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Your Good Partner in Biology Research

  • 中文名稱:
    EIF3K兔多克隆抗體
  • 貨號(hào):
    CSB-PA866204ESR1HU
  • 規(guī)格:
    ¥440
  • 圖片:
    • Western blot
      All lanes: EIF3K antibody at 1.72µg/ml
      Lane 1: THP-1 whole cell lysate
      Lane 2: HT29 whole cell lysate
      Lane 3: 293T whole cell lysate
      Lane 4: Hela whole cell lysate
      Secondary
      Goat polyclonal to rabbit IgG at 1/10000 dilution
      Predicted band size: 26, 25 kDa
      Observed band size: 26 kDa
    • Immunohistochemistry of paraffin-embedded human liver cancer using CSB-PA866204ESR1HU at dilution of 1:100
    • Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA866204ESR1HU at dilution of 1:100
    • Immunoprecipitating EIF3K in Hela whole cell lysate
      Lane 1: Rabbit control IgG instead of (1µg) instead of CSB-PA866204ESR1HU in Hela whole cell lysate. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000)
      Lane 2: CSB-PA866204ESR1HU (4µg) + Hela whole cell lysate (500µg)
      Lane 3: Hela whole cell lysate (20µg)
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品名稱:
    Rabbit anti-Homo sapiens (Human) EIF3K Polyclonal antibody
  • Uniprot No.:
  • 基因名:
    EIF3K
  • 別名:
    ARG134 antibody; eIF 3 p25 antibody; eIF 3 p28 antibody; eIF-3 p25 antibody; eIF-3 p28 antibody; EIF3-p28 antibody; eif3k antibody; EIF3K_HUMAN antibody; EIF3S12 antibody; Eukaryotic translation initiation factor 3 subunit 12 antibody; Eukaryotic translation initiation factor 3 subunit K antibody; HSPC029 antibody; M9 antibody; MSTP001 antibody; Muscle specific gene M9 protein antibody; Muscle-specific gene M9 protein antibody; PLAC 24 antibody; PLAC-24 antibody; PLAC24 antibody; PRO1474 antibody; PTD001 antibody
  • 宿主:
    Rabbit
  • 反應(yīng)種屬:
    Human
  • 免疫原:
    Recombinant Human Eukaryotic translation initiation factor 3 subunit K protein (1-218AA)
  • 免疫原種屬:
    Homo sapiens (Human)
  • 標(biāo)記方式:
    Non-conjugated
  • 克隆類型:
    Polyclonal
  • 抗體亞型:
    IgG
  • 純化方式:
    Antigen Affinity Purified
  • 濃度:
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液:
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • 產(chǎn)品提供形式:
    Liquid
  • 應(yīng)用范圍:
    ELISA, WB, IHC, IP
  • 推薦稀釋比:
    Application Recommended Dilution
    WB 1:1000-1:5000
    IHC 1:20-1:200
    IP 1:200-1:2000
  • Protocols:
  • 儲(chǔ)存條件:
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期:
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途:
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能:
    Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression.
  • 基因功能參考文獻(xiàn):
    1. eIF3K subunit interacts directly with the PML protein. PMID: 24036361
    2. identification of a novel 24-kDa protein that binds directly to dynein intermediate chain [PLAC-24] PMID: 12006665
    3. eIF3k has three putative protein-binding surfaces and has potential RNA binding activity PMID: 15180986
    4. cyclin D3 specifically interacted with eIF3k through its C-terminal domain; eIF3k distributed both in nucleus and cytoplasm and colocalized with cyclin D3 PMID: 15327989
    5. eIF3k, originally identified as the smallest subunit of eukaryotic translation initiation factor 3 (eIF3) complexes, also localizes to keratin intermediate filaments and physically associates with K18 in epithelial cells. PMID: 18577580

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  • 亞細(xì)胞定位:
    Nucleus. Cytoplasm.
  • 蛋白家族:
    EIF-3 subunit K family
  • 組織特異性:
    Ubiquitous, with the highest levels of expression in brain, testis and kidney.
  • 數(shù)據(jù)庫鏈接:

    HGNC: 24656

    OMIM: 609596

    KEGG: hsa:27335

    STRING: 9606.ENSP00000248342

    UniGene: Hs.314359



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