Rat typeⅠ procollagen,PC I ELISA Kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

大鼠Ⅰ型前膠原(PCⅠ)酶聯(lián)免疫試劑盒（CSB-E11249r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液樣本中的PCⅠ含量。試劑盒檢測范圍為62.5 pg/mL-4000pg/mL，可為研究肝纖維化模型、皮膚再生機(jī)制或骨基質(zhì)代謝等科研領(lǐng)域提供可靠工具，適用于體外實驗中對生物樣本中PCⅠ蛋白水平的精確量化分析，為科研人員探索相關(guān)分子機(jī)制提供高效解決方案。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

type 1 procollagen,PC I,_x000D_ type 1 procollagen,PC I
縮寫：

PCⅠ
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, cell culture supernates
檢測范圍：

62.5 pg/mL-4000pg/mL
靈敏度：

15.6 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat PCⅠ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	93
	Range %	90-96
1:10	Average %	87
	Range %	84-90
1:20	Average %	84
	Range %	81-87
1:40	Average %	92
	Range %	88-96

回收率：

The recovery of rat PCⅠ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	100	97-103
EDTA plasma (n=4)	85	82-89

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.