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中文名稱:大鼠緊密連接蛋白1(ZO1)酶聯免疫試劑盒
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貨號:CSB-E17287r
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規格:96T/48T
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價格:¥3600/¥2500
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其他:
產品詳情
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產品描述:大鼠緊密連接蛋白1(ZO1)酶聯免疫試劑盒(CSB-E17287r)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的ZO1含量。大鼠緊密連接蛋白1(ZO-1)是緊密連接復合體的重要組成部分,參與維持上皮細胞間連接的穩定性和腸道屏障功能。它通過連接膜蛋白與細胞骨架,調節細胞間通透性,對防止病原體和毒素進入體內至關重要。研究顯示,ZO-1的表達與消化系統疾病的發生、發展及預后密切相關,其功能異??赡軐е履c黏膜屏障破壞和多種消化系統疾病。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL,適用于體外研究ZO1在細胞模型或動物模型中的表達調控,例如藥物干預、氧化應激或病原體感染等實驗條件下緊密連接功能的動態評估,為屏障相關疾病的分子機制研究提供可靠工具本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
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別名:DKFZp686M05161, MGC133289, ZO-1, tight junction protein 1|tight junction protein ZO-1|zona occludens 1|zonula occludens 1 protein
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縮寫:ZO1
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種屬:Rattus norvegicus (Rat)
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樣本類型:serum, plasma, tissue homogenates, cell lysates
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檢測范圍:31.25 pg/mL-2000 pg/mL
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靈敏度:7.8 pg/mL
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反應時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領域:Tags & Cell Markers
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of rat ZO1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 91 Range % 86-95 1:2 Average % 102 Range % 97-107 1:4 Average % 91 Range % 85-97 1:8 Average % 97 Range % 91-103 -
回收率:
The recovery of rat ZO1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 95 89-98 EDTA plasma (n=4) 97 90-100 -
標準曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average Corrected 2000 2.272 2.374 2.323 2.221 1000 1.846 1.967 1.907 1.805 500 1.496 1.392 1.444 1.342 250 0.962 0.946 0.954 0.852 125 0.578 0.596 0.587 0.485 62.5 0.364 0.351 0.358 0.256 31.2 0.225 0.230 0.228 0.126 0 0.101 0.102 0.102 -
數據處理:
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貨期:3-5 working days
引用文獻
- Microbiota-derived tryptophan metabolism and AMPK/mTOR pathway mediate antidepressant-like effect of Shugan Hewei Decoction Y Yue, Y Ke, J Zheng, Z Wang, H Liu,Frontiers in Pharmacology,2024
- Activation of AMPK/SIRT1/FOXO3a signaling by BMS-477118 (saxagliptin) mitigates chronic colitis in rats: uncovering new anti-inflammatory and antifibrotic roles EA Elmorsy, ME Youssef, MR Abdel-Hamed,Frontiers in Pharmacology,2024
- Rice bran extract mitigates depressive-like behavior in dextran sulfate sodium-induced colitis: Involvement of the gut-brain axis and Sirt1 signaling pathway HM Shendy,Food and chemical toxicology,2023
- Niacin modulates depressive-like behavior in experimental colitis through GPR109A-dependent mechanisms W Wadie,Life sciences,2023
- (R, R)-BD-AcAc2 Mitigates Chronic Colitis in Rats: A Promising Multi-Pronged Approach Modulating Inflammasome Activity, Autophagy, and Pyroptosis S Saber,Pharmaceuticals,2023
- Gut microbiota-derived melatonin from Puerariae Lobatae Radix-resistant starch supplementation attenuates ischemic stroke injury via a positive microbial co-occurrence pattern Z Lian,Pharmacological research,2023
- Potentilla chinensis aqueous extract attenuates cyclophosphamide-induced hemorrhagic cystitis in rat model K Juszczak,Scientific Reports,2022
- The influence of Potentilla chinensis aqueous extract on urinary bladder function in retinyl acetate-induced detrusor overactivity in rats A Wróbel,Biomedicine & Pharmacotherapy,2020
- Puerariae Lobatae Radix with chuanxiong Rhizoma for treatment of cerebral ischemic stroke by remodeling gut microbiota to regulate the brain-gut barriers RunzhiChen, et al,Journal of nutritional biochemistry,2018
- The combination of Puerariae Lobatae Radix and Chuanxiong Rhizoma enhanced the absorption and pharmacokinetics of puerarin by modulating the intestinal barrier and influenced gut microbiota Runzhi Chen.et al,Journal of Functional Foods,2018
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