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  4. DOHH Antibody

DOHH Antibody

  • 中文名稱：
    DOHH兔多克隆抗體
  • 貨號(hào)：
    CSB-PA880104LA01HU
  • 規(guī)格：
    ￥440
  • 圖片：
    • Immunofluorescence staining of PC-3 cells with CSB-PA880104LA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品名稱：
    Rabbit anti-Homo sapiens (Human) DOHH Polyclonal antibody
  • Uniprot No.：
  • 基因名：
    DOHH
  • 別名：
    Deoxyhypusine dioxygenase antibody; Deoxyhypusine hydroxylase antibody; Deoxyhypusine hydroxylase/monooxygenase antibody; Deoxyhypusine monooxygenase antibody; Dohh antibody; DOHH_HUMAN antibody; hDOHH antibody; HEAT like (PBS lyase) repeat containing 1 antibody; HEAT like PBS lyase repeat containing antibody; HEAT like repeat containing protein 1 antibody; HEAT-like repeat-containing protein 1 antibody; HLRC1 antibody; MGC4293 antibody
  • 宿主：
    Rabbit
  • 反應(yīng)種屬：
    Human
  • 免疫原：
    Recombinant Human Deoxyhypusine hydroxylase protein (79-159AA)
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated

    本頁(yè)面中的產(chǎn)品，DOHH Antibody (CSB-PA880104LA01HU)，的標(biāo)記方式是Non-conjugated。對(duì)于DOHH Antibody，我們還提供其他標(biāo)記。見下表：

    可提供標(biāo)記
    標(biāo)記方式 貨號(hào) 產(chǎn)品名稱 應(yīng)用
    HRP CSB-PA880104LB01HU DOHH Antibody, HRP conjugated ELISA
    FITC CSB-PA880104LC01HU DOHH Antibody, FITC conjugated
    Biotin CSB-PA880104LD01HU DOHH Antibody, Biotin conjugated ELISA
  • 克隆類型：
    Polyclonal
  • 抗體亞型：
    IgG
  • 純化方式：
    >95%, Protein G purified
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, IF
  • 推薦稀釋比：
    Application Recommended Dilution
    IF 1:50-1:200
  • Protocols：
  • 儲(chǔ)存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Catalyzes the hydroxylation of the N(6)-(4-aminobutyl)-L-lysine intermediate produced by deoxyhypusine synthase/DHPS on a critical lysine of the eukaryotic translation initiation factor 5A/eIF-5A. This is the second step of the post-translational modification of that lysine into an unusual amino acid residue named hypusine. Hypusination is unique to mature eIF-5A factor and is essential for its function.
  • 基因功能參考文獻(xiàn)：
    1. Data describes 3-D structure of hDOHH in two states, hDOHHperoxo (POX) and a complex of the diiron core with glycerol (GLC) providing an explanation for the extreme longevity of POX and illustrate its substrate specificity for deoxyhypusine-eIF-5A. PMID: 25865244
    2. Here, we show that PCBP1 and PCBP2 also deliver iron to deoxyhypusine hydroxylase (DOHH), the dinuclear iron enzyme required for hypusine modification of the translation factor eukaryotic initiation factor 5A. PMID: 24843120
    3. a novel role for miR-331-3p and miR-642-5p in the control of prostate cancer cell growth via the regulation of DOHH expression and eIF5A activity. PMID: 22908221
    4. The structure and function of DOHH are reported. PMID: 16533814
    5. analysis of the deoxyhypusine hydroxylase-eukaryotic translation initiation factor (eIF5A) interaction PMID: 17213197
    6. Resonance Raman experiments show that its blue chromophore arises from a (mu-1,2-peroxo)diiron(III) center that forms in the reaction of the reduced enzyme with O2, so the peroxo form of hDOHH is unusually stable. PMID: 19706422

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  • 蛋白家族：
    Deoxyhypusine hydroxylase family
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 28662

    OMIM: 611262

    KEGG: hsa:83475

    STRING: 9606.ENSP00000250937

    UniGene: Hs.515064



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