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  4. FCHO1 Antibody

FCHO1 Antibody

  • 中文名稱：
    FCHO1兔多克隆抗體
  • 貨號：
    CSB-PA008546LA01HU
  • 規格：
    ￥440
  • 圖片：
    • IHC image of CSB-PA008546LA01HU diluted at 1:300 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • IHC image of CSB-PA008546LA01HU diluted at 1:300 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of MCF-7 cells with CSB-PA008546LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

產品詳情

  • 產品名稱：
    Rabbit anti-Homo sapiens (Human) FCHO1 Polyclonal antibody
  • Uniprot No.：
  • 基因名：
    FCHO1
  • 別名：
    FCHO1 antibody; KIAA0290F-BAR domain only protein 1 antibody
  • 宿主：
    Rabbit
  • 反應種屬：
    Human
  • 免疫原：
    Recombinant Human F-BAR domain only protein 1 protein (45-180AA)
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated

    本頁面中的產品，FCHO1 Antibody (CSB-PA008546LA01HU)，的標記方式是Non-conjugated。對于FCHO1 Antibody，我們還提供其他標記。見下表：

    可提供標記
    標記方式 貨號 產品名稱 應用
    HRP CSB-PA008546LB01HU FCHO1 Antibody, HRP conjugated ELISA
    FITC CSB-PA008546LC01HU FCHO1 Antibody, FITC conjugated
    Biotin CSB-PA008546LD01HU FCHO1 Antibody, Biotin conjugated ELISA
  • 克隆類型：
    Polyclonal
  • 抗體亞型：
    IgG
  • 純化方式：
    >95%, Protein G purified
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, IHC, IF
  • 推薦稀釋比：
    Application Recommended Dilution
    IHC 1:200-1:500
    IF 1:50-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    Functions in an early step of clathrin-mediated endocytosis. Has both a membrane binding/bending activity and the ability to recruit proteins essential to the formation of functional clathrin-coated pits. May regulate Bmp signaling by regulating clathrin-mediated endocytosis of Bmp receptors.
  • 基因功能參考文獻：
    1. Fcho1/2Eps15/RAP-2 ternary complexes to facilitate conformational activation of AP-2 by the Fcho1/2 interdomain linker to promote AP-2 cargo engagement. PMID: 27237791
    2. The central linker of FCHO proteins acts as an allosteric regulator of the prime endocytic adaptor, AP-2. PMID: 25303365
    3. show that the mu-homology domain of FCHO1/2 represents an endocytic interaction hub PMID: 22484487
    4. study reports FCHo1/2 were required for plasma membrane clathrin-coated vesicle (CCV) budding & marked sites of CCV formation PMID: 20448150
    5. Involved in clathrin-coated vesicle formation. PMID: 17617719

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  • 亞細胞定位：
    Membrane, clathrin-coated pit; Peripheral membrane protein; Cytoplasmic side. Note=Associated with forming but not mature clathrin-coated vesicles. The recruitment to coated-pits precede the one of clathrin and the adaptor protein complex AP-2. According to PubMed:17617719 it may also dynamically associate with Golgi/TGN membranes.
  • 蛋白家族：
    FCHO family
  • 數據庫鏈接：

    HGNC: 29002

    OMIM: 613437

    KEGG: hsa:23149

    STRING: 9606.ENSP00000252771

    UniGene: Hs.96485



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