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  4. DME Antibody, HRP conjugated

DME Antibody, HRP conjugated

Rare Species
  • 中文名稱：
    DME兔多克隆抗體, HRP偶聯
  • 貨號：
    CSB-PA839701LB01DOA
  • 規格：
    ￥880
  • 其他：

產品詳情

  • 產品名稱：
    Rabbit anti-Arabidopsis thaliana DME Polyclonal antibody
  • Uniprot No.：
  • 基因名：
    DME
  • 別名：
    DME antibody; At5g04560/At5g04570/At5g04580 antibody; T32M21.160/T32M21.170/T32M21.180Transcriptional activator DEMETER antibody; EC 3.2.2.- antibody; DNA glycosylase-related protein DME antibody
  • 宿主：
    Rabbit
  • 反應種屬：
    Arabidopsis thaliana
  • 免疫原：
    Recombinant Arabidopsis thaliana Transcriptional activator DEMETER protein (1-320AA)
  • 免疫原種屬：
    Arabidopsis thaliana
  • 標記方式：
    HRP
  • 克隆類型：
    Polyclonal
  • 抗體亞型：
    IgG
  • 純化方式：
    >95%, Protein G purified
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    Transcriptional activator involved in gene imprinting. Catalyzes the release of 5-methylcytosine (5-meC) from DNA by a glycosylase/lyase mechanism. Allows the expression of the maternal copy of the imprinted MEA gene before fertilization, possibly by antagonizing or suppressing DNA methylation on target promoter. Probably acts by nicking the MEA promoter. Required for stable reproducible patterns of floral and vegetative development.
  • 基因功能參考文獻：
    1. DME and ROS1 have a significant 5-hydroxymethylcytosine (5hmC) excision activity in vitro, although the study detected no 5hmC in Arabidopsis, suggesting that it is very unlikely for plants to utilize 5hmC as a DNA demethylation intermediate. PMID: 24661881
    2. Histone H1 is involved in DME-mediated DNA methylation and gene regulation at imprinted loci. PMID: 22519754
    3. DME is active and demethylates similar genes and transposons in the genomes of the vegetative and central cells in the male and female gametophytes PMID: 21518889
    4. Analyses indicate that AtLIG1 acts downstream of DME. The removal of methylated cytosine residues by DME involves the creation of DNA single-strand breaks and our results suggest that AtLIG1 repairs these breaks. PMID: 20023162
    5. Demethylation by DEMETER is necessary for genomic imprinting and defines the genomic methylation profile of Arabidopsis genes. PMID: 17409185
    6. Gene ontology analysis identified three molecular functions enriched in the DME-inducible RNA list: DNA or RNA binding, kinase activity, and transcription factor activity. PMID: 17964287
    7. control DNA methylation in Arabidopsis thaliana.[review] PMID: 18536342

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  • 亞細胞定位：
    Nucleus.
  • 蛋白家族：
    DNA glycosylase family, DEMETER subfamily
  • 組織特異性：
    Mainly expressed in immature flower buds, then decreases as the flower matures. Expressed in the ovule carpels, but not expressed in pollen stamens. Expressed in developing and mature ovules (stages 12-14), then strongly decreases after fertilization.
  • 數據庫鏈接：

    KEGG: ath:AT5G04560

    STRING: 3702.AT5G04560.2

    UniGene: At.33104



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