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  4. Rat monoamine oxidase(MAO) ELISA Kit

Rat monoamine oxidase(MAO) ELISA Kit

  • 中文名稱：
    大鼠單胺氧化酶(MAO)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E15076r
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠單胺氧化酶(MAO)酶聯(lián)免疫試劑盒（CSB-E15076r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的MAO含量。MAO（單胺氧化酶）是一種催化單胺類物質(zhì)氧化脫氨反應(yīng)的酶，存在于細(xì)胞線粒體外膜上，廣泛分布于人體肝、腦、腎等組織細(xì)胞內(nèi)。其研究機(jī)制涉及酶的結(jié)構(gòu)、活性位點(diǎn)、催化過程等，對理解生物體內(nèi)胺濃度調(diào)節(jié)及相關(guān)疾病有重要意義。試劑盒檢測范圍為93.75 mU/mL-6000 mU/mL，適用于神經(jīng)科學(xué)、藥理學(xué)等領(lǐng)域的基礎(chǔ)研究，例如探究藥物干預(yù)或疾病模型下MAO活性變化、評估神經(jīng)遞質(zhì)代謝調(diào)控機(jī)制等科研場景，為探索神經(jīng)系統(tǒng)相關(guān)疾病的分子機(jī)制提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Maoa; Amine oxidase [flavin-containing] A; Monoamine oxidase type A; MAO-A
  • 縮寫：
    MAO
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍：
    93.75 mU/mL-6000 mU/mL
  • 靈敏度：
    23.43 mU/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Neuroscience
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat MAO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:5Average %92
    Range %88-96
    1:10Average %96
    Range %90-102
    1:20Average %102
    Range %98-104
    1:40Average %87
    Range %83-92
  • 回收率：
    The recovery of rat MAO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9285-99
    EDTA plasma (n=4)8982-96
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mU/mlOD1OD2AverageCorrected
    60002.633 2.866 2.750 2.557
    30001.787 1.881 1.834 1.641
    15001.123 1.188 1.156 0.963
    7500.689 0.705 0.697 0.504
    3750.502 0.525 0.514 0.321
    187.50.369 0.360 0.365 0.172
    93.750.245 0.236 0.241 0.048
    00.195 0.191 0.193
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

相關(guān)問答

■ 常見問題解答
Q:

May I know the composition of stop solution that is used for the monoamine oxidase ELISA Kit?

A:
Thanks for your inquiry. The stop solution of this kit is 4N sulfuric acid.Pls let me know if you have any further questions. Thank you.

靶點(diǎn)詳情

  • 功能：
    Catalyzes the oxidative deamination of biogenic and xenobiotic amines and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. MAOA preferentially oxidizes biogenic amines such as 5-hydroxytryptamine (5-HT), norepinephrine and epinephrine.
  • 亞細(xì)胞定位：
    Mitochondrion outer membrane; Single-pass type IV membrane protein; Cytoplasmic side.
  • 蛋白家族：
    Flavin monoamine oxidase family
  • 數(shù)據(jù)庫鏈接：


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