Rat neuropeptide Y (NPY) ELISA kit

產品詳情

文獻引用 (3) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

大鼠神經肽Y(NPY)酶聯免疫試劑盒（CSB-E13431r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的NPY含量。NPY即神經肽Y，是一種廣泛分布于中樞和外周神經系統(tǒng)的神經肽。它參與多種生理功能調節(jié)，如攝食、能量代謝、心血管活動等。研究機制方面，主要通過與不同亞型受體結合，激活下游信號通路來發(fā)揮作用，在相關疾病治療研究中是潛在靶點。試劑盒檢測范圍為1.56 pg/mL-100 pg/mL，適用于神經生物學研究、代謝調節(jié)機制探索、藥物干預效果評價等科研領域。支持大鼠模型中的NPY動態(tài)監(jiān)測，例如神經退行性疾病模型、肥胖癥研究或應激反應實驗中的生物標志物分析。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

NPY
Uniprot No.：

P07808
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

1.56 pg/mL-100 pg/mL
靈敏度：

0.39 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Neuroscience
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat NPY in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	92-104
1:2	Average %	97
	Range %	92-103
1:4	Average %	93
	Range %	89-95
1:8	Average %	86
	Range %	82-92

回收率：

The recovery of rat NPY spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	87-99
EDTA plasma (n=4)	105	100-110

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

NPY is implicated in the control of feeding and in secretion of gonadotrophin-release hormone.
亞細胞定位：

Secreted. Cytoplasmic vesicle, secretory vesicle, neuronal dense core vesicle.
蛋白家族：

NPY family
組織特異性：

One of the most abundant peptides in the nervous system. Also found in some chromaffin cells of the adrenal medulla.
數據庫鏈接：

KEGG: rno:100912228

STRING: 10116.ENSRNOP00000013146

UniGene: Rn.9714