在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

CSTB Antibody

  • 中文名稱:
    CSTB兔多克隆抗體
  • 貨號:
    CSB-PA00224A0Rb
  • 規格:
    ¥440
  • 圖片:
    • Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA00224A0Rb at dilution of 1:100
    • IHC image of CSB-PA00224A0Rb diluted at 1:400 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Western Blot
      Positive WB detected in: HepG2 whole cell lysate, MCF-7 whole cell lysate, A549 whole cell lysate, U87 whole cell lysate, 293T whole cell lysate
      All lanes: CSTB antibody at 5.36µg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 12 kDa
      Observed band size: 12 kDa
    • Immunofluorescence staining of HepG2 cells with CSB-PA00224A0Rb at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Immunoprecipitating CSTB in HepG2 whole cell lysate
      Lane 1: Rabbit control IgG instead of CSB-PA00224A0Rb in HepG2 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
      Lane 2: CSB-PA00224A0Rb (6µg) + HepG2 whole cell lysate (500µg)
      Lane 3: HepG2 whole cell lysate (20µg)
  • 其他:

產品詳情

  • 產品描述:

    The CSTB polyclonal antibody is produced in rabbits using recombinant human CSTB (1-98aa) as an immunogen. This antibody can be used to detect CSTB protein in human samples. The antibody has been purified using Protein G, reaching up to 95% in purity.

    The CSTB antibody can be used to study the function and regulation of CSTB protein in a variety of applications, including ELISA, WB, IHC, IF, and IP. In ELISA, this CSTB antibody can be used to quantitatively detect CSTB protein in a sample. In WB, it can be used to detect the presence of CSTB protein in a sample and determine its size. In IHC, this CSTB antibody can be used to detect the localization and expression levels of CSTB protein in tissues. In IF, this CSTB antibody can be used to detect the presence and localization of CSTB protein in cells. In IP, this antibody can be used to isolate and purify CSTB protein from a complex mixture of proteins.

  • 產品名稱:
    Rabbit anti-Homo sapiens (Human) CSTB Polyclonal antibody
  • Uniprot No.:
  • 基因名:
    CSTB
  • 別名:
    CHROW21 antibody; CPI B antibody; CPI-B antibody; CST 6 antibody; CST6 antibody; CSTB antibody; Cystatin B (stefin B) antibody; Cystatin B antibody; Cystatin-B antibody; CYTB antibody; CYTB_HUMAN antibody; EPM1 antibody; EPM1A antibody; Liver thiol proteinase inhibitor antibody; PME antibody; Stefin-B antibody; STF B antibody; STFB antibody; ULD antibody
  • 宿主:
    Rabbit
  • 反應種屬:
    Human
  • 免疫原:
    Recombinant Human Cystatin-B protein (1-98AA)
  • 免疫原種屬:
    Homo sapiens (Human)
  • 標記方式:
    Non-conjugated

    本頁面中的產品,CSTB Antibody (CSB-PA00224A0Rb),的標記方式是Non-conjugated。對于CSTB Antibody,我們還提供其他標記。見下表:

