在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

MED1 Antibody

  • 中文名稱:
    MED1兔多克隆抗體
  • 貨號:
    CSB-PA004331
  • 規格:
    ¥1090
  • 圖片:
    • Western Blot analysis of K562 cells using TRAP220 Polyclonal Antibody
  • 其他:

產品詳情

  • Uniprot No.:
  • 基因名:
    MED1
  • 別名:
    Activator-recruited cofactor 205 kDa component antibody; ARC205 antibody; CRSP1 antibody; CRSP200 antibody; DRIP205 antibody; DRIP230 antibody; MED1 antibody; MED1_HUMAN antibody; Mediator complex subunit 1 antibody; Mediator of RNA polymerase II transcription subunit 1 antibody; p53 regulatory protein RB18A antibody; PBP antibody; Peroxisome proliferator-activated receptor-binding protein antibody; PPAR binding protein antibody; PPAR-binding protein antibody; PPARBP antibody; PPARGBP antibody; RB18A antibody; Thyroid hormone receptor-associated protein complex 220 kDa component antibody; Thyroid receptor-interacting protein 2 antibody; TR-interacting protein 2 antibody; Trap220 antibody; TRIP-2 antibody; TRIP2 antibody; Vitamin D receptor-interacting protein complex component DRIP205 antibody
  • 宿主:
    Rabbit
  • 反應種屬:
    Human,Mouse
  • 免疫原:
    Synthesized peptide derived from Human TRAP220 around the non-phosphorylation site of T1457.
  • 免疫原種屬:
    Homo sapiens (Human)
  • 標記方式:
    Non-conjugated
  • 抗體亞型:
    IgG
  • 純化方式:
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 濃度:
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液:
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  • 產品提供形式:
    Liquid
  • 應用范圍:
    WB, IF, ELISA
  • 推薦稀釋比:
    Application Recommended Dilution
    WB 1:500-1:2000
    IF 1:200-1:1000
    ELISA 1:10000
  • Protocols:
  • 儲存條件:
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期:
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途:
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

平均分:
5.0分 - 1 個評價

實驗應用:IF

樣品信息:樣本為4T1細胞,小鼠乳腺癌細胞

稀釋比:1:600

產品評價: 我使用CSB-PA004331抗體,進行免疫熒光(IF)實驗,檢測樣本為小鼠乳腺癌細胞4T1。樣品經過給藥(帶綠光)后,用4%多聚甲醛固定后,使用曲拉通100通透,1%BSA封閉后,孵育MED1抗體,后孵育二抗(coralite594)。經共聚焦成像可知,dapi為細胞核,綠光通道為藥物,紅光通道為med1,藥物與med1共定位,符合預期。

