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  4. FEV Antibody

FEV Antibody

  • 中文名稱(chēng)：
    FEV兔多克隆抗體
  • 貨號(hào)：
    CSB-PA858717LA01HU
  • 規(guī)格：
    ￥440
  • 圖片：
    • Western Blot
      Positive WB detected in: HL60 whole cell lysate, Rat brain tissue
      All lanes: FEV antibody at 5.9µg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 26 kDa
      Observed band size: 26 kDa
    • IHC image of CSB-PA858717LA01HU diluted at 1:300 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • IHC image of CSB-PA858717LA01HU diluted at 1:300 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of Hela cells with CSB-PA858717LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品名稱(chēng)：
    Rabbit anti-Homo sapiens (Human) FEV Polyclonal antibody
  • Uniprot No.：
  • 基因名：
    FEV
  • 別名：
    ETS-domain transcription factor antibody; FEV (ETS oncogene family) antibody; FEV antibody; FEV ETS transcription factor antibody; FEV_HUMAN antibody; Fifth Ewing sarcoma variant antibody; Fifth Ewing variant protein antibody; HSRNAFEV antibody; mPet1 antibody; PC12 ETS domain-containing transcription factor 1 antibody; PC12 ETS factor 1 antibody; Pet-1 antibody; Protein FEV antibody
  • 宿主：
    Rabbit
  • 反應(yīng)種屬：
    Human, Rat
  • 免疫原：
    Recombinant Human Protein FEV protein (1-97AA)
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated

    本頁(yè)面中的產(chǎn)品，F(xiàn)EV Antibody (CSB-PA858717LA01HU)，的標(biāo)記方式是Non-conjugated。對(duì)于FEV Antibody，我們還提供其他標(biāo)記。見(jiàn)下表：

    可提供標(biāo)記
    標(biāo)記方式 貨號(hào) 產(chǎn)品名稱(chēng) 應(yīng)用
    HRP CSB-PA858717LB01HU FEV Antibody, HRP conjugated ELISA
    FITC CSB-PA858717LC01HU FEV Antibody, FITC conjugated
    Biotin CSB-PA858717LD01HU FEV Antibody, Biotin conjugated ELISA
  • 克隆類(lèi)型：
    Polyclonal
  • 抗體亞型：
    IgG
  • 純化方式：
    >95%, Protein G purified
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, WB, IHC, IF
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:200-1:500
    IF 1:50-1:200
  • Protocols：
  • 儲(chǔ)存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Functions as a transcriptional regulator. According to PubMed:12761502, it functions as a transcriptional repressor. Functions in the differentiation and the maintenance of the central serotonergic neurons. May play a role in cell growth.
  • 基因功能參考文獻(xiàn)：
    1. This study adds to the growing evidence on the association of single- and multiple-risk variants in DRD3, DRD4, and FEV with aggressive behavior in Chinese adolescents. PMID: 28846959
    2. identified FEV is unique to fetal HSCs and stably expressed in leukemic cells of prenatal origin PMID: 27807368
    3. Genetic variation in FEV1 was associated with serum 25-Hydroxyvitamin D status. PMID: 26122139
    4. novel evidence for the role of Pet-1 in human amygdala threat processing extends literature demonstrating the influence of genetic variation in the serotonin system on emotional regulation PMID: 24100022
    5. Fev-ERK signaling is essential for hemogenic endothelium-based hematopoietic stem cell development. PMID: 23591790
    6. our data argue against an exclusive role of FEV in the adult human brain serotonergic system and genetic analyses did not suggest that FEV variation adds to the genetic liability towards affective disorders PMID: 20480378
    7. FEV identifies serotonin-producing cells in normal and neoplastic small intestine. PMID: 20048018
    8. Serotonin-related FEV gene variant in the sudden infant death syndrome is a common polymorphism in the African-American population. PMID: 19707175
    9. FEV acts as a transcriptional repressor through its DNA-binding ETS domain and alanine-rich domain. PMID: 12761502
    10. We showed that fev is exclusively expressed in the midline part of the human brainstem containing raphe nuclei, which also specifically expressed 5-HT transporter (sert) and tryptophan hydroxylase (tph), two markers of the 5-HT neurotransmitter system. PMID: 15003288
    11. Analysis of transgene expression in Pet-1 null mice indicates that Pet-1 is required to maintain the activity of the Pet-1 enhancer region in a subset of serotonin (5-HT) neurons. PMID: 15758173
    12. Variation in FEV gene observed exclusively in a subset of African American sudden infant death syndrome cases may help explain the observed abnormalities of this system in some SIDS cases and the ethnic disparity. PMID: 17597646

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  • 相關(guān)疾病：
    Sudden infant death syndrome (SIDS)
  • 亞細(xì)胞定位：
    Nucleus.
  • 蛋白家族：
    ETS family
  • 組織特異性：
    In brain, exclusively expressed in the major serotonergic neurons of the dorsal and median raphe nuclei located in the midbrain and pons. Also detected in prostate and small intestine.
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 18562

    OMIM: 272120

    KEGG: hsa:54738

    STRING: 9606.ENSP00000295727

    UniGene: Hs.234759



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