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  4. HIST1H1A (Ab-21) Antibody

HIST1H1A (Ab-21) Antibody

  • 中文名稱：
    HIST1H1A (Ab-21)兔多克隆抗體
  • 貨號：
    CSB-PA010376PA21nacHU
  • 規(guī)格：
    ￥660
  • 圖片：
    • Immunofluorescent analysis of HepG2 cells using CSB-PA010376PA21nacHU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
    • Chromatin Immunoprecipitation Hela (4*106) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 8µg anti-HIST1H1A (CSB-PA010376PA21nacHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品名稱：
    Rabbit anti-Homo sapiens (Human) HIST1H1A Polyclonal antibody
  • Uniprot No.：
  • 基因名：
    HIST1H1A
  • 別名：
    H1-1 antibody; H1F1 antibody; HIST1H1A antibody; Histone H1.1 antibody; Histone H1a antibody
  • 宿主：
    Rabbit
  • 反應(yīng)種屬：
    Human
  • 免疫原：
    Peptide sequence around site of Lys (21) derived from Human Histone H1.1
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated
  • 克隆類型：
    Polyclonal
  • 抗體亞型：
    IgG
  • 純化方式：
    Antigen Affinity Purified
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, IF, ChIP
  • 推薦稀釋比：
    Application Recommended Dilution
    IF 1:50-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

靶點(diǎn)詳情

  • 功能：
    Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation.
  • 基因功能參考文獻(xiàn)：
    1. Data indicate that IgGs possessing an affinity to histone H1 were isolated by affinity chromatography and size exclusion chromatography. PMID: 28361854
    2. Proper binding of histone H1.1 to chromatin is determined by the simultaneous and synergistic binding of its folded wing helix domain -C-terminal domains to the nucleosome. PMID: 26182371
    3. HIST1 cluster PcG methylation has a role in epigenesis in acute myeloid leukemia PMID: 25482132
    4. Results suggest the potential for histone H1 phosphorylation at threonine 146 as a clinical biomarker in breast cancer. PMID: 24601643
    5. fluorescence recovery after photobleaching analyses suggested that TAF-I beta enhances the dissociation of H1.1 from chromatin in the living cell PMID: 21940793
    6. Data show that the multifunctional histone chaperone NPM1 interacts with linker histone H1 through its first acidic stretch (residues 120-132). PMID: 21425800
    7. the C-terminal domain is the primary determinant of histone H1 binding to chromatin in vivo PMID: 14985337

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  • 亞細(xì)胞定位：
    Nucleus. Chromosome. Note=Mainly localizes in euchromatin.
  • 蛋白家族：
    Histone H1/H5 family
  • 數(shù)據(jù)庫鏈接：

    HGNC: 4715

    OMIM: 142709

    KEGG: hsa:3024

    STRING: 9606.ENSP00000244573

    UniGene: Hs.150206



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