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GFRAL Antibody

  • 中文名稱：
    GFRAL兔多克隆抗體
  • 貨號：
    CSB-PA751020LA01HU
  • 規格：
    ￥440
  • 圖片：
    • Western Blot
      Positive WB detected in: Hela whole cell lysate(20μg), MCF-7 whole cell lysate(20μg), A549 whole cell lysate(20μg), 293 whole cell lysate(20μg), PC-3 whole cell lysate(20μg)Mouse Brain tissue lysate(20μg)
      All lanes: GFRAL antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/20000 dilution
      Predicted band size: 45 kDa
      Observed band size: 45 kDa
      Exposure time: 30s
    • Immunofluorescence staining of Hela cell with CSB-PA751020LA01HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

產品詳情

  • 產品描述：
    GFRAL polyclonal antibody CSB-PA751020LA01HU was produced in the rabbits immunized by using the Recombinant Human GDNF family receptor alpha-like protein (19-351AA). The target protein GFRAL is a brainstem-restricted receptor for GDF15 which regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses. Upon interaction with its ligand (GDF15), GFRAL will interact with RET and induces cellular signaling through activation of MAPK- and AKT- signaling pathways.
    This Rabbit anti-Homo sapiens (Human) GFRAL Polyclonal antibody was tested in WB, IHC and ELISA applications. The non-conjugated IgG got purified by protein G and reached up to 95% in purity. It reacts with the FLG proteins of human or mouse-origin and may be used to detect the endogenous levels of FLG protein.
  • 產品名稱：
    Rabbit anti-Homo sapiens (Human) GFRAL Polyclonal antibody
  • Uniprot No.：
  • 基因名：
  • 別名：
    bA360D14.1 antibody; C6orf144 antibody; GDNF family receptor alpha-like antibody; Gfral antibody; GFRAL_HUMAN antibody; GRAL antibody; IVFI9356 antibody; UNQ9356 antibody; UNQ9356/PRO34128 antibody
  • 宿主：
    Rabbit
  • 反應種屬：
    Human,mouse
  • 免疫原：
    Recombinant Human GDNF family receptor alpha-like protein (19-351AA)
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Polyclonal
  • 抗體亞型：
    IgG
  • 純化方式：
    Affinity-purified
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 產品提供形式：
    Liquid
  • 應用范圍：
    WB, IF
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:1000-1:2000
    IF 1:20-1:100
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    Brainstem-restricted receptor for GDF15 which regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses. Upon interaction with its ligand, GDF15, interacts with RET and induces cellular signaling through activation of MAPK- and AKT- signaling pathways.
  • 亞細胞定位：
    Cell membrane; Single-pass membrane protein; Extracellular side.
  • 蛋白家族：
    GDNFR family
  • 組織特異性：
    Expressed in the brainstem, restricted to cells in the area postrema and the immediately adjacent region of the nucleus tractus solitarius (at protein level). Detected at low levels in testis and adipose tissue.
  • 數據庫鏈接：

    HGNC: 32789

    OMIM: 617837

    KEGG: hsa:389400

    STRING: 9606.ENSP00000343636

    UniGene: Hs.526967



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