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  4. Mono-methyl-HIST1H2BC (K12) Antibody

Mono-methyl-HIST1H2BC (K12) Antibody

  • 中文名稱：
    Mono-methyl-HIST1H2BC (K12)兔多克隆抗體
  • 貨號：
    CSB-PA010403OA12me1HU
  • 規格：
    ￥660
  • 圖片：
    • Western Blot
      Positive WB detected in: A549 whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate
      All lanes: HIST1H2BC antibody at 1:100
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 14 kDa
      Observed band size: 14 kDa
    • Immunocytochemistry analysis of CSB-PA010403OA12me1HU diluted at 1:30 and staining in Hela cells performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Chromatin Immunoprecipitation Hela (4*106) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-HIST1H2BC (CSB-PA010403OA12me1HU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
  • 其他：

產品詳情

  • 產品名稱：
    Rabbit anti-Homo sapiens (Human) HIST1H2BC Polyclonal antibody
  • Uniprot No.：
  • 基因名：
    HIST1H2BC
  • 別名：
    H2BC4 antibody; H2BFL antibody; HIST1H2BC; antibody; H2BC6 antibody; H2BFH antibody; HIST1H2BE; antibody; H2BC7 antibody; H2BFG antibody; HIST1H2BF; antibody; H2BC8 antibody; H2BFA antibody; HIST1H2BG; antibody; H2BC10 antibody; H2BFK antibody; HIST1H2BIHistone H2B type 1-C/E/F/G/I antibody; Histone H2B.1 A antibody; Histone H2B.a antibody; H2B/a antibody; Histone H2B.g antibody; H2B/g antibody; Histone H2B.h antibody; H2B/h antibody; Histone H2B.k antibody; H2B/k antibody; Histone H2B.l antibody; H2B/l antibody
  • 宿主：
    Rabbit
  • 反應種屬：
    Human
  • 免疫原：
    Peptide sequence around site of Mono-methyl-Lys (12) derived from Human Histone H2B type 1-C/E/F/G/I
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Polyclonal
  • 抗體亞型：
    IgG
  • 純化方式：
    Antigen Affinity Purified
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB, ICC, ChIP
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:100-1:1000
    ICC 1:20-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.; Has broad antibacterial activity. May contribute to the formation of the functional antimicrobial barrier of the colonic epithelium, and to the bactericidal activity of amniotic fluid.
  • 亞細胞定位：
    Nucleus. Chromosome.
  • 蛋白家族：
    Histone H2B family
  • 數據庫鏈接：

    HGNC: 4757

    OMIM: 602798

    KEGG: hsa:3017

    STRING: 9606.ENSP00000366962

    UniGene: Hs.182137



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