ABCB1 Monoclonal Antibody
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中文名稱：
ABCB1鼠單克隆抗體
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貨號：
CSB-MA001046A0m
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規格：
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圖片：
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Western Blot
Positive WB detected in: HepG2 whole cell lysate, 293 whole cell lysate, Hela whole cell lysate, SY5Y whole cell lysate, U251 whole cell lysate
All lanes: ABCB1 antibody at 1:1000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 141 kDa
Observed band size: 141-250 KDa
Exposure time：5min
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Immunofluorescence staining of HepG2 cells with CSB-MA001046A0m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
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Immunofluorescence staining of Hela cells with CSB-MA001046A0m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
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Overlay Peak curve showing Hela cells stained with CSB-MA001046A0m (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1μg/1*106cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1μg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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其他：
產品詳情
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產品描述：
ABCB1單克隆抗體（CUSABIO貨號：CSB-MA001046A0m）是針對人源ABCB1/P-糖蛋白研發的小鼠源IgG1 Kappa型單克隆抗體，適用于ELISA、Western Blot、免疫熒光（IF）及流式細胞術（FC）等多種實驗場景。ABCB1基因編碼的P-糖蛋白屬于ATP結合盒（ABC）轉運蛋白超家族成員，廣泛分布于腫瘤細胞膜及血腦屏障內皮細胞，其過表達可通過外排化療藥物介導腫瘤多藥耐藥性，是癌癥治療及藥物代謝研究的關鍵靶點。本產品采用重組人源ABCB1蛋白片段（NP_000918, 620-709氨基酸區域）作為免疫原，經雜交瘤技術制備而成，具有高特異性識別能力，保存條件為-20°C以下分裝避光儲存。該抗體可助力于腫瘤耐藥機制解析、藥物轉運蛋白功能研究及血腦屏障調控等基礎科研領域，為探索ABCB1在細胞內外源性物質代謝中的分子機制提供可靠工具。
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產品名稱：
Mouse anti-Homo sapiens (Human) ABCB1 Monoclonal antibody
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Uniprot No.：
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基因名：
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別名：
ABC20 antibody; ABCB1 antibody; ATP binding cassette, sub family B (MDR/TAP), member 1 antibody; ATP-binding cassette sub-family B member 1 antibody; CD243 antibody; CLCS antibody; Colchicin sensitivity antibody; Doxorubicin resistance antibody; GP170 antibody; MDR1 antibody; MDR1_HUMAN antibody; Multidrug resistance 1 antibody; Multidrug resistance protein 1 antibody; P glycoprotein 1 antibody; P gp antibody; P-glycoprotein 1 antibody; PGY1 antibody
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宿主：
Mouse
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反應種屬：
Human
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免疫原：
Recombinant Human ATP-dependent translocase ABCB1 protein (350-710AA)
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免疫原種屬：
Homo sapiens (Human)
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標記方式：
Non-conjugated
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克隆類型：
Monoclonal
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抗體亞型：
IgG2b
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純化方式：
>95%, Protein A purified
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克隆號：
5C12D3
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濃度：
It differs from different batches. Please contact us to confirm it.
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保存緩沖液：
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
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產品提供形式：
Liquid
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應用范圍：
ELISA, WB, IF, FC
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推薦稀釋比：
| Application |
Recommended Dilution |
| WB |
WB：1:1000-1:5000 |
| IF |
1:50-1:200 |
| FC |
1:50-1:200 |
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Protocols：
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儲存條件：
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
靶點詳情
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功能：
Translocates drugs and phospholipids across the membrane. Catalyzes the flop of phospholipids from the cytoplasmic to the exoplasmic leaflet of the apical membrane. Participates mainly to the flop of phosphatidylcholine, phosphatidylethanolamine, beta-D-glucosylceramides and sphingomyelins. Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells.
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相關疾病：
Inflammatory bowel disease 13 (IBD13)
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亞細胞定位：
Cell membrane; Multi-pass membrane protein. Apical cell membrane.
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蛋白家族：
ABC transporter superfamily, ABCB family, Multidrug resistance exporter (TC 3.A.1.201) subfamily
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組織特異性：
Expressed in liver, kidney, small intestine and brain.
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數據庫鏈接：
Most popular with customers
Western Blot
Immunofluorescence staining of HepG2 cells with CSB-MA001046A0m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
Immunofluorescence staining of Hela cells with CSB-MA001046A0m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
Overlay Peak curve showing Hela cells stained with CSB-MA001046A0m (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1μg/1*106cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1μg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
