在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

CDC45 Antibody

  • 中文名稱:
    CDC45兔多克隆抗體
  • 貨號:
    CSB-PA005020ESR2HU
  • 規格:
    ¥440
  • 圖片:
    • Western Blot
      Positive WB detected in: 293T whole cell lysate, JK whole cell lysate, MCF7 whole cell lysate, PC-3 whole cell lysate
      All lanes: CDC45 antibody at 1:500
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 66 kDa
      Observed band size: 66 kDa
    • Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA005020ESR2HU at dilution of 1:100
    • Immunoprecipitating CDC45 in K562 whole cell lysate
      Lane 1: Rabbit control IgG instead of (1μg) instead of CSB-PA005020ESR2HU in K562 whole cell lysate.For western blotting, a HRP-conjugated light chain specific antibody was used as the Secondary antibody (1/50000)
      Lane 2: CSB-PA005020ESR2HU (4μg) + K562 whole cell lysate (500μg)
      Lane 3: K562 whole cell lysate (20μg)
    • Immunofluorescent analysis of Hela cells using CSB-PA005020ESR2HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
  • 其他:

產品詳情

  • 產品名稱:
    Rabbit anti-Homo sapiens (Human) CDC45 Polyclonal antibody
  • Uniprot No.:
  • 基因名:
  • 別名:
    CDC 45 antibody; CDC 45L antibody; CDC45 (cell division cycle 45; S.cerevisiae homolog) like antibody; CDC45 antibody; CDC45 cell division cycle 45 like antibody; CDC45 like antibody; CDC45 related protein antibody; CDC45_HUMAN antibody; CDC45L2 antibody; Cell division control protein 45 homolog antibody; Cell division cycle 45 like 2 antibody; Cell division cycle 45 like antibody; PORC PI 1 antibody; PORC-PI-1 antibody
  • 宿主:
    Rabbit
  • 反應種屬:
    Human
  • 免疫原:
    Recombinant Human Cell division control protein 45 homolog protein (1-270AA)
  • 免疫原種屬:
    Homo sapiens (Human)
  • 標記方式:
    Non-conjugated
  • 克隆類型:
    Polyclonal
  • 抗體亞型:
    IgG
  • 純化方式:
    Antigen Affinity Purified
  • 濃度:
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液:
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • 產品提供形式:
    Liquid
  • 應用范圍:
    ELISA, WB, IHC, IP, IF
  • 推薦稀釋比:
    Application Recommended Dilution
    WB 1:1000-1:5000
    IHC 1:20-1:200
    IP 1:200-1:2000
    IF 1:50-1:200
  • Protocols:
  • 儲存條件:
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期:
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途:
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能:
    Required for initiation of chromosomal DNA replication.
  • 基因功能參考文獻:
    1. The size and relative position of the three helices (alpha2, alpha7 and alpha8) that contribute to the active site architecture are highly conserved between Cdc45 and RecJ, whereas the helical structure on the solvent-exposed DHH side (alpha3, alpha4 and alpha5) shows more conformational variability. PMID: 27189187
    2. strong evidence that Cdc45 directly loads RPA on the emerging nascent ssDNA PMID: 28100698
    3. The oncogenic activity of cell division cycle 45 was also confirmed in vivo. In conclusion, cell division cycle 45 may serve as a novel biomarker and a potential therapeutic target for papillary thyroid cancer. PMID: 28474999
    4. Mutations in CDC45, Encoding an Essential Component of the Pre-initiation Complex, Cause Meier-Gorlin Syndrome and Craniosynostosis PMID: 27374770
    5. ectopic expression of Cdc45 led to increased firing of replication origins, severe replication stress, an early S-phase arrest, replication fork stalling, and eventually cell death by apoptosis. PMID: 26919204
    6. The depletion of HDHB from human cells diminishes Cdc45 association with chromatin, suggesting that HDHB may facilitate Cdc45 recruitment at G1/S in human cells. PMID: 25933514
    7. The findings suggest that hCdc45 not only binds to but also slides on DNA with a 3'-5' polarity and, thereby acts as a molecular 'wedge' to initiate DNA strand displacement. PMID: 24293646
    8. Data suggest that CDC45 and MCM10 (minichromosome maintenance complex component 10) directly interact and establish a mutual co-operation in DNA binding; key domains appear to interact and then interact with DNA inside cells or in cell-free systems. PMID: 23750504
    9. CDC45 directly interact with MCM2-7 proteins; with PSF2, PSF3 and SLD5 in GINS subunits; and with replication protein A2, AND-1. A considerable portion of CDC45 localizes in a region other than the DNA replication forks in nuclei. PMID: 23364835
    10. After UV-damage, Cdc45 is still present in a large multi-protein complex and that its mobility within living cells is consistently similar following UVC-mediated DNA damage. PMID: 22536402
    11. Data show that using a 200-nt primed circular DNA substrate, the combined action of DNA polymerase epsilon and the Cdc45/Mcm2-7/GINS (CMG complex) leads to the formation of products >10 kb in length. PMID: 22474384
    12. Structural and functional insights into the DNA replication factor Cdc45 reveal an evolutionary relationship to the DHH family of phosphoesterases PMID: 22147708
    13. Cdc45, which is known to be required for replication fork progression, shows similar patterns of origin association to those of Timeless PMID: 20124417
    14. The coordinated binding of DUE-B and Cdc45 to origins and the physical interactions of DUE-B, Cdc45, and TopBP1 suggest that complexes of these proteins are necessary for replication initiation. PMID: 20065034
    15. the Chk1-mediated S-phase checkpoint targets initiation factor Cdc45 via a Cdc25A/Cdk2-independent mechanism PMID: 16912045
    16. CDC45L is part of the GINS complex. PMID: 17557111
    17. Cdc45 may play an important role in elongation of DNA replication by bridging the processive DNA polymerases delta and epsilon with the replicative helicase in the elongating machinery PMID: 17573775
    18. Cdc45 expression is tightly associated with proliferating cell populations and Cdc45 seems to be a promising candidate for a novel proliferation marker in cancer cell biology PMID: 17608804
    19. Thus, the evidences for ubiquitylation of Cdc45 refer the first posttranslational modification of this essential replication factor. PMID: 17767920
    20. human TopBP1 is involved in the formation of the initiation complex of replication in human cells and is required for the recruitment of Cdc45 to origins of DNA replication PMID: 17887956
    21. Results suggest that apoptosis induced by replication inhibitors in Chk1-depleted cells is dependent upon the helicase cofactor Cdc45. PMID: 18239674
    22. CDT1 associates with CDC7 and recruits CDC45 to chromatin during S phase PMID: 19054765
    23. interactions between Cdc45, Mcm2-7, and the GINS complex (collectively called the CMG complex), which seem to play a key role in the formation and progression of replication forks PMID: 19805216

