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ACAA2 Antibody

  • 中文名稱：
    ACAA2兔多克隆抗體
  • 貨號：
    CSB-PA001118LA01HU
  • 規格：
    ￥440
  • 圖片：
    • Western Blot
      Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, NIH/3T3 whole cell lysate, HepG2 whole cell lysate, Rat lung tissue, Mouse liver tissue, Mouse lung tissue
      All lanes: ACAA2 antibody at 2.4μg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 42 kDa
      Observed band size: 42 kDa
    • IHC image of CSB-PA001118LA01HU diluted at 1:700 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescent analysis of HepG2 cells using CSB-PA001118LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
    • Immunoprecipitating ACAA2 in A549 whole cell lysate
      Lane 1: Rabbit control IgG (1ug) instead of CSB-PA001118LA01HU in A549 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
      Lane 2: CSB-PA001118LA01HU (6ug) + A549 whole cell lysate (500ug)
      Lane 3: A549 whole cell lysate (10ug)
  • 其他：

產品詳情

  • 產品描述：

    To yield the ACAA2 polyclonal antibody, the recombinant human ACAA2 protein (188-257aa) is used to immunize a rabbit. The rabbit's immune system will recognize the immunogen as foreign and produce antibodies against it. After several rounds of immunization, blood is collected from the rabbit and the serum is isolated. The ACAA2 polyclonal antibodies are purified from the rabbit's serum using protein G affinity chromatography. The purity of this ACAA2 antibody is over 95%. This ACAA2 antibody can be used to detect human, mouse, and rat ACAA2 proteins in multiple applications, including ELISA, WB, IHC, IF, and IP.

    The ACAA2 protein is an enzyme involved in fatty acid metabolism. Its main function is to catalyze the final step in the beta-oxidation of fatty acids. This is a process by which the body breaks down fatty acids for energy production, particularly during periods of fasting or prolonged exercise when glucose levels are low. ACAA2 also plays a role in the regulation of lipid metabolism and insulin sensitivity. Mutations in the ACAA2 gene can lead to metabolic disorders, such as obesity and insulin resistance.

  • 產品名稱：
    Rabbit anti-Homo sapiens (Human) ACAA2 Polyclonal antibody
  • Uniprot No.：
  • 基因名：
    ACAA2
  • 別名：
    3 ketoacyl CoA thiolase antibody; 3 ketoacyl CoA thiolase mitochondrial antibody; 3-ketoacyl-CoA thiolase, mitochondrial antibody; 3-oxoacyl-CoA thiolase, mitochondrial antibody; ACAA 2 antibody; Acaa2 antibody; Acetyl CoA acyltransferase 2 antibody; Acetyl CoA acyltransferase antibody; Acetyl Coenzyme A acyltransferase 2 antibody; Acetyl-CoA acyltransferase antibody; Beta ketothiolase antibody; Beta-ketothiolase antibody; DSAEC antibody; Mitochondrial 3 oxoacyl CoA thiolase antibody; Mitochondrial 3 oxoacyl Coenzyme A thiolase antibody; Mitochondrial 3-oxoacyl-CoA thiolase antibody; T1 antibody; THIM_HUMAN antibody
  • 宿主：
    Rabbit
  • 反應種屬：
    Human, Mouse, Rat
  • 免疫原：
    Recombinant Human 3-ketoacyl-CoA thiolase, mitochondrial protein (188-257AA)
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated

    本頁面中的產品，ACAA2 Antibody (CSB-PA001118LA01HU)，的標記方式是Non-conjugated。對于ACAA2 Antibody，我們還提供其他標記。見下表：

    可提供標記
    標記方式 貨號 產品名稱 應用
    HRP CSB-PA001118LB01HU ACAA2 Antibody, HRP conjugated ELISA
    FITC CSB-PA001118LC01HU ACAA2 Antibody, FITC conjugated
    Biotin CSB-PA001118LD01HU ACAA2 Antibody, Biotin conjugated ELISA
  • 克隆類型：
    Polyclonal
  • 抗體亞型：
    IgG
  • 純化方式：
    >95%, Protein G purified
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB, IHC, IF, IP
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:1000-1:5000
    IHC 1:500-1:1000
    IF 1:50-1:500
    IP 1:200-1:2000
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    In the production of energy from fats, this is one of the enzymes that catalyzes the last step of the mitochondrial beta-oxidation pathway, an aerobic process breaking down fatty acids into acetyl-CoA. Using free coenzyme A/CoA, catalyzes the thiolytic cleavage of medium- to long-chain unbranched 3-oxoacyl-CoAs into acetyl-CoA and a fatty acyl-CoA shortened by two carbon atoms. Also catalyzes the condensation of two acetyl-CoA molecules into acetoacetyl-CoA and could be involved in the production of ketone bodies. Also displays hydrolase activity on various fatty acyl-CoAs. Thereby, could be responsible for the production of acetate in a side reaction to beta-oxidation. Abolishes BNIP3-mediated apoptosis and mitochondrial damage.
  • 基因功能參考文獻：
    1. ACAA2 is a functional BNIP3 binding partner and provide a possible linkage between fatty acid metabolism and apoptosis of cells. PMID: 18371312
  • 亞細胞定位：
    Mitochondrion.
  • 蛋白家族：
    Thiolase family
  • 數據庫鏈接：

    HGNC: 83

    OMIM: 604770

    KEGG: hsa:10449

    STRING: 9606.ENSP00000285093

    UniGene: Hs.200136



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