Rat platelet membrane glycoprotein ⅡbⅢa,GP-ⅡbⅢa ELISA Kit
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中文名稱:大鼠血小板膜糖蛋白ⅡbⅢa(GP-ⅡbⅢa/CD41+CD61)酶聯免疫試劑盒
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貨號:CSB-E08776r
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規格:96T/48T
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價格:¥3900/¥2500
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其他:
產品詳情
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產品描述:大鼠血小板膜糖蛋白ⅡbⅢa(GP-ⅡbⅢa/CD41+CD61)酶聯免疫試劑盒(CSB-E08776r)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的GPⅡbⅢa/CD41+CD61含量。GPⅡbⅢa/CD41+CD61作為靶點,在血小板功能中至關重要。參與血小板聚集過程,研究機制聚焦于通過阻斷該靶點,抑制血小板的聚集與黏附,進而為抗血栓藥物研發提供方向,以防治相關心血管疾病。試劑盒檢測范圍為6.25 ng/mL-400 ng/mL,適用于多種生物樣本中GP - ⅡbⅢa的精確檢測,可廣泛用于大鼠模型中血小板功能調控機制研究、血栓相關疾病動物模型的病理分析、藥物干預效果評價等科研領域,為探索血小板相關分子機制提供可靠工具。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
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別名:platelet membrane glycoprotein ⅡbⅢa,GP-ⅡbⅢa |platelet membrane glycoprotein 2b3a,GP-2b3a
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縮寫:GPⅡbⅢa/CD41+CD61
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種屬:Rattus norvegicus (Rat)
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樣本類型:serum, plasma, tissue homogenates
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檢測范圍:6.25 ng/mL-400 ng/mL
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靈敏度:1.56 ng/mL
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反應時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領域:Others
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of rat GP-ⅡbⅢa in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 87 Range % 82-92 1:2 Average % 89 Range % 82-95 1:4 Average % 95 Range % 90-100 1:8 Average % 90 Range % 85-98 -
回收率:
The recovery of rat GP-ⅡbⅢa spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 92 87-97 EDTA plasma (n=4) 98 92-105 -
標準曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. ng/ml OD1 OD2 Average Corrected 400 2.176 2.053 2.115 1.921 200 1.855 1.764 1.810 1.616 100 1.542 1.469 1.506 1.312 50 1.236 1.178 1.207 1.013 25 0.837 0.786 0.812 0.618 12.5 0.421 0.393 0.407 0.213 6.25 0.213 0.196 0.205 0.011 0 0.198 0.190 0.194 -
數據處理:
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貨期:3-5 working days
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