    顯示更多

    收起更多

  • 亞細胞定位:
    Cytoplasm. Nucleus.
  • 蛋白家族:
    CDC45 family
  • 組織特異性:
    Widely expressed, highest levels are found in adult testis and thymus and in fetal liver.
  • 數據庫鏈接:

    HGNC: 1739

    OMIM: 603465

    KEGG: hsa:8318

    UniGene: Hs.474217



主站蜘蛛池模板: 波多野结衣乳巨码无在线观看| 熟妇人妻va精品中文字幕| 免费人成视频x8x8| 色老久久精品偷偷鲁| 亚洲 另类 日韩 制服 无码| 狠狠躁夜夜躁人人躁婷婷| 超级碰97直线国产免费公开| 粉嫩小泬视频无码视频软件| 成人亚洲a片v一区二区三区动漫 | 看曰本女人大战黑人视频| 海角社区在线视频播放观看| 久久久久久99av无码免费网站| 国产精品va在线观看老妇女| 国产精品无码午夜免费影院| 亚洲国产成人无码网站大全| 久久96国产精品久久久| 国产在线看片免费视频| 久久www免费人成看片入口| 国产午夜亚洲精品不卡下载| 国产精品538一区二区在线| 国产午夜成人无码免费看不卡| 猫咪免费人成网站在线观看| 午夜丰满少妇性开放视频| 韩国乱码伦视频免费| 一本一道精品欧美中文字幕| 噜噜噜av久久| 日本中文一区二区三区亚洲| 无码中文字幕日韩专区视频| 开心五月综合亚洲| 久久人人爽av亚洲精品| 久久婷婷成人综合色| 亚洲成a人片在线不卡一二三区 | 久久久亚洲欧洲日产国码aⅴ| 人人妻人人爽人人做夜欢视频| 国产香蕉尹人在线视频你懂的| 国产农村乱辈无码| 潮喷失禁大喷水aⅴ无码| 熟女人妻在线视频| 专干熟肥老妇人视频在线看| av无码免费岛国动作片不卡| 狠狠色噜噜狠狠狠狠av|