Sheep Serum Amyloid A,SAA ELISA Kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

綿羊血清淀粉樣蛋白A(SAA)酶聯(lián)免疫試劑盒（CSB-E14973Sh）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的SAA1含量。試劑盒檢測范圍為1.875 ng/mL-120 ng/mL，特別適用于畜牧科研領(lǐng)域，可用于綿羊炎癥性疾病模型建立、疫苗免疫效果評(píng)價(jià)、抗炎藥物篩選等基礎(chǔ)研究，亦能為動(dòng)物病理學(xué)研究中組織炎癥程度的定量評(píng)估提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

SAA1 ELISA Kit; Serum amyloid A protein ELISA Kit; SAA ELISA Kit
縮寫：

SAA1
Uniprot No.：

P42819
種屬：

Ovis aries (Sheep)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

1.875 ng/mL-120 ng/mL
靈敏度：

0.47 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Cardiovascular
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of sheep SAA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	94
	Range %	90-98
1:200	Average %	98
	Range %	93-102
1:400	Average %	104
	Range %	98-108
1:800	Average %	89
	Range %	84-93

回收率：

The recovery of sheep SAA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	86	82-91
EDTA plasma (n=4)	94	90-98

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Major acute phase reactant. Apolipoprotein of the HDL complex.
相關(guān)疾病：

Reactive, secondary amyloidosis is characterized by the extracellular accumulation in various tissues of the SAA protein. These deposits are highly insoluble and resistant to proteolysis; they disrupt tissue structure and compromise function.
亞細(xì)胞定位：

Secreted.
蛋白家族：

SAA family
組織特異性：

Expressed by the liver; secreted in plasma.