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GEMIN4 Antibody

  • 中文名稱:
    GEMIN4兔多克隆抗體
  • 貨號:
    CSB-PA009363LA01HU
  • 規格:
    ¥440
  • 圖片:
    • IHC image of CSB-PA009363LA01HU diluted at 1:400 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of A549 cells with CSB-PA009363LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他:

產品詳情

  • 產品名稱:
    Rabbit anti-Homo sapiens (Human) GEMIN4 Polyclonal antibody
  • Uniprot No.:
  • 基因名:
    GEMIN4
  • 別名:
    GEMIN4 antibody; Gem-associated protein 4 antibody; Gemin-4 antibody; Component of gems 4 antibody; p97 antibody
  • 宿主:
    Rabbit
  • 反應種屬:
    Human
  • 免疫原:
    Recombinant Human Gem-associated protein 4 protein (741-848AA)
  • 免疫原種屬:
    Homo sapiens (Human)
  • 標記方式:
    Non-conjugated

    本頁面中的產品,GEMIN4 Antibody (CSB-PA009363LA01HU),的標記方式是Non-conjugated。對于GEMIN4 Antibody,我們還提供其他標記。見下表:

    可提供標記
    標記方式 貨號 產品名稱 應用
    HRP CSB-PA009363LB01HU GEMIN4 Antibody, HRP conjugated ELISA
    FITC CSB-PA009363LC01HU GEMIN4 Antibody, FITC conjugated
    Biotin CSB-PA009363LD01HU GEMIN4 Antibody, Biotin conjugated ELISA
  • 克隆類型:
    Polyclonal
  • 抗體亞型:
    IgG
  • 純化方式:
    >95%, Protein G purified
  • 濃度:
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液:
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 產品提供形式:
    Liquid
  • 應用范圍:
    ELISA, IHC, IF
  • 推薦稀釋比:
    Application Recommended Dilution
    IHC 1:200-1:500
    IF 1:50-1:200
  • Protocols:
  • 儲存條件:
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期:
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途:
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能:
    The SMN complex catalyzes the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome, and thereby plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP (Sm core). In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. To assemble core snRNPs, the SMN complex accepts the trapped 5Sm proteins from CLNS1A forming an intermediate. Binding of snRNA inside 5Sm triggers eviction of the SMN complex, thereby allowing binding of SNRPD3 and SNRPB to complete assembly of the core snRNP.
  • 基因功能參考文獻:
    1. These findings indicate that GEMIN4 functions as a novel coregulator of the MR. PMID: 25555524
    2. the association between the GEMIN4 gene and the risk of prostate cancer PMID: 22506892
    3. zinc-finger proteins were identified as HCAP1-interacting proteins PMID: 12869526
    4. A haplotype of HCAP1 with minor alleles at 5 single nucleotide polymorphisms may be related to cancer susceptibility: its expression causes up-regulation of genes involved in cell growth and down-regulation of genes involved in apoptosis and DNA repair. PMID: 14603441
    5. A canonical nuclear import sequence in the N-terminus of Gemin4 was identified. PMID: 18675250

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  • 亞細胞定位:
    Cytoplasm. Nucleus. Nucleus, nucleolus. Nucleus, gem. Note=Localized in subnuclear structures next to coiled bodies, called gems, which are highly enriched in spliceosomal snRNPs and in the nucleolus.
  • 數據庫鏈接:

    HGNC: 15717

    OMIM: 606969

    KEGG: hsa:50628

    STRING: 9606.ENSP00000321706

    UniGene: Hs.499620



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