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  4. CANX Monoclonal Antibody

CANX Monoclonal Antibody

  • 中文名稱：
    CANX鼠單克隆抗體
  • 貨號：
    CSB-MA004485A1m
  • 規格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in: MCF7 whole cell lysate, U87 whole cell lysate, A549 whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate, U251 whole cell lysate, K562 whole cell lysate
      All lanes: CANX antibody at 1:2000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 68, 72, 56 kDa
      Observed band size: 90 KDa
      Exposure time：1min
    • Immunofluorescence staining of Hela cells with CANX CSB-MA004485A1m at 1:140, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
    • Immunofluorescence staining of HepG2 cells with CANX CSB-MA004485A1m at 1:140, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
    • Overlay Peak curve showing Hela cells stained with CSB-MA004485A1m (red line) at 1:140. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1μg/1*106cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1μg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

產品詳情

  • 產品描述：
    CANX單克隆抗體是特異性識別人類Calnexin（CANX）的科研用免疫試劑，該靶標為內質網關鍵分子伴侶蛋白，參與新生糖蛋白折疊質量控制及內質網應激調控，在細胞穩態維持、腫瘤微環境調控及神經退行性疾病機制研究中具有重要科學價值。本產品通過雜交瘤技術制備，經ELISA、Western Blot、免疫熒光及流式細胞術多平臺驗證，展現高親和力與低交叉反應性，適用于檢測內源性CANX蛋白表達及亞細胞定位分析。在腫瘤細胞應激反應研究、神經退行性疾病模型構建、免疫細胞功能調控等基礎科研領域具有廣泛應用，特別適用于探索內質網-線粒體交互作用、未折疊蛋白反應通路及自噬相關機制等前沿課題。該抗體滿足細胞裂解液、固定組織樣本等多種實驗體系的檢測需求，為蛋白質相互作用研究、信號轉導通路解析提供可靠工具。
  • 產品名稱：
    Mouse anti-Homo sapiens (Human) CANX Monoclonal antibody
  • Uniprot No.：
  • 基因名：
  • 別名：
    Calnexin antibody; CALX_HUMAN antibody; CANX antibody; CNX antibody; FLJ26570 antibody; Histocompatibility complex class I antigen binding protein p88 antibody; IP90 antibody; Major histocompatibility complex class I antigen-binding protein p88 antibody; p90 antibody
  • 宿主：
    Mouse
  • 反應種屬：
    Human
  • 免疫原：
    Recombinant Human Calnexin protein (1-482AA)
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    IgG2b
  • 純化方式：
    >95%, Protein G purified
  • 克隆號：
    15B1A7
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB, IF, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB WB：1:1000-1:5000
    IF 1:50-1:200
    FC 1:50-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    Calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. Associated with partial T-cell antigen receptor complexes that escape the ER of immature thymocytes, it may function as a signaling complex regulating thymocyte maturation. Additionally it may play a role in receptor-mediated endocytosis at the synapse.
  • 基因功能參考文獻：
    1. In summary, ER retention of pathogenic VLDLR mutants involves binding to calnexin, elevated endoplasmic reticulum stress, and delayed degradation which is dependent on SEL1L. PMID: 29371607
    2. Increased tumour protein levels of calnexin may be of prognostic significance in colorectal cancer, and calnexin may represent a potential target for future therapies. PMID: 27369741
    3. FUNDC1 integrates mitochondrial fission and mitophagy at the interface of the endoplasmic reticulum-mitochondrial contact site by working in concert with DRP1 and calnexin under hypoxic conditions in mammalian cells. PMID: 27145933
    4. Results of further analyses by using a CNX mutant imply that ERp29 and ERp57 recognize the same domain of CNX, whereas the mode of interaction with CNX might be somewhat different between them. PMID: 28456374
    5. Inhibit interaction between HIV-1 Nef and Calnexin to reverse HIV-induced lipid accumulation and prevent atherosclerosis. PMID: 27470515
    6. Endogenous NOX4 forms macromolecular complexes with calnexin, which are needed for the proper maturation, processing, and function of NOX4 in the endoplasmic reticulum. PMID: 26861875
    7. To study principles underlying dynamics and regulation of palmitoylation, the ER chaperone calnexin, which requires dual palmitoylation for function, was investigated. PMID: 26900856
    8. These observations point at a previously unrecognized contribution of calnexin to the retention of NMT1 at the ER membrane. PMID: 26603938
    9. Charcot-Marie-Tooth disease-related PMP22 is trapped in the endoplasmic reticulum by calnexin-dependent retention and Rer1-mediated early Golgi retrieval systems and partly degraded by the Hrd1-mediated endoplasmic reticulum-associated degradation system. PMID: 25385046
    10. Data indicate that protein tyrosine phosphatase 1B (PTP1B) association with calnexin is ubiquitin conjugating enzyme 9 (UBC9)-dependent. PMID: 25586181
    11. H-ERG trafficking was impaired by H2O2 after 48 h treatment, accompanied by reciprocal changes of expression between miR-17-5p seed miRNAs and several chaperones (Hsp70, Hsc70, CANX, and Golga20) PMID: 24386440
    12. Nef regulates the activity of calnexin to stimulate its interaction with gp160 at the expense of ABCA1 PMID: 25170080
    13. The impact of CANX genetic variants to heart rate was discovered. PMID: 24680774
    14. Soluble calnexin may fulfill functions similar to calreticulin. PMID: 24056258
    15. calnexin tunes the cellular responses to epidermal growth factor receptor in a manner that depends on the health status of the endoplasmic reticulum PMID: 23932718
    16. These findings demonstrated that calnexin strictly monitors the maturation of S protein by its direct binding, resulting in conferring infectivity on severe acute respiratory syndrome coronavirus. PMID: 22915798
    17. The data suggest that patients with low or defective TAP1 or calnexin in primary breast cancers may be at higher risks for developing brain metastasis due to the defects in T cell-based immunosurveillance. PMID: 22065046