    可提供標記
    標記方式 貨號 產品名稱 應用
    HRP CSB-PA00224B0Rb CSTB Antibody, HRP conjugated ELISA
    FITC CSB-PA00224C0Rb CSTB Antibody, FITC conjugated
    Biotin CSB-PA00224D0Rb CSTB Antibody, Biotin conjugated ELISA
  • 克隆類型:
    Polyclonal
  • 抗體亞型:
    IgG
  • 純化方式:
    >95%, Protein G purified
  • 濃度:
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液:
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 產品提供形式:
    Liquid
  • 應用范圍:
    ELISA, WB, IHC, IF, IP
  • 推薦稀釋比:
    Application Recommended Dilution
    WB 1:1000-1:5000
    IHC 1:100-1:500
    IF 1:50-1:200
    IP 1:200-1:2000
  • Protocols:
  • 儲存條件:
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期:
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途:
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能:
    This is an intracellular thiol proteinase inhibitor. Tightly binding reversible inhibitor of cathepsins L, H and B.
  • 基因功能參考文獻:
    1. cystatin B expression was significantly and inversely correlated with lung tumor stage and tumor grade PMID: 29037838
    2. The results demonstrate that cystatin B interferes with the STAT-1 signaling and IFN-beta-antiviral responses perpetuating HIV in macrophage reservoirs. PMID: 27137788
    3. apoptosis is accompanied by degradation of the cysteine cathepsin inhibitor stefin B (StfB). CatD did not exhibit a crucial role in this step. However, this degradation was partially prevented through pre-incubation with the antioxidant N-acetyl cysteine PMID: 28543404
    4. Homozygous for a c.218dupT (p.His75Serfs*2) mutation in exon 3 of CSTB causes neurodegeneration, progressive cerebral volume loss and diffuse hypomyelination. PMID: 28378817
    5. CSTB downregulation may promote the development of gastric cancer. PMID: 28281969
    6. It was shown that decreased expression of cystatin B enhances cathepsin activity in Niemann-Pick C cerebellar degeneration patient fibroblasts. PMID: 26908626
    7. High expression of stefin B may be an important factor contributing to the development and metastasis of Hepatocellular Carcinoma. PMID: 26753874
    8. CSTB null mutation associated with microcephaly, early developmental delay, and severe dyskinesia. PMID: 26843564
    9. Data shows that CYTB and ANXA4 overexpression may be involved in carcinogenesis and histopathological differentiation of ovarian clear cell carcinoma and suggest they may serve as a potential diagnostic biomarkers. PMID: 25633807
    10. A role for disease-causing mutations in cystatin B gene in patients with juvenile myoclonic epilepsy was not supported. PMID: 25752200
    11. Even though the majority of EPM1 patients have a uniform genetic mutation, the actual size of the longer CSTB expansion mutation allele is likely to have a modulating effect on the age at disease onset, myoclonus severity, and cortical neurophysiology. PMID: 25770194
    12. The study shows detection of stefin B dimers in HEK293 cells and the importance of their residual activity. PMID: 25047918
    13. glutamate dehydrogenase is a euchromatin-associated enzyme, and its H3 clipping activity is regulated by chromatin structure, histone modifications and an in vivo inhibitor. PMID: 25263734
    14. detected a homozygous expansion of dodecamer repeats in the CSTB gene in four patients with clinical diagnosis of ULD. PMID: 23883076
    15. The increased CSTB expression in ovarian tissue represents tumor progression and is dysregulated by the TGF-beta signaling pathway. PMID: 24452274
    16. A reciprocal influence of CSTB and SOD1 at the gene expression level and for a direct interaction of the two proteins, is reported. PMID: 24234043
    17. The present study was performed on two more missense mutants of human stefin B, G50E and Q71P, and they similarly showed numerous aggregates upon overexpression. PMID: 24909779
    18. The co-localization of stefin B wild type and EPM1 mutants with cathepsins showed that cathepsins accumulate around the aggregates formed by the EPM1 mutants. PMID: 23362198
    19. Skull thickening and an increased prevalence of abnormal findings in skeletal radiographs of patients with EPM1 suggest that this condition is connected to defective cystatin B function. PMID: 23010349
    20. This study suggested that CSTB mutations other than the common dodecamer expansion predict particular phenotypes, including marked seizure severity and polymorphous seizure types. PMID: 23205931
    21. Elevated StefA mRNA level is associated with invasive glioblastoma. PMID: 22287159
    22. S-glutathionylation and S-cysteinylation were described as extensive PTM of a salivary protein and the first time that these PTMs were detected in naturally occurring cystatin B. PMID: 22057043
    23. patients compound heterozygous for the dodecamer repeat expansion and the c.202C>T mutations seem to have a severer form of Unverricht-Lundborg disease (EPM1) than patients homozygous for the expansion mutation PMID: 21757863
    24. At pH 7.0 the mutant H75W folded in three kinetic phases to a native-like intermediate, analogous to folding of stefin B at pH 4.8. PMID: 22033403
    25. Intracellular stefin b aggregation shows a negative correlation with cell survival PMID: 20078424
    26. Stefin B interacts with histones and cathepsin L in the nucleus PMID: 20075068
    27. oligomers of stefin B and amyloid-beta interact in vitro and in cells PMID: 19955183
    28. Oligonucleotides containing EPM1 repeat adopt secondary structures that may facilitate strand slippage thereby causing the expansion. PMID: 11697734
    29. Intramolecular i-motif structure at acidic pH for progressive myoclonus epilepsy (EPM1) repeat d(CCCCGCCCCGCG)n. PMID: 11697735
    30. analysed eight markers flanking CSTB(GT10-D21S1890-D21S1885-D21S2040-D21S1259- CSTB-D21S1912-PFKL-D21S171) and one intragenic variant in the CSTB 3' UTR (A2575G) PMID: 12215838
    31. first demonstration of cysteine protease activity being regulated by CSTB activity in a biological context; effects of decreased CSTB activity in EPM1 pathogenesis may be mediated by cathepsins through increased activity of cathepsins S and L PMID: 12452481
    32. Prefibrillar oligomers/aggregates of stefin B also increase the surface pressure at an air-water interface, i.e. they have amphipathic character and are surface seeking. PMID: 15955063
    33. These data show that cystatin B inhibits bone resorption by down-regulating intracellular cathepsin K activity despite increased osteoclast survival. PMID: 16321512
    34. Study shows that copper binding by stefin B inhibits the amyloid fibril formation and, to a lesser degree, the initial aggregation. PMID: 16939620
    35. Several alternatively spliced CSTB isoforms were identified in patients with progressive myoclonus epilepsy of Unverricht-Lundborg type . PMID: 17003839
    36. Results describe the influence of pH and trifluoroethanol on amyloid fibril growth and morphology from human stefin B. PMID: 17701471
    37. cystatin B in vivo has a polymeric structure sensitive to the redox environment and that overexpression of the protein generates aggregates. PMID: 17920138
    38. CSTB is specifically overexpressed in most HCCs and is also elevated in the serum of a large proportion of HCC patients PMID: 18281540
    39. Data show that wild-type stefin B and its Y31 isoform are able to form pores in planar lipid bilayers, whereas the G4R isoform destroys the bilayer by a non pore-forming process. PMID: 18397316
    40. The mechanism of amyloid-fibril formation by stefin B: temperature and protein concentration dependence of the rates;the observed kinetics follow the nucleation and growth behavior observed for many other amyloidogenic proteins. PMID: 18636508
    41. potential role for CSTB in HIV replication in placental macrophages PMID: 18951626
    42. cystatin B interacts with STAT-1 and the levels of STAT-1 tyrosine phosphorylation (but not serine phosphorylation) between uninfected and HIV-infected PM and MDM are differentially regulated. PMID: 19342095

