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Rat terminal complement complex C5b-9,TCC C5b-9 ELISA Kit

  • 中文名稱:
    大鼠末端補體復合物C5b-9(TCC C5b-9)酶聯免疫試劑盒
  • 貨號:
    CSB-E08709r
  • 規格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 其他:

產品詳情

  • 產品描述:
    大鼠末端補體復合物C5b-9(TCC C5b-9)酶聯免疫試劑盒(CSB-E08709r)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的TCC C5b9含量。大鼠末端補體復合物C5b-9(TCC C5b-9)是補體系統激活后形成的膜攻擊復合物,通過介導細胞膜穿孔參與免疫防御及炎癥反應調控,在免疫相關疾病機制研究中具有重要價值。試劑盒檢測范圍為1.56 ng/mL-100 ng/mL,適用于基礎醫學研究中補體系統功能評估,例如在建立腎小球腎炎、心肌梗死或神經退行性疾病動物模型時,可通過檢測血清或組織中的C5b - 9水平動態觀察補體激活程度;亦可用于評價藥物或生物制劑對補體通路的調控作用。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 縮寫:
    TCC C5b9
  • 種屬:
    Rattus norvegicus (Rat)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    1.56 ng/mL-100 ng/mL
  • 靈敏度:
    0.39 ng/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Others
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rat TCC C5b-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:100Average %91
    Range %86-98
    1:200Average %96
    Range %92-102
    1:400Average %85
    Range %81-88
    1:800Average %88
    Range %83-95
  • 回收率:
    The recovery of rat TCC C5b-9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9490-98
    EDTA plasma (n=4)9087-95
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    1002.351 2.502 2.427 2.282
    502.021 2.165 2.093 1.948
    251.423 1.540 1.482 1.337
    12.50.936 1.002 0.969 0.824
    6.250.607 0.581 0.594 0.449
    3.120.372 0.360 0.366 0.221
    1.560.238 0.257 0.248 0.103
    00.141 0.149 0.145  
  • 數據處理:
  • 貨期:
    3-5 working days

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