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  4. AFG1L Antibody

AFG1L Antibody

  • 中文名稱：
    AFG1L兔多克隆抗體
  • 貨號：
    CSB-PA819895LA01HU
  • 規格：
    ￥440
  • 圖片：
    • Western Blot
      Positive WB detected in: A549 whole cell lysate, Rat kidney tissue
      All lanes: AFG1L antibody at 3µg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 55 kDa
      Observed band size: 55 kDa
    • IHC image of CSB-PA819895LA01HU diluted at 1:600 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • IHC image of CSB-PA819895LA01HU diluted at 1:600 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of HepG2 cells with CSB-PA819895LA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

產品詳情

  • 產品名稱：
    Rabbit anti-Homo sapiens (Human) AFG1L Polyclonal antibody
  • Uniprot No.：
  • 基因名：
    AFG1L
  • 別名：
    AFG1 antibody; ATPase family gene 1 homolog antibody; CG8520 gene product antibody; lace1 antibody; LACE1_HUMAN antibody; Lactation elevated protein 1 antibody; Lactivation elevated 1 antibody; Protein AFG1 homolog antibody
  • 宿主：
    Rabbit
  • 反應種屬：
    Human, Rat
  • 免疫原：
    Recombinant Human AFG1-like ATPase protein (14-313AA)
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated

    本頁面中的產品，AFG1L Antibody (CSB-PA819895LA01HU)，的標記方式是Non-conjugated。對于AFG1L Antibody，我們還提供其他標記。見下表：

    可提供標記
    標記方式 貨號 產品名稱 應用
    HRP CSB-PA819895LB01HU AFG1L Antibody, HRP conjugated ELISA
    FITC CSB-PA819895LC01HU AFG1L Antibody, FITC conjugated
    Biotin CSB-PA819895LD01HU AFG1L Antibody, Biotin conjugated ELISA
  • 克隆類型：
    Polyclonal
  • 抗體亞型：
    IgG
  • 純化方式：
    >95%, Protein G purified
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB, IHC, IF
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:500-1:1000
    IF 1:200-1:500
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    Putative mitochondrial ATPase. Plays a role in mitochondrial morphology and mitochondrial protein metabolism. Promotes degradation of excess nuclear-encoded complex IV subunits (COX4I1, COX5A and COX6A1) and normal activity of complexes III and IV of the respiratory chain. Mediates mitochondrial translocation of TP53 and its transcription-independent apoptosis in response to genotoxic stress.
  • 基因功能參考文獻：
    1. Increased expression of LACE1 partitions p53 to mitochondria, causes reduction in nuclear p53 content and induces apoptosis. Thus, LACE1 mediates mitochondrial translocation of p53 and its transcription-independent apoptosis. PMID: 27323408
    2. This study establishes LACE1 as a novel factor with a crucial role in mitochondrial protein homeostasis. PMID: 26759378
    3. association between bipolar disorder and two SNPs in the gene LACE1 PMID: 20872768
    4. Observational study of gene-disease association. (HuGE Navigator) PMID: 20877624
    5. Observational study of gene-disease association. (HuGE Navigator) PMID: 19913121
    6. Observational study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator) PMID: 20628086
    7. Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator) PMID: 20379614
    8. Observational study of genetic testing. (HuGE Navigator) PMID: 18237552

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  • 亞細胞定位：
    Mitochondrion membrane.
  • 蛋白家族：
    AFG1 ATPase family
  • 數據庫鏈接：

    HGNC: 16411

    OMIM: 617469

    KEGG: hsa:246269

    STRING: 9606.ENSP00000357973

    UniGene: Hs.259666



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