    顯示更多

    收起更多

  • 相關疾病:
    Epilepsy, progressive myoclonic 1 (EPM1)
  • 亞細胞定位:
    Cytoplasm. Nucleus.
  • 蛋白家族:
    Cystatin family
  • 數據庫鏈接:

    HGNC: 2482

    OMIM: 254800

    KEGG: hsa:1476

    STRING: 9606.ENSP00000291568

    UniGene: Hs.695



主站蜘蛛池模板: 久久中文字幕无码a片不卡古代 | 亚洲精品成人av在线观看爽翻天| 亚洲欧洲日产国产 最新| av区无码字幕中文色| 成·人免费午夜无码视频蜜芽| 一区二三区在线 | 中国| 熟妇的奶头又大又粗视频| 人人妻人人澡av天堂香蕉| 中文字幕乱码熟女人妻水蜜桃| 国产色爱av资源综合区| 久久天天躁狠狠躁夜夜avapp| 45分钟免费真人视频| 人妻人人做人做人人爱| 久久久久久久女国产乱让韩 | 亚洲乱码在线卡一卡二卡新区| 亚洲欧美日韩在线一区| 久久久久久成人毛片免费看| 久久人人爽人人爽人人片av高请| 国产成人无码国产亚洲| 天天拍夜夜添久久精品| 国产免费午夜福利不卡片在线| 国产麻豆精品福利在线观看| 亚洲中文字幕久久精品无码喷水 | 色依依av在线| 51久久成人国产精品麻豆| 怡春院国产精品视频| av无码电影一区二区三区| 久热这里只有精品99在线观看 | 国产亚洲欧美另类一区二区 | 少妇spa推油被扣高潮| 激烈的性高湖波多野结衣 | 亚洲欧洲精品a片久久99| 国产精品禁18久久久夂久| 中文字幕人妻丝袜二区| 天天躁日日躁狠狠躁av麻豆男男| 7777精品伊人久久久大香线蕉| 久久久中文字幕日本无吗| 亚洲处破女av日韩精品波波网| 亚洲乱码卡一卡二卡新区仙踪木木| 性饥渴少妇av无码毛片| 欧美日韩综合一区二区三区|