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  4. ATP6V1G1 Antibody

ATP6V1G1 Antibody

  • 中文名稱：
    ATP6V1G1兔多克隆抗體
  • 貨號：
    CSB-PA00135A0Rb
  • 規格：
    ￥440
  • 圖片：
    • Western blot
      All lanes: V-type proton ATPase subunit G 1 antibody at 2μg/ml + 293T whole cell lysate
      Secondary
      Goat polyclonal to rabbit IgG at 1/15000 dilution
      Predicted band size: 14 kDa
      Observed band size: 14 kDa
    • Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA00135A0Rb at dilution of 1:50
    • Immunofluorescence staining of PC-3 cells with CSB-PA00135A0Rb at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA00135A0Rb at dilution of 1:100
  • 其他：

產品詳情

  • 產品名稱：
    Rabbit anti-Homo sapiens (Human) ATP6V1G1 Polyclonal antibody
  • Uniprot No.：
  • 基因名：
  • 別名：
    ATP 6G antibody; ATP 6J antibody; ATP6G antibody; ATP6G1 antibody; ATP6GL antibody; Atp6v1g1 antibody; ATPase H+ transporting lysosomal (vacuolar proton pump) member J antibody; ATPase H+ transporting lysosomal 13kD V1 subunit G isoform 1 antibody; ATPase H+ transporting lysosomal 13kDa V1 subunit G1 antibody; ATPase H+ transporting lysosomal member J antibody; ATPase H+ transporting lysosomal subunit G isoform 1 antibody; DKFZp547P234 antibody; V ATPase 13 kDa subunit 1 antibody; V ATPase G subunit 1 antibody; V-ATPase 13 kDa subunit 1 antibody; V-ATPase subunit G 1 antibody; V-type proton ATPase subunit G 1 antibody; Vacuolar ATP synthase subunit G 1 antibody; Vacuolar ATP synthase subunit G1 antibody; Vacuolar ATP synthase subunit M16 antibody; Vacuolar H+ ATPase G1 antibody; Vacuolar H+ ATPase subunit G 1 antibody; Vacuolar proton pump G subunit 1 antibody; Vacuolar proton pump subunit G 1 antibody; Vacuolar proton pump subunit M16 antibody; VATG1_HUMAN antibody; Vma 10 antibody; Vma10 antibody
  • 宿主：
    Rabbit
  • 反應種屬：
    Human
  • 免疫原：
    Recombinant Human V-type proton ATPase subunit G 1 protein (2-118AA)
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated

    本頁面中的產品，ATP6V1G1 Antibody (CSB-PA00135A0Rb)，的標記方式是Non-conjugated。對于ATP6V1G1 Antibody，我們還提供其他標記。見下表：

    可提供標記
    標記方式 貨號 產品名稱 應用
    HRP CSB-PA00135B0Rb ATP6V1G1 Antibody, HRP conjugated ELISA
    FITC CSB-PA00135C0Rb ATP6V1G1 Antibody, FITC conjugated
    Biotin CSB-PA00135D0Rb ATP6V1G1 Antibody, Biotin conjugated ELISA
  • 克隆類型：
    Polyclonal
  • 抗體亞型：
    IgG
  • 純化方式：
    >95%, Protein G purified
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB, IHC, IF
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:1000-1:5000
    IHC 1:20-1:200
    IF 1:50-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    Subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment. In aerobic conditions, involved in intracellular iron homeostasis, thus triggering the activity of Fe(2+) prolyl hydroxylase (PHD) enzymes, and leading to HIF1A hydroxylation and subsequent proteasomal degradation.
  • 基因功能參考文獻：
    1. RILP regulates vacuolar ATPase through interaction with the V1G1 subunit PMID: 26180254
    2. genome-wide association study in population in China: Data suggest 4 SNPs upstream of ATP6V1G1 (rs10817638, rs7851259, rs10982287, rs4979427) are associated (in bivariate manner) with age at menarche and bone density. [includes META-ANALYSIS] PMID: 26312577
    3. RILP regulates the activity of the V-ATPase through its interaction with V1G1. PMID: 24762812
  • 亞細胞定位：
    Apical cell membrane.
  • 蛋白家族：
    V-ATPase G subunit family
  • 組織特異性：
    Kidney; localizes to early distal nephron, encompassing thick ascending limbs and distal convoluted tubules (at protein level). Ubiquitous.
  • 數據庫鏈接：

    HGNC: 864

    OMIM: 607296

    KEGG: hsa:9550

    STRING: 9606.ENSP00000363162

    UniGene: Hs.388654



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