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Rat myeloperoxidase-antineutrophil cytoplasmic antibody(MPO-ANCA) ELISA kit

  • 中文名稱:
    大鼠髓過氧化物酶特異性抗中性粒細胞胞質抗體(MPO-ANCA)酶聯免疫試劑盒
  • 貨號:
    CSB-E08675r
  • 規格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 其他:

產品詳情

  • 產品描述:
    大鼠髓過氧化物酶特異性抗中性粒細胞胞質抗體(MPO-ANCA)酶聯免疫試劑盒(CSB-E08675r)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿樣本中的MPO-ANCA含量。MPO-ANCA(髓過氧化物酶特異性抗中性粒細胞胞漿抗體)是一種與血管炎和腎小球腎炎密切相關的自身抗體。其研究機制主要涉及MPO分子被自身免疫性T/B細胞識別的抗原表位,以及易感HLA分子、補體活化、凝血和血小板等參與的免疫炎癥反應。MPO-ANCA的致病性在動物模型和臨床研究中得到證實,其與疾病活動度密切相關,是治療的關鍵靶點。試劑盒檢測范圍為0.625 U/mL-40 U/mL,該產品適用于科研場景中血管炎動物模型的抗體水平動態監測、疾病機制探索以及藥物干預效果評價等研究,為深入解析MPO - ANCA在免疫病理過程中的作用提供可靠工具。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    N/A
  • 縮寫:
    MPO-ANCA
  • 種屬:
    Rattus norvegicus (Rat)
  • 樣本類型:
    serum, plasma
  • 檢測范圍:
    0.625 U/mL-40 U/mL
  • 靈敏度:
    0.156 U/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Immunology
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rat MPO-ANCA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1Average %85
    Range %80-95
    1:2Average %97
    Range %91-105
    1:4Average %96
    Range %92-100
    1:8Average %93
    Range %85-98
  • 回收率:
    The recovery of rat MPO-ANCA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9489-97
    EDTA plasma (n=4)9184-96
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    U/mlOD1OD2AverageCorrected
    402.612 2.752 2.682 2.518
    202.013 2.076 2.045 1.881
    101.367 1.304 1.336 1.172
    50.802 0.837 0.820 0.656
    2.50.444 0.424 0.434 0.270
    1.250.351 0.359 0.355 0.191
    0.6250.241 0.225 0.233 0.069
    00.160 0.167 0.164  
  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

相關問答

 常見問題解答
Q:

Can you please confirm the following
1) Standard - Is it a recombinant or not? If it's recombinant, please let us know the origin. ( for ex;E-coli, bculovirus).
2) Antibodies → Please let us know whether the antibodies include NaN3, Thimerosal or 2-Me as preservative. If it have any preservatives, please give us the content(%)
3) Stop Solution → Please let us know the constituent and concentration. We would like to confirm about the toxicity.

A:
Thanks for your inquiry.
CSB-E08675r
This kit is for quantitative determination of antibody.
Standard is crude extract from natural samples.
Stop solution is 4N sulfuric acid.
Preservative is proclin 300(1:3000).
The kit doesn't contain NaN3, Thimerosal or 2-Me.
Pls let me know if you have any further questions. Thank you.


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