLJ30596 antibody; Hypothetical protein LOC133686 antibody; MGC43298 antibody; NADKD1 antibody; OTTHUMP00000161732 antibody; UPF0465 protein C5orf33 antibody
  • 宿主：
    Rabbit
  • 反應(yīng)種屬：
    Human, Rat, Mouse
  • 免疫原：
    Recombinant Human NAD kinase 2, mitochondrial protein (16-156AA)
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated

    本頁面中的產(chǎn)品，NADK2 Antibody (CSB-PA676825LA01HU)，的標(biāo)記方式是Non-conjugated。對于NADK2 Antibody，我們還提供其他標(biāo)記。見下表：

    可提供標(biāo)記
    標(biāo)記方式 貨號 產(chǎn)品名稱 應(yīng)用
    HRP CSB-PA676825LB01HU NADK2 Antibody, HRP conjugated ELISA
    FITC CSB-PA676825LC01HU NADK2 Antibody, FITC conjugated
    Biotin CSB-PA676825LD01HU NADK2 Antibody, Biotin conjugated ELISA
  • 克隆類型：
    Polyclonal
  • 抗體亞型：
    IgG
  • 純化方式：
    >95%, Protein G purified
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, WB, IHC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:500-1:1000
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能：
    Mitochondrial NAD(+) kinase that phosphorylates NAD(+) to yield NADP(+). Can use both ATP or inorganic polyphosphate as the phosphoryl donor. Also has weak NADH kinase activity in vitro; however NADH kinase activity is much weaker than the NAD(+) kinase activity and may not be relevant in vivo.
  • 基因功能參考文獻(xiàn)：
    1. The use of whole exome sequencing has allowed us to identify a new homozygous mutation in NADK2, leading to a premature stop codon and absence of protein. To our knowledge this is the second reported individual with mutations in NADK2. PMID: 27940755
    2. An autosomal recessive defect in NADK2 causes mitochondrial NADPH deficiency with secondary deficiencies in several mitochondrial processes including polyunsaturated fatty acid oxidation and lysine degradation. PMID: 24847004
    3. exhibits NAD kinase activity, utilizing ATP or inorganic polyphosphate, and is localized in mitochondria of HEK293A cells PMID: 23212377
    4. MNADK is localized to mitochondria in Hep G2 cells, and a recombinant MNADK showed NAD kinase activity. MNADK is abundant in mitochondrion-rich tissues, such as liver. MNADK expression was suppressed in human liver tumors. Identification of MNADK immediately suggests a model in which NADK and MNADK are responsible for de novo synthesis of NADP(+) in cytosol and mitochondria, respectively. PMID: 23616928
  • 相關(guān)疾病：
    2,4-dienoyl-CoA reductase deficiency (DECRD)
  • 亞細(xì)胞定位：
    Mitochondrion.
  • 蛋白家族：
    NAD kinase family
  • 組織特異性：
    Widely expressed.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 26404

    OMIM: 615787

    KEGG: hsa:133686

    STRING: 9606.ENSP00000371362

    UniGene: Hs.81907



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