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  4. rat follicle-stimulating hormone,FSH ELISA Kit

rat follicle-stimulating hormone,FSH ELISA Kit

  • 中文名稱：
    大鼠促卵泡素(FSH)酶聯免疫試劑盒
  • 貨號：
    CSB-E06869r
  • 規格：
    96T/48T
  • 價格：
    ￥3900/￥2500
  • 其他：

產品詳情

  • 產品描述：
    大鼠促卵泡素(FSH)酶聯免疫試劑盒（CSB-E06869r）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的FSH含量。FSH（卵泡刺激素）是一種由垂體前葉分泌的激素，對生殖細胞生長和性激素合成起重要作用。其受體FSHR在性腺外組織如破骨細胞、脂肪細胞和神經細胞中表達，通過調節骨量、脂質代謝和認知功能等發揮多種生物學效應。研究顯示，FSH通過FSHR調節胰島素分泌，對維持糖代謝穩態至關重要。生殖內分泌研究和生殖系統疾病模型構建；卵巢功能評估、睪丸發育研究或環境激素干擾實驗；生殖生物學、毒理學及藥理學研究本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    FshbFollitropin subunit beta ELISA kit; Follicle-stimulating hormone beta subunit ELISA kit; FSH-B ELISA kit; FSH-beta ELISA kit; Follitropin beta chain ELISA kit
  • 縮寫：
    FSH
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
  • 靈敏度：
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 本試劑盒不含材料：
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm - 630 nm.
      • An incubator that can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 數據處理：
  • 貨期：
    3-5 working days

引用文獻

產品評價

平均分:
5.0分 - 1 個評價

樣品類型：血清

樣品信息：大鼠

稀釋比：沒有稀釋

產品評價： 我用CSB-E06869r檢測正常(FSH)血清，標準曲線的OD值為0.0838 0.2267 0.5508 0.9712 1.1333 1.3067，樣本的OD值(樣本OD可以提供大概區間)為0.1-0.25。

By 付老師

相關問答

■ 常見問題解答
Q:

樣品稀釋液和洗液的作用相同嗎？對于FSH ELISA試劑盒，怎么稀釋血清？

A:
樣本稀釋液和洗液具有不同的功能，并且它們的成分也不同。 如果你的大鼠血清樣品濃度較高，可以考慮使用樣品稀釋液。但是你應該通過進行預實驗來確定最佳的稀釋倍數。 --建議核對說明書是否有樣本稀釋液，有，則上述不改。

靶點詳情

  • 最新研究進展：
    FSH是垂體前葉分泌的調節生殖系統功能的激素，其在卵巢和睪丸中的作用已被廣泛研究。近期研究發現，FSH的受體在非生殖系統中也存在，并與多種疾病的發病機制相關，如肝纖維化、心血管疾病等。
  • 功能：
    Together with the alpha chain CGA constitutes follitropin, the follicle-stimulating hormone, and provides its biological specificity to the hormone heterodimer. Binds FSHR, a G protein-coupled receptor, on target cells to activate downstream signaling pathways. Follitropin is involved in follicle development and spermatogenesis in reproductive organs.
  • 基因功能參考文獻：
    1. Study in primary rat pituitary cells demonstrates that miR-186-5p regulates FSH secretion by directly targeting the FSHb 3' UTR. PMID: 29534107
    2. our results indicate that miR-21-3p and miR-433 can down-regulate the expression of FSHb by directly targeting the FSHb 3'UTR in rat primary pituitary cells. PMID: 28402262
    3. the upstream region of Fshb is susceptible to unsaturated long-chain fatty acids PMID: 26853521
    4. GnRH stimulation of Fshb expression is dependent on miR-132/212 and involves a SIRT1-FOXO1 pathway. PMID: 25635942
    5. FSH acutely regulated CCND2 expression through both PKA and PI3K signaling pathways during granulosa cell proliferation and also accelerated its ubiquitination-proteasomal degradation. PMID: 23349233
    6. Data suggest that FSH (Cga/FSHb) induces clock system in granulosa cells by up-regulation of connexin 43 expression; this suggests that gap junctions are implicated in circadian clock in peripheral oscillators besides central pacemaker. PMID: 23299500
    7. Both the hormonal milieu of perimenopause and the association of dynamic perimenopausal changes in FSH and inhibin A with decreased bone mineral density have been reproduced in an animal model. PMID: 22249524
    8. FSH autoantibody could cause the reduction of inhibin B, thereby inducing hypospermatogenesis via augment of spermatogenic cell apoptosis PMID: 22564408
    9. Novel findings supporting the role of FSH in primordial follicle survival through an action on apoptosis. PMID: 22106407
    10. These results show that the synthesis of FSH isoforms possessing alpha2,6-linked sialic acid is hormonally regulated in male rats. PMID: 19464343
    11. Data suggest that FSH beta subunit expression (and LH beta subunit expression) is regulated by DUSP1 expression (specifically in response to high-frequency GnRH pulses); this effect may occur in association with MAPK3/1 phosphorylation. PMID: 21228211
    12. A potential new regulatory mechanism of the FSH signalling pathway that could occur during maturation of testicular cells. PMID: 20201104
    13. Pituitary homeobox 1 activates the rat FSHbeta (rFSHbeta) gene through both direct and indirect interactions with the rFSHbeta gene promoter. PMID: 12145338
    14. The regulation of FSHbeta is dependent on activin-mediated signaling factors in concert with pituitary-derived nuclear regulatory proteins. PMID: 12554780
    15. PACAP contributes to the selective regulation of FSHbeta expression during maturation in the male PMID: 12646491
    16. androgens acting directly on the pituitary increase FSH-beta and decrease LH-beta transcription. PMID: 14512439
    17. FSH enhances HIF-1 activity downstream of the PI 3-kinase/AKT/Rheb/mTOR pathway in GCs and HIF-1 activity is necessary for FSH to induce multiple follicular differentiation markers PMID: 14982927
    18. SMAD3 plays an important role in the synergistic effects of activin and gonadotropin-releasing hormone and demonstrate that this synergy is mediated by a palindromic cis-element located at -266/-259 of the rFSHbeta gene promoter PMID: 15375186
    19. Smad3 is not only sufficent but also necessary for activin-mediated regulation of FSHbeta promoter. PMID: 15761025
    20. oxidative stress induces apoptosis in preovulatory follicles and the antiapoptotic effect of FSH is mediated in part by stimulation of follicular GSH synthesis and suppression of ROS production PMID: 16339198
    21. Testosterone regulates FSHbeta transcription, in part, through modulation of various components of the activin-signaling system. PMID: 17405825
    22. The result found that pyramidal neurons from CA1 to CA4 region and granule neurons in dentate gyrus could express FSH and its receptor, majority of hippocampal neurons co-expressed FSH and its receptor, FSH and GnRH receptor. PMID: 17674129
    23. Smad3 and Pitx2 cooperate in stimulation of FSHbeta gene transcription. PMID: 18022758
    24. PI3-kinase may be involved in the negative regulation of alpha- and FSHbeta-subunit gene expression as well as cell proliferation. PMID: 18206295
    25. Dexamethasone (4 nM) for 3 weeks partially rescued LH beta/follicle-stimulating hormone beta cells. PMID: 18363807

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  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Glycoprotein hormones subunit beta family
  • 數據庫鏈接：


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