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Rat vascular cell adhesion protein 1 (VCAM-1/CD106) ELISA kit

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產品描述：

大鼠血管內皮細胞粘附分子1(VCAM-1/CD106)酶聯免疫試劑盒（CSB-E07275r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的VCAM1含量。VCAM1，即血管細胞粘附分子1，是一種免疫球蛋白超家族成員，主要表達于活化的血管內皮細胞表面。它通過與白細胞表面的特定分子結合，介導白細胞與內皮細胞的粘附，參與炎癥反應、免疫應答、淋巴細胞歸巢等生理過程。研究顯示，VCAM1在衰老相關疾病如阿爾茨海默病中發揮重要作用，其表達水平與大腦功能衰退密切相關。試劑盒檢測范圍為15.6 ng/mL-1000 ng/mL，適用于炎癥機制研究、心血管疾病動物模型評估、藥物干預效果觀察等科研場景，為探索內皮細胞活化、白細胞遷移等生物學過程提供可靠工具本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。

別名：

Vcam1 ELISA Kit; Vcam-1 ELISA Kit; Vascular cell adhesion protein 1 ELISA Kit; V-CAM 1 ELISA Kit; VCAM-1 ELISA Kit; CD antigen CD106 ELISA Kit

縮寫：

VCAM-1/CD106

Uniprot No.：

P29534

種屬：

Rattus norvegicus (Rat)

樣本類型：

serum, plasma, tissue homogenates

檢測范圍：

15.6 ng/mL-1000 ng/mL

靈敏度：

3.9 ng/mL

反應時間：

1-5h

樣本體積：

50-100ul

檢測波長：

450 nm

研究領域：

Signal Transduction

測定原理：

quantitative

測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat VCAM-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	85-94
1:2	Average %	95
	Range %	86-103
1:4	Average %	96
	Range %	82-105
1:8	Average %	98
	Range %	87-105

回收率：

The recovery of rat VCAM-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-104
EDTA plasma (n=4)	89	85-93

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
1000	2.614	2.704	2.659	2.525
500	2.145	2.236	2.191	2.057
250	1.516	1.536	1.526	1.392
125	0.863	0.848	0.856	0.722
62.5	0.571	0.549	0.560	0.426
31.25	0.352	0.347	0.350	0.216
15.6	0.234	0.244	0.239	0.105
0	0.135	0.132	0.134

本試劑盒所含材料：

A micro ELISA plate --The 96-well plate has been pre-coated with an anti-rat VCAM antibody.This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard --Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled VCAM antibody (100 x concentrate) (120 μl/bottle) --Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) --Dilute the high concentration Biotin-antibody to an appropriate working solution.
One vial HRP-avidin Diluent (15 ml/bottle) --Dilute the high concentration HRP-avidin solution to an appropriate solution.
One vial Sample Diluent (50 ml/bottle)--Dilute the sample to an appropriate concentration.
One vial Wash Buffer (25 x concentrate) (20 ml/bottle) --- Wash away unbound or free substances.
One vial TMB Substrate (10 ml/bottle) --Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
One vial Stop Solution (10 ml/bottle) --Stop the color reaction. The solution color immediately turns from blue to yellow.
Four Adhesive Strips (For 96 wells) --Cover the microplate when incubation.
An instruction manual

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本試劑盒不含材料：

A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
50-300ul multi-channel micropipette
Pipette tips
Single-channel micropipette with different ranges
100ml and 500ml graduated cylinders
Deionized or distilled water
Timer
Test tubes for dilution

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數據處理：

ELISA操作視頻及標曲繪制軟件下載

貨期：

3-5 working days

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.