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  4. Rat regulated on activation in normal T-cell expressed and secreted/C-C motif chemokine 5 (RANTES/CCL5) ELISA kit

Rat regulated on activation in normal T-cell expressed and secreted/C-C motif chemokine 5 (RANTES/CCL5) ELISA kit

  • 中文名稱：
    大鼠正常T細胞表達和分泌因子/趨化因子5(RANTES/CCL5)酶聯免疫試劑盒
  • 貨號：
    CSB-E07398r-IS
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    大鼠正常T細胞表達和分泌因子/趨化因子5(RANTES/CCL5)酶聯免疫試劑盒（CSB-E07398r-IS）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的RANTES/CCL5含量。RANTES/CCL5屬于趨化因子，在免疫反應等生理病理過程中起重要作用。研究發現它可趨化多種免疫細胞，參與炎癥反應、腫瘤轉移等進程。其作用機制主要是與相應受體結合，激活下游信號通路，引發系列細胞反應。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，靈敏度為7.8 pg/mL。適用于多種基礎科研場景：包括但不限于炎癥性疾病機制研究（如關節炎模型）、免疫應答動態分析、病毒感染相關趨化因子調控實驗（如HIV相關研究），以及藥物開發過程中抗炎或免疫調節化合物的體外效果評估。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    Ccl5 ELISA Kit; Scya5C-C motif chemokine 5 ELISA Kit; SIS-delta ELISA Kit; Small-inducible cytokine A5 ELISA Kit; T-cell-specific protein RANTES ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍：
    15.6 pg/mL-1000 pg/mL
  • 靈敏度：
    7.8 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of rat RANTES/CCL5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:10

    Average %

    99

    Range %

    92-105

    1:20

    Average %

    93

    Range %

    89-98

    1:40

    Average %

    101

    Range %

    96-106

    1:80

    Average %

    90

    Range %

    85-96

  • 回收率：

    The recovery of rat RANTES/CCL5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    96

    89-102

    EDTA plasma (n=4)

    91

    84-98

  • 標準曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    1000

    2.863

    2.914

    2.888

    2.738

    500

    1.685

    1.617

    1.651

    1.501

    250

    0.772

    0.738

    0.755

    0.605

    125

    0.445

    0.429

    0.437

    0.287

    62.5

    0.313

    0.315

    0.314

    0.164

    31.2

    0.225

    0.233

    0.229

    0.079

    15.6

    0.196

    0.189

    0.192

    0.042

    0

    0.152

    0.148

    0.150

    　

  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. May activate several chemokine receptors including CCR1, CCR3, CCR4 and CCR5. May also be an agonist of the G protein-coupled receptor GPR75. Together with GPR75, may play a role in neuron survival through activation of a downstream signaling pathway involving the PI3, Akt and MAP kinases. By activating GPR75 may also play a role in insulin secretion by islet cells.
  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Intercrine beta (chemokine CC) family
  • 數據庫鏈接：


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