By 關老師

靶點詳情

  • 功能:
    Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Acts as a coactivator for GATA1-mediated transcriptional activation during erythroid differentiation of K562 erythroleukemia cells.
  • 基因功能參考文獻:
    1. High MED1 expression is associated with metastasis in breast Cancer. PMID: 29187405
    2. maintenance of the cancer cell state is dependent on recruitment of Mediator and Cohesin through FOXA and master transcription factors PMID: 27739523
    3. Data suggest that mediator complex subunit 1 (Med1/TRAP220) is a target for checkpoint kinase 2 (Chk2)-mediated phosphorylation and may play a role in cellular DNA damage responses by mediating proper induction of gene transcription upon DNA damage. PMID: 28430840
    4. In a cohort of youth at risk for bipolar disorder, pathway analysis showed an enrichment of the glucocorticoid receptor (GR) pathway with the genes MED1, HSPA1L, GTF2A1 and TAF15, which might underlie the previously reported role of stress response in the risk for bipolar disorder in vulnerable populations. PMID: 28291257
    5. modulation of ESR1-MED1 interactions by cAMP signaling plays a critical role in human decidualization. PMID: 26849466
    6. Our data indicate that MED1 serves as a key mediator in ARv567es induced gene expression PMID: 25481872
    7. Results show that miR-1 is downregulated in osteosarcoma cells but both of its targets Med1 and Med31 were overexpressed suggesting that MiR-1 plays an important role on the proliferation of osteosarcoma cells through regulation of Med1 and Med31. PMID: 24969180
    8. MED1 is required for optimal PRDM16-induced Ucp1 expression PMID: 25644605
    9. no association between SCZ and the SNPs of VDR, suggesting that VDR is not a major gene for SCZ in Chinese Han population. However, our data indicate a potential involvement of VDR SNPs in the susceptibility of risperidone-treated patients to MetS PMID: 24418047
    10. hyperactivated ERK and/or AKT signaling pathways promoted MED1 overexpression in prostate cancer cells. PMID: 23538858
    11. These results demonstrate a role for MED1 in mediating resistance to the pure anti-estrogen fulvestrant both in vitro and in vivo. PMID: 23936234
    12. multiple modes of the GATA1-MED1 axis may help to fine-tune GATA1 function during GATA1-mediated homeostasis events. PMID: 24245781
    13. Data concluded that those of VDR ff genotype may be regarded as "low responders" to vitamin D intake in terms of response of circulating 25(OH)D and certain inflammatory biomarkers. PMID: 23160722
    14. MiR-205 is an epigenetically regulated tumor suppressor that targets MED1. PMID: 22869146
    15. MED1 is recruited to the HER2 gene and required for its expression. PMID: 22964581
    16. Med1 (also known as PBP/RB18A/TRAP220/DRIP205) is a component of the human TRAP/Mediator complex that plays an important role in the transcriptional control of various genes PMID: 22342682
    17. functional communications between the MED1 subunit and the MED24-containing submodule that mediate estrogen receptor functions and growth of both normal mammary epithelial cells and breast carcinoma cells. PMID: 22331469
    18. Studies indicate that the activation domain of p53 (p53AD) binds directly to the MED17 subunit of Mediator, whereas the p53 C-terminal domain (p53CTD) binds the MED1 subunit. PMID: 21326907
    19. These results suggest that Mediator structural shifts induced by activator binding help stably orient pol II prior to transcription initiation within the human mediator-RNA polymerase II-TFIIF assembly. PMID: 22343046
    20. Regulation of androgen receptor-dependent transcription by coactivator MED1 is mediated through a newly discovered noncanonical binding motif. PMID: 22102282
    21. MED1 phosphorylation leads to ubiquitin-conjugating enzyme E2C (UBE2C) locus looping, UBE2C gene expression and cell growth in castration-resistant prostate cancer. PMID: 21556051
    22. establish ARGLU1 as a new MED1-interacting protein required for estrogen-dependent gene transcription and breast cancer cell growth. PMID: 21454576
    23. Vitamin D receptor rs2228570 polymorphism is associated with invasive ovarian carcinoma. PMID: 20473893
    24. The core subunit MED1 facilitates VDR activity and regulating keratinocyte proliferation and differentiation PMID: 20520624
    25. analysis of molecular mechanism of binding TRAP220 coactivator to Retinoid X Receptor alpha, activated by 9-cis retinoic acid PMID: 20398753
    26. analysis of mediator in three different structural states: bound to the activator SREBP-1a, VP16, or an activator-free state PMID: 20534441
    27. Results show that the cells of this aggressive form of breast cancer are genetically preprogrammed to depend on NR1D1 and PBP for the energy production necessary for survival. PMID: 20160030
    28. regulates p53-dependent apoptosis PMID: 11840331
    29. Interaction of PIMT with transcriptional coactivators CBP, p300, and PBP differential role in transcriptional regulation PMID: 11912212
    30. spermine significantly enhanced the interaction between HNF4alpha and full-length DRIP205 in an AF-2, NR-box-dependent manner. Spermine enhanced the interaction of DRIP205 with the VDR , but decreased the interaction of both HNF4alpha and VDR with GRIP1 PMID: 12089346
    31. TFIID and human mediator coactivator (TRAP220) complexes assemble cooperatively on promoter DNA PMID: 12130544
    32. there is a coregulatory role for subunits of this protein in androgen receptor-mediated gene expression PMID: 12218053
    33. examination of regulation by cellular signaling pathways PMID: 12356758
    34. extended LXXLL motif sequence determines the nuclear receptor binding specificity PMID: 12556447
    35. DRIP205 has a role as a coactivator of FXR PMID: 15187081
    36. DRIP205 coactivation of estrogen receptor alpha (ERalpha) involves multiple domains of both proteins PMID: 15471764
    37. RB18A plays a central role to control p53wt and p53mut protein content and functions in cells through a loop of regulation, which involves MDM2 PMID: 15848166
    38. MED1 exists predominantly in a TRAP/Mediator subpopulation enriched in RNA polymerase II and is required for ER-mediated transcription. PMID: 15989967
    39. MED14 and MED1 are used by glucocorticoid receptor in a gene-specific manner, providing a mechanism for promoter selectivity by glucocorticoid receptor PMID: 16239257
    40. ERK-mediated phosphorylation is a regulatory mechanism that controls TRAP220/Med1 expression levels and modulates its functional activity. PMID: 16314496
    41. TRAP220/MED1 plays a novel coregulatory role in facilitating the recruitment of TRAP/Mediator to specific target genes involved in growth and cell cycle progression via GABP PMID: 16574658
    42. Med1 depleted cells exhibited an exacerbated response to retinoids, both in terms transcriptional responses and of cellular differentiation. PMID: 16723356
    43. Expression of TRAP220 mRNA and protein was shown to be decreased significantly in the temporal cortex of patients with epilepsy. PMID: 16934225
    44. Both DRIP205 and SRC-3 are required for the keratinocyte differentiation PMID: 17223341
    45. Recruitment of CBP and TRAP220 was diminished by the overexpression of a MED25 NR box deletion mutant, and by treatment with MED25 siRNA PMID: 17641689
    46. phosphorylation of RXRalpha at serine 260 impaired the recruitment of DRIP205 and other coactivators to the VDR.RXRalpha complex PMID: 18003614
    47. ERK-regulated site in Med1 protein is also essential for up-regulating interferon-induced transcription although not critical for binding to C/EBP-beta PMID: 18339625
    48. ERK phosphorylation of MED1 is a regulatory mechanism that promotes MED1 association with Mediator and, as such, may facilitate a novel feed-forward action of nuclear hormones PMID: 18391015
    49. Decreased MED1 transcript levels are observed in matched normal mucosa when compared with colorectal and ovarian cancers. PMID: 19127118
    50. a decrease of RB18A/MED1 expression in human melanoma cells increases their tumorigenic phenotype. PMID: 19243021

