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  4. EIF4A2 Antibody

EIF4A2 Antibody

  • 中文名稱：
    EIF4A2兔多克隆抗體
  • 貨號：
    CSB-PA00384A0Rb
  • 規格：
    ￥440
  • 圖片：
    • Western Blot
      Positive WB detected in: HepG2 whole cell lysate, Jurkat whole cell lysate, Raji whole cell lysate, 293 whole cell lysate
      All lanes: EIF4A2 antibody at 3µg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 47 kDa
      Observed band size: 47 kDa
    • Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA00384A0Rb at dilution of 1:100
    • Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA00384A0Rb at dilution of 1:100
    • Immunofluorescence staining of A549 cells with CSB-PA00384A0Rb at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

產品詳情

  • 產品名稱：
    Rabbit anti-Homo sapiens (Human) EIF4A2 Polyclonal antibody
  • Uniprot No.：
  • 基因名：
    EIF4A2
  • 別名：
    ATP dependent RNA helicase eIF4A 2 antibody; ATP-dependent RNA helicase eIF4A-2 antibody; BM-010 antibody; DDX2B antibody; eIF 4A II antibody; eIF-4A-II antibody; EIF4A antibody; eIF4A II antibody; eIF4A-II antibody; EIF4A2 antibody; EIF4F antibody; Eukaryotic initiation factor 4A II antibody; Eukaryotic initiation factor 4A-II antibody; eukaryotic translation initiation factor 4A isoform 2 antibody; Eukaryotic translation initiation factor 4A2 antibody; IF4A2_HUMAN antibody; N-terminally processed antibody
  • 宿主：
    Rabbit
  • 反應種屬：
    Human
  • 免疫原：
    Recombinant Human Eukaryotic initiation factor 4A-II protein (1-407AA)
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated

    本頁面中的產品，EIF4A2 Antibody (CSB-PA00384A0Rb)，的標記方式是Non-conjugated。對于EIF4A2 Antibody，我們還提供其他標記。見下表：

    可提供標記
    標記方式 貨號 產品名稱 應用
    HRP CSB-PA00384B0Rb EIF4A2 Antibody, HRP conjugated ELISA
    FITC CSB-PA00384C0Rb EIF4A2 Antibody, FITC conjugated
    Biotin CSB-PA00384D0Rb EIF4A2 Antibody, Biotin conjugated ELISA
  • 克隆類型：
    Polyclonal
  • 抗體亞型：
    IgG
  • 純化方式：
    >95%, Protein G purified
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB, IHC, IF
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:1000-1:5000
    IHC 1:20-1:200
    IF 1:50-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.
  • 基因功能參考文獻：
    1. eIF4A2 is recruited to stress granules, suggesting sumoylation of eIF4A2 correlates with its recruitment to stress granules. PMID: 27160682
    2. expression is an independent prognostic factor for patients with non-small-cell lung cancer for both overall survival and disease-free survival PMID: 23867391
    3. These data support a linear model for miRNA-mediated gene regulation in which translational repression via eIF4A2 is required first, followed by mRNA destabilization. PMID: 23559250
    4. interacted with NS5B protein, and the two proteins were shown to be partially colocalized in the perinuclear region PMID: 11922617
    5. Single nucleotide polymorphisms in two relevant candidate genes for glucose homeostasis, kininogen (KNG1), and eukaryotic translation initiation factor 4alpha2 (EIF4A2) linked to type 2 diabetes. PMID: 16567544
    6. subcellular distribution of eIF4F components may potentiate the complex assembly PMID: 18250159
    7. A feedforward loop involving c-Myc and eIF4F that serves to link transcription and translation and that could contribute to the effects of c-Myc on cell proliferation and neoplastic growth. PMID: 18593934
    8. This protein has been found differentially expressed in the temporal lobe from patients with schizophrenia. PMID: 19034380

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  • 蛋白家族：
    DEAD box helicase family, eIF4A subfamily
  • 數據庫鏈接：

    HGNC: 3284

    OMIM: 601102

    KEGG: hsa:1974

    STRING: 9606.ENSP00000326381

    UniGene: Hs.518475



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