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FET3 Antibody

  • 中文名稱:
    FET3 Antibody
  • 貨號:
    CSB-PA340040XA01SVG
  • 規格:
    ¥880
  • 圖片:
    • Western Blot
      Positive WB detected in: recombinant protein
      All lanes:FET3 Antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/20000 dilution
      Predicted band size: 27.6 kDa
      Observed band size: 35、34 kDa
  • 其他:

產品詳情

  • 產品名稱:
    Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FET3 Polyclonal antibody
  • Uniprot No.:
  • 基因名:
    FET3
  • 別名:
    Iron transport multicopper oxidase FET3 (EC 1.-.-.-) FET3 YMR058W YM9796.11
  • 宿主:
    Rabbit
  • 反應種屬:
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 免疫原:
    Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FET3 protein (377-559aa)
  • 免疫原種屬:
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 標記方式:
    Non-conjugated
  • 克隆類型:
    Polyclonal
  • 抗體亞型:
    IgG
  • 純化方式:
    Antigen Affinity Purified
  • 濃度:
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液:
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 產品提供形式:
    Liquid
  • 應用范圍:
    ELISA, WB
  • 推薦稀釋比:
    Application Recommended Dilution
    WB 1:500-1:5000
  • Protocols:
  • 儲存條件:
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期:
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途:
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能:
    Iron transport multicopper ferroxidase required for Fe(2+) ion high affinity uptake. Required to oxidize Fe(2+) and release it from the transporter. Essential component of copper-dependent iron transport.
  • 基因功能參考文獻:
    1. Presence of crowding agents does not affect Fet3p structural content but increases thermal resistance. The observations are compatible with ordering of a non-optimal substrate-binding site and restricted internal dynamics. PMID: 19932772
    2. The D94 residue appears to activate a proton of the type 2 Cu(+)-bound water for participation in the transition state. PMID: 15835897
    3. Substitution of Asp320 with asparagine essentially abolished the Fet3-dependent iron transport activity. All other mutants (D278Q, D279N, D312N and D315I) behaved essentially as the wild-type protein. PMID: 16032772
    4. results are consistent with a (minimal) heterodimer model of the Fet3p.Ftr1p complex that supports the trafficking of iron from Fet3p to Ftr1p for iron permeation across the yeast plasma membrane PMID: 16522632
    5. D283, E185, and D409 in Fet3p provide a Fe(II) binding site that actually favors ferric iron. PMID: 17042492
    6. Structure-function analysis of the cuprous oxidase activity in Fet3p from Saccharomyces cerevisiae PMID: 17220296
    7. We find that unfolding reactions of apo and different holo forms of Fet3p are irreversible reactions that depend on the scan rate and there is a metal-induced tradeoff between overall protein stability and metal coordination PMID: 19033465

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  • 亞細胞定位:
    Cell membrane; Single-pass type I membrane protein; Extracellular side.
  • 蛋白家族:
    Multicopper oxidase family
  • 數據庫鏈接:

    KEGG: sce:YMR058W

    STRING: 4932.YMR058W



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