    顯示更多

    收起更多

  • 亞細胞定位:
    Nucleus. Note=A subset of the protein may enter the nucleolus subsequent to phosphorylation by MAPK1 or MAPK3.
  • 蛋白家族:
    Mediator complex subunit 1 family
  • 組織特異性:
    Ubiquitously expressed.
  • 數據庫鏈接:

    HGNC: 9234

    OMIM: 604311

    KEGG: hsa:5469

    STRING: 9606.ENSP00000300651

    UniGene: Hs.643754



主站蜘蛛池模板: 在线|国产精品女主播阳台| 久久国产精品一国产精品| 日韩中文高清在线专区| 欧美变态暴力牲交videos| 在教室伦流澡到高潮hgl动漫 | 亚洲情xo亚洲色xo无码| 午夜影视啪啪免费体验区入口 | 日本一卡2卡3卡4卡无卡免费网站| 亚洲中文字幕久久无码| 成人亚洲欧美一区二区三区| 国产三级精品三级在线专1| 日日摸日日碰夜夜爽av| 老熟妇仑乱视频一区二区| 男人扒开女人双腿猛进视频| 国产成人亚洲无吗淙合青草| 日韩中文无码有码免费视频| 欧美另类videossexo高潮| 免费人妻精品一区二区三区 | 国产成人无码精品久久涩吧| 奶头好大狂揉60分钟视频 | 亚洲男人天堂| 国产97在线 | 亚洲| av大片在线无码永久免费| 免费人成网站视频在线观看国内| 亚洲精品亚洲人成在线下载| 无码av高潮抽搐流白浆| 日韩精品无码专区免费视频| 国内精品久久久久久影院8f| 色噜噜狠狠狠狠色综合久| 日本肉体xxxx裸体137大胆| 国产a线视频播放| 伦埋琪琪久久影院三级| 成品人视频ww入口| 欧洲极品无码一区二区三区 | 婷婷网色偷偷亚洲男人甘肃| 波多野结衣在线观看一码| 深夜国产一区二区三区在线看| 免费三级现频在线观看播放| 久久精品无码专区免费| 天天天欲色欲色www免费| 蜜桃av无码免费看永久|