    18. cysteine residues within calnexin are important for the structure and function of calnexin. PMID: 21842374
    19. The findings showed that calnexin is a stable component of the ribosome-translocon complex in a manner that is exquisitely dependent on calnexin's palmitoylation status. PMID: 22314232
    20. HLA-I, TAP1, CNX, LMP7, Erp57, Tapasin and ERAP1 were down-regulated in 68%, 44%, 48%, 40%, 52%, 32% and 20% of esophageal squamous cell carcinoma lesions then, respectively. PMID: 21362330
    21. Nixin/ZNRF4 to be central for the regulation of calnexin turnover. PMID: 21205830
    22. Results reveal the P-domain functions as a unique protein-protein interaction domain and implicate a peptidyl prolyl isomerase as a new element in the calnexin cycle. PMID: 20801878
    23. calnexin could bind PrP both in vitro and in vivo; calnexin prevents caspase-3-mediated cytotoxicity induced by PrP PMID: 20506117
    24. Transmembrane segments prevent surface expression of sodium channel Nav1.8 and promote calnexin-dependent channel degradation PMID: 20720009
    25. human delta opioid receptor (hdeltaOR) exists in a ternary complex with SERCA2b and the ER molecular chaperone calnexin PMID: 20528919
    26. Env interacts with intracellular CNX and extracellular PDI via discrete, largely nonoverlapping, regions. PMID: 20202930
    27. Results show that the phosphorylation of calnexin is linked to the efficiency of secretion of the cargo glycoprotein, in this case, alpha1-antitrypsin. PMID: 19815548
    28. Data show that demonstrate that Cnx preferentially associates with misfolded mutant opsins associated with retinitis pigmentosa. PMID: 19801547
    29. EDEM appeared to function in the ERAD (endoplasmic reticulum-associated degradation)pathway by accepting substrates from calnexin. PMID: 12610305
    30. EDEM was shown to extract misfolded glycoproteins, but not glycoproteins undergoing productive folding, from the calnexin cycle PMID: 12610306
    31. Data show that calnexin associates with newly synthesized proteolipid protein (PLP) molecules, binding stably to misfolded PLP. PMID: 12805210
    32. calnexin-downregulation may contribute to the metastatic phenotype of melanoma cells in vivo PMID: 14732231
    33. Calnexin provides long-term protection of wild-type Shaker protein from ER-associated degradation. PMID: 15161937
    34. the contribution of both the b and b' domains to the binding with CNX and calreticulin was revealed PMID: 15236594
    35. results support emerging models for a glycan-independent chaperone role for calnexin and for the mechanism of retention of misfolded membrane proteins in the endoplasmic reticulum PMID: 15537650
    36. Calnexin decreases with aging and might contribute to a cytoprotection in a variety of human age-related diseases. PMID: 15557823
    37. Data show that the major degradation pathway of the cystic fibrosis transmembrane conductance regulator with F508 deletion from the endoplasmic reticulum is independent of calnexin. PMID: 15923638
    38. Calnexin associates with the neonatal Fc receptor for IgG (FcRn) heavy chain before it associates noncovalently with beta 2-microglobulin. PMID: 16002696
    39. Polypeptide substrate recognition by CANX requires specific conformations of the CANX protein. PMID: 16061483
    40. We characterized a molecular mechanism by which calnexin regulates ER-stress-mediated apoptosis in a manner independent of its chaperone functions but dependent of its binding to Bap31. PMID: 16858427
    41. Here, we have observed that NCT N-linked oligosaccharides mediated specific interactions with the secretory pathway lectins calnexin and ERGIC-53 PMID: 16938437
    42. proportion of the human and the rat WT gonadotropin-releasing hormone receptor appears to be retained in the endoplasmic reticulum by calnexin, an effect that decreases GnRHR signaling capacity PMID: 17170088
    43. These results suggest that MCF-7 resistance to endoplasmic reticulum stress-induced apoptosis is partially mediated by the expression level of calnexin which in turn controls its sub-cellular localization, and its association with Bap31. PMID: 17203246
    44. D1 and D2 dopamine receptor expression is regulated by direct interaction with the chaperone protein calnexin PMID: 17395585
    45. Endoplasmic reticulum chaperones stabilize nicotinic receptor subunits and regulate receptor assembly. PMID: 17728248
    46. Interaction with calnexin led to accumulation of GAT1 in concentric bodies corresponding to previously described multilamellar ER-derived structures. PMID: 18367207
    47. the phosphorylation state of the calnexin cytosolic domain and its interaction with PACS-2 sort the chaperone between domains of the ER and the plasma membrane PMID: 18417615
    48. A dependence on calnexin for proper assembly of CFTR's membrane spanning domains, was identified. PMID: 18716059
    49. ERp57 must be physically associated with the calnexin cycle to catalyze isomerization reactions with most of its substrates PMID: 19054761
    50. alphaIIb interacts with calnexin via its N15-linked glycan, and alphaIIbbeta3 biogenesis is partially controlled by engagement of alphaIIb in the calnexin cycle. PMID: 16304048

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  • 亞細胞定位：
    Endoplasmic reticulum membrane; Single-pass type I membrane protein. Endoplasmic reticulum. Melanosome.
  • 蛋白家族：
    Calreticulin family
  • 數據庫鏈接：

    HGNC: 1473

    OMIM: 114217

    KEGG: hsa:821

    STRING: 9606.ENSP00000247461

    UniGene: Hs.567968



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