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Sheep Transforming growth factor beta-1(TGFB1) ELISA kit

  • 中文名稱:
    綿羊轉化生長因子β1(TGFB1)酶聯免疫試劑盒
  • 貨號:
    CSB-EL023446SH
  • 規格:
    96T/48T
  • 價格:
    ¥4200/¥3000
  • 其他:

產品詳情

  • 產品描述:
    綿羊轉化生長因子β1(TGFB1)酶聯免疫試劑盒(CSB-EL023446SH)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿樣本中的TGFB1含量。TGFB1是重要靶點。它是轉化生長因子,在細胞生長、分化等多種生理活動中起關鍵作用。研究機制方面,其信號通路異常與腫瘤、纖維化等疾病密切相關,當前致力于通過藥物干預該通路,以實現疾病的治療和控制。試劑盒檢測范圍為15.6 ng/mL-1000 ng/mL,適用于綿羊免疫調控機制、炎癥模型構建、生殖生理或疾病相關分子機制等科研領域,可用于探索TGF-β1在動物模型中的表達調控及生物學功能。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    TGFB1 ELISA Kit; Transforming growth factor beta-1 proprotein [Cleaved into: Latency-associated peptide ELISA Kit; LAP); Transforming growth factor beta-1 ELISA Kit; TGF-beta-1)] ELISA Kit
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Ovis aries (Sheep)
  • 樣本類型:
    serum, plasma
  • 檢測范圍:
    15.6 ng/mL-1000 ng/mL
  • 靈敏度:
    3.9 ng/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Signal Transduction
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of sheep TGFB1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)
    1:1 Average % 93
    Range % 86-102
    1:2 Average % 90
    Range % 82-99
    1:4 Average % 95
    Range % 91-99
    1:8 Average % 97
    Range % 93-110
  • 回收率:
    The recovery of sheep TGFB1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 96 89-101
    EDTA plasma (n=4) 97 87-105
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    1000 2.695 2.634 2.665 2.534
    500 2.127 2.098 2.113 1.982
    250 1.429 1.431 1.430 1.299
    125 0.775 0.798 0.787 0.656
    62.5 0.446 0.454 0.450 0.319
    31.25 0.308 0.325 0.317 0.186
    15.6 0.251 0.262 0.257 0.126
    0 0.125 0.136 0.131  
  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

靶點詳情

  • 功能:
    Transforming growth factor beta-1 proprotein: Precursor of the Latency-associated peptide (LAP) and Transforming growth factor beta-1 (TGF-beta-1) chains, which constitute the regulatory and active subunit of TGF-beta-1, respectively.; Required to maintain the Transforming growth factor beta-1 (TGF-beta-1) chain in a latent state during storage in extracellular matrix. Associates non-covalently with TGF-beta-1 and regulates its activation via interaction with 'milieu molecules', such as LTBP1, LRRC32/GARP and LRRC33/NRROS, that control activation of TGF-beta-1. Interaction with LRRC33/NRROS regulates activation of TGF-beta-1 in macrophages and microglia. Interaction with LRRC32/GARP controls activation of TGF-beta-1 on the surface of activated regulatory T-cells (Tregs). Interaction with integrins (ITGAV:ITGB6 or ITGAV:ITGB8) results in distortion of the Latency-associated peptide chain and subsequent release of the active TGF-beta-1.; Multifunctional protein that regulates the growth and differentiation of various cell types and is involved in various processes, such as normal development, immune function, microglia function and responses to neurodegeneration. Activation into mature form follows different steps: following cleavage of the proprotein in the Golgi apparatus, Latency-associated peptide (LAP) and Transforming growth factor beta-1 (TGF-beta-1) chains remain non-covalently linked rendering TGF-beta-1 inactive during storage in extracellular matrix. At the same time, LAP chain interacts with 'milieu molecules', such as LTBP1, LRRC32/GARP and LRRC33/NRROS that control activation of TGF-beta-1 and maintain it in a latent state during storage in extracellular milieus. TGF-beta-1 is released from LAP by integrins (ITGAV:ITGB6 or ITGAV:ITGB8): integrin-binding to LAP stabilizes an alternative conformation of the LAP bowtie tail and results in distortion of the LAP chain and subsequent release of the active TGF-beta-1. Once activated following release of LAP, TGF-beta-1 acts by binding to TGF-beta receptors (TGFBR1 and TGFBR2), which transduce signal. While expressed by many cells types, TGF-beta-1 only has a very localized range of action within cell environment thanks to fine regulation of its activation by Latency-associated peptide chain (LAP) and 'milieu molecules'. Plays an important role in bone remodeling: acts as a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. Can promote either T-helper 17 cells (Th17) or regulatory T-cells (Treg) lineage differentiation in a concentration-dependent manner. At high concentrations, leads to FOXP3-mediated suppression of RORC and down-regulation of IL-17 expression, favoring Treg cell development. At low concentrations in concert with IL-6 and IL-21, leads to expression of the IL-17 and IL-23 receptors, favoring differentiation to Th17 cells. Stimulates sustained production of collagen through the activation of CREB3L1 by regulated intramembrane proteolysis (RIP). Mediates SMAD2/3 activation by inducing its phosphorylation and subsequent translocation to the nucleus. Can induce epithelial-to-mesenchymal transition (EMT) and cell migration in various cell types.
  • 基因功能參考文獻:
    1. In the 2 different muscle tissues, expression of Smad2 was significantly positively correlated (P < 0.01) with that of Smad3. The expression of Smad3 was significantly positively correlated (P < 0.01) with that of Smad4, which showed that the Smad family genes could have an inhibitory effect on the TGF-beta/Smad signaling pathway. PMID: 27323083
    2. Mutations in transforming Growth Factor beta is associated with ovulation PMID: 23801782
    3. TGFbeta signaling can regulate Inhibitor of differentiation gene expression in the sheep ovarian follicle. PMID: 20032053
    4. mRNA encoding both TGF-beta 1 and 2 were synthesized by ovarian theca, stroma and cells of the vascular system whereas TGF-beta 3 mRNA was synthesized by vascular cells PMID: 15563738
    5. antenatal inflammation-induced TGF-beta1 expression and Smad signaling in the fetal lung may contribute to impaired lung alveolarization and reduced lung inflammation PMID: 16936247
    6. Significant differences in mitogen-specific lymphocyte proliferation in vitro and in expression of TGF-beta1 and IL-10 genes in hepatic lymph nodes were observed at acute and chronic phases of infection, respectively. PMID: 19751473

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  • 亞細胞定位:
    [Latency-associated peptide]: Secreted, extracellular space, extracellular matrix.; [Transforming growth factor beta-1]: Secreted.
  • 蛋白家族:
    TGF-beta family
  • 數據庫鏈接:

    KEGG: oas:443417

    UniGene: Oar.393



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