Sheep 5-hydroxytryptamine/serotonin(5HT/ST) ELISA kit

產(chǎn)品詳情

說(shuō)明書(shū)下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

綿羊5羥色胺/血清素/血清胺(5HT/ST)酶聯(lián)免疫試劑盒（CSB-E14929Sh）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿樣本中的5HT/ST含量。5HT即5-羥色胺，是人體內(nèi)重要神經(jīng)遞質(zhì)。5HT/ST靶點(diǎn)相關(guān)研究聚焦其在調(diào)節(jié)情緒、認(rèn)知等方面作用機(jī)制。通過(guò)藥物作用于該靶點(diǎn)，可調(diào)節(jié)5HT水平與功能，用于治療如抑郁癥、焦慮癥等精神疾病，目前仍在不斷深入探索以挖掘更多臨床價(jià)值。試劑盒檢測(cè)范圍為0.625 ng/mL-10 ng/mL，適用于綿羊模型在神經(jīng)生物學(xué)、藥理學(xué)及動(dòng)物行為學(xué)研究中的應(yīng)用，例如評(píng)估應(yīng)激狀態(tài)下神經(jīng)遞質(zhì)動(dòng)態(tài)變化、藥物干預(yù)對(duì)血清素系統(tǒng)的影響或消化道功能相關(guān)機(jī)制探索本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
縮寫(xiě)：

5HT/ST
種屬：

Ovis aries (Sheep)
樣本類型：

serum, plasma
檢測(cè)范圍：

0.625 ng/mL-10 ng/mL
靈敏度：

0.312 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Biochemicals
測(cè)定原理：

quantitative
測(cè)定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of sheep 5HT/ST in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:20	Average %	84
	Range %	80-92
1:40	Average %	97
	Range %	91-105
1:80	Average %	96
	Range %	90-101
1:160	Average %	93
	Range %	86-98

回收率：

The recovery of sheep 5HT/ST spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-98
EDTA plasma (n=4)	97	90-100

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.