guinea pig matrix metalloproteinase 9,MMP-9 ELISA Kit
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中文名稱:豚鼠基質金屬蛋白酶9(MMP-9)酶聯免疫試劑盒
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貨號:CSB-E06765Gu
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規格:96T/48T
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價格:¥4200/¥3000
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其他:
產品詳情
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產品描述:豚鼠基質金屬蛋白酶9(MMP-9)酶聯免疫試劑盒(CSB-E06765Gu)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的MMP-9含量。MMP - 9是一種基質金屬蛋白酶,在細胞外基質降解和重塑中發揮關鍵作用。其表達異常與腫瘤生長、轉移、血管生成以及多種炎癥疾病相關。研究機制主要聚焦于其激活調控、底物識別,以及與其他信號通路的相互作用,以探索治療相關疾病的新策略。試劑盒檢測范圍為3.12 ng/mL-200 ng/ mL,適用于科研領域中對炎癥模型評估、組織再生機制研究或心血管疾病相關實驗的定量分析;為豚鼠模型在病理生理機制探索及藥物干預效果評價提供可靠工具本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
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縮寫:MMP-9
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Uniprot No.:
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種屬:Guinea Sus scrofa (Pig)
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樣本類型:serum, plasma, tissue homogenates
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檢測范圍:3.12 ng/mL-200 ng/ mL
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靈敏度:0.78 ng/mL
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反應時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領域:Cancer
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of guinea pig MMP-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 93 Range % 88-98 1:2 Average % 101 Range % 91-104 1:4 Average % 90 Range % 85-93 1:8 Average % 98 Range % 91-102 -
回收率:
The recovery of guinea pig MMP-9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 94 87-97 EDTA plasma (n=4) 87 81-92 -
標準曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. ng/ml OD1 OD2 Average Corrected 200 2.252 2.188 2.220 2.071 100 1.635 1.676 1.656 1.507 50 0.947 0.931 0.939 0.790 25 0.619 0.639 0.629 0.480 12.5 0.341 0.338 0.340 0.191 6.25 0.236 0.231 0.234 0.085 3.12 0.185 0.179 0.182 0.033 0 0.150 0.148 0.149 -
數據處理:
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貨期:3-5